LOCUS       sequence-471514-        3908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' Homology petB in the M2 position used for genome integration at 
            the petB locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_6_013_petB_3'Hom_Cr
FEATURES             Location/Qualifiers
     source          1..3908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1569..1626
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1650..1667)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1821..2409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1901..1920)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2592..2686)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2707..3366)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3281..3300
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3528..3546
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3550..3593)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3596..3617
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gcaagtaaaa gaaatggata ttaaatatta tagaggacct gaaatacctt gtttacgaat
       61 cattaagaag tgcattaaca tgaaaacagc tgttaaaagt ggtaatacga aagtgtgtaa
      121 actgtagaaa cgtgttaaag ttgcttgacc aacaccaaca ccaccacgta ataactcaac
      181 aatgaaacca ccaacacctg ggattgcatc aggaacacct gttacaattt taaccgccca
      241 gtaaccaact tggtcccatg gtaatgaata acctgttaca ccaaaagaaa ctgtacatac
      301 agccatgatt acacctgtaa cccatgttaa ttcacgtgga cgtttgaaac cacctgttaa
      361 atatacacgg aaaacgtgta aaaccatcat aagaaccatc atactagctg accaacggtg
      421 aattgaacga attaaccaac caaagttaac atcagtcata atgtattgta ctgatgcgaa
      481 agcttctgct actgttggac ggtagtagaa agtcatagca aaaccagtag ctacttgcac
      541 aaggaaacat gtaaaagtaa taccaccaat acagtagaaa atatttacgt gtggtggaac
      601 atatttactt gtaatatcat cagcaattgc ttgaatttct aaacgttctt caaaccaatc
      661 gtatacttta ctcatataaa atttttataa gattgtgaca tgaccattag gctttcttaa
      721 gactaaaaaa atgtgtagct taattattta aagtttaaat taaagttata atatatatta
      781 tatataaaat aaaaaaaacg ttagtaattc aaaagtttta atattataca attgaactat
      841 tatgtattaa atataagaat gtcacctctt accatatttc tatactccaa agtaactttt
      901 tacataaatg tcccctctgg ggctgcctcc ttccccttcc ccttcgggta tataaatata
      961 gggcaagtaa acttagcata aactttagtt gcccgaaggg gtttacatac tccgaaggag
     1021 gacaaattta tttattgtgg tacaataaat aaattgtatg taaacccctt tcgggtaact
     1081 aaagtttatc acggcaataa gtttctgctt acgcagtatt atatctgacg cagtattata
     1141 taagaagttg gcaggataaa aatgtgtaag tatggcaatc ttttaaaata gtgttcaatt
     1201 catttaaggc agataaaaag aaaaaagtcc acaggattta attttgaata gttctctatc
     1261 aaaaaaaggt ttgccgaaca atgtttttat tcctggagtt tgattttatg aaattagctg
     1321 tttacggaaa aggtggtatt ggaaaatcaa cgacaagttg taatatttcg attgctttag
     1381 caaaacgtgg taaaaaagtg ttacaaattg gttgtgatcc taaacatgat agtactttta
     1441 cattgacagg gtttttaatt ccaaccatta ttgatacatt aagttctaaa gattatcatt
     1501 atgaagatat ttggccagac tgagacgaga ctgagaccac tcatcgcgac tagtagcggc
     1561 cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
     1621 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1681 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1741 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1801 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
     1861 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1921 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1981 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2041 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2101 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2161 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2221 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2281 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2341 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2401 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2461 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2521 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     2581 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     2641 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     2701 atcaaattac gccccgccct gccactcgtc gcagtactgt tgtaattcat taagcattct
     2761 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     2821 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     2881 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg atacgaaaaa
     2941 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     3001 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagtgatga
     3061 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     3121 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     3181 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     3241 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     3301 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     3361 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     3421 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
     3481 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     3541 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
     3601 cgcggccgct tctagagcga tgagtggtct cgcgctcttg tcgaaataaa tagcacactc
     3661 ttgcatatct atattttaag atgtcagctt tttgattcat tgagttgtat attttattta
     3721 ctttactagg gtgtttatat attccttttt ggggttgccc agatagttat ataaccaatc
     3781 acaacaacag taaaaattga agtttattta cccaaagggg tgtatcccct ttgggtaaat
     3841 aaacttcaat ggccaactgc cttggaaact tacgtagcag cctaaaaaaa gctagtctta
     3901 tatccgaa
//
