LOCUS       sequence-471512-        2831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Homology psbH reversee in the M2 position used for genome 
            integration at the psbH locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_6_006_psbH_5'Hom_rev_Cr
FEATURES             Location/Qualifiers
     source          1..2831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(376..470)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(491..1150)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1065..1084
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1312..1330
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1334..1377)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1380..1401
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2184..2241
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2265..2282)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2436..2831,1..193))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2516..2535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
       61 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      121 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
      181 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
      241 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
      301 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
      361 cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
      421 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
      481 cgatatcaaa ttacgccccg ccctgccact cgtcgcagta ctgttgtaat tcattaagca
      541 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
      601 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
      661 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgatacga
      721 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
      781 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagtg
      841 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
      901 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
      961 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     1021 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     1081 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     1141 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     1201 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga
     1261 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     1321 atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg
     1381 aattcgcggc cgcttctaga gcgatgagtg gtctcgcgct cgttaagata aacgcgttaa
     1441 tagctcactt ttctttaaat ttaattttta atttaaaggt gtaagcaaat tgcctgacga
     1501 gagatccact taaaggatga cagtggcggg ctactgccta cttccctccg ggataaaatt
     1561 tatttgaaaa acgttagtta cttcctaacg gagcattgac atccccatat ttatattagg
     1621 acgtcccctt cgggtaaata aattttagtg gacgtcccct tcgggcaaat aaattttagt
     1681 ggacaataaa taaatttgtt gcctgccaac tgcctaggca agtaaacttg ggagtattaa
     1741 aataggacgt cagtggcagt tgcctgccaa ctgcctatat ttatatactg cgaagcaggc
     1801 agtggcggta ccactgccac tggcgtccta atataaatat tgggcaacta aagtttatag
     1861 cagtattaac atcctatatt tatatactcc gaaggaactt gttagccgat aggcgaggca
     1921 acaaatttat ttattgtccc gtaaaaggat gcctccagca tcgaagggga aggggacgtc
     1981 ctaggccata aaactaaagg gaaatccata gtaactgatg ttataaattt atagactcca
     2041 aaaaacagct gcgttataaa taacttctgt taaatatggc caaggggaca ggggcacttt
     2101 caactaagtg tacattaaaa attgacaatt ctgttggaga cgagactgag accactcatc
     2161 gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct
     2221 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
     2281 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2341 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2401 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
     2461 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2521 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2581 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2641 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2701 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2761 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2821 gtaggcggtg c
//
