LOCUS       sequence-471515-        3384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' Homology psbD in the M2 position used for genome integration at 
            the psbD locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_6_019_psbD_3'Hom_Cr
FEATURES             Location/Qualifiers
     source          1..3384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      140..234
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      417..1005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     906..925
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1159..1176
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1200..1257)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    2593..2614
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2617..2660
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2664..2682)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(2844..3384,1..119)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2910..2929)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
       61 tcggcagaat gcttaatgaa ttacaacagt actgcgacga gtggcagggc ggggcgtaat
      121 ttgatatcga gctcgcttgg actcctgttg atagatccag taatgacctc agaactccat
      181 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc
      241 ctcgagctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
      301 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      361 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      421 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      481 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      541 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      601 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      661 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      721 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      781 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      841 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      901 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      961 cgtcgatttt tgtgatgctc gtcagggggg cggagcctgt ggaaaaacgc cagcaacgcg
     1021 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1081 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1141 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cctgcataac gcgaagtaat
     1201 cttttcggtt ttaaagaaaa agggcagggt ggtgacacct tgcccttttt tgccggactg
     1261 cagcggccgc tactagtcgc gatgagtggt ctcagtctcg tctcagtctc taaatctcca
     1321 atttgcaatt tagagatata attaaaacta taaagttatt taagttaatt tgtaatcaaa
     1381 tccaacacaa aaatgttttt atatagttaa catgttaaat ttaacatatg ttaaacaact
     1441 aaaattctgt aacagagaac aataaaataa atgctagatt ttgtgtaatg ccgaagtata
     1501 tttatatact tccctttcaa aaaaataaat actcttgcca ctaaaattca tttgcctagg
     1561 acgtcccctt ccccttacgg gatgtttata tactaggacg tccccttccc cttacgggat
     1621 atttatatac tccgaaggac gtccccttcg ggcaaataaa ttttagtggc agttgcctgc
     1681 caactgccta ggcaagtaaa cttagggatt ttaatgcaat aaataaattt gtccccttac
     1741 gggacgtcag tggcagttgc ctgccaactg cctaatataa atattagtgg atatttatat
     1801 actccgaagg aggcagttac ctgccaactg ccgaggcaaa taaattttag tggcagtggt
     1861 accgccactg cctgctccct ccttcccctt cgggcaagta aacttagcat gtttacatac
     1921 tccgaaggag gacaaattta tttattgtgg tacaataaat aagtggtaca ataaataaat
     1981 tgtatgtaaa ccccttcccc ttcgggacgt ccccttacgg gaatataaat attagtggca
     2041 gttgcctgcc aacaaattta tttattgtat taacataggc agtggcggta ccactgccac
     2101 tggcgtccta atataaatat tgggcaacta aagtttatcg cagtattaac ataggcagtg
     2161 gcggtaccac tgccactggc gtcctccttc ggagtatgta aacctgctac cgcagcaaat
     2221 aaattttatt ctattttaat actacaatat ttagattccc gttaggggat aggccaggca
     2281 attgtcactg gcgtcatagt atatcaatat tgtaacagat tgacaccctt taagtaaaca
     2341 ttttttttag gattcatatg aaattaaatg gatatttggt acatttaatt ccacaaaaat
     2401 gtccaatact taaaatacaa aattaaaagt attagttgta aacttgacta acattttaaa
     2461 ttttaaattt tttcctaatt atatatttta cttgcaaaat ttataaaaat tttatgcatt
     2521 tttatatcat aataataaaa cctttattca tggtttataa tataataatt gtgagcgcga
     2581 gaccactcat cgctctagaa gcggccgcga attccagaaa tcatccttag cgaaagctaa
     2641 ggattttttt tatctgaaat tctgcctcgt gatacgccta tttttatagg ttaatgtcat
     2701 gataataatg gtttcttaga cgtcaggtgg cactcgagtt gatcgggcac gtaagaggtt
     2761 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
     2821 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     2881 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     2941 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     3001 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     3061 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     3121 ccgttttcca tgagcaaact gaaacgtttt catcactctg gagtgaatac cacgacgatt
     3181 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     3241 atttccctaa agggtttatt gagaatatgt ttttcgtatc agccaatccc tgggtgagtt
     3301 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     3361 tgggcaaata ttatacgcaa ggcg
//
