LOCUS       sequence-471516-        2922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5'Homology petB reverse in the M2 position used for genome 
            integration at the petB locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_6_021_petB_5'Hom_rev_Cr
FEATURES             Location/Qualifiers
     source          1..2922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    800..821
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      824..867
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(871..889)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1051..1710
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1117..1136)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1731..1825
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2008..2596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2497..2516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2750..2767
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2791..2848)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 cgccagataa attttctttt tttactgtat cttttccttt tgtttttgcg gtgccacttt
       61 taaataatcc acttttttcc atttcttttt tttccaactc tttagaacga tcttccatat
      121 ggatattaat taataaacca caaatttggt tacataattc atcatctaaa tattgcatta
      181 aaaataccat acgacgacgg aaaataaagt tataaatatc agtccactgt gcaggtaatt
      241 cttcacccca acaataaata atacgaggta ctccaatcgg cataaattac tttgttaata
      301 aaatgttgtg ttttattaat actagtctaa tatcctagta gatagagctt ttatttgcac
      361 gtaaataagc tctgcgagtg ctactgtaaa aattaaagta ccgtttacag gctcctttga
      421 agcaaataaa aattttaaac caaaatggtt taaaaaatag ataaattcaa acaaatattg
      481 gctctacgtt aaacttaaaa tgccttcggc cggatttgaa ccggcacgcc tttcagcact
      541 ggttcctaaa accaggatgt ctaccagttc catcacgaag gctaaaaaat attactttta
      601 taatatcata cttaattttt tttgtctagt taaaaacact aaatgtgtta aatgcataca
      661 caattttttg tttagactag agacttgggg ttagttccga tcctttaacc agaatataac
      721 tatggttaat ttatagctac cccttatcaa tgggccaacg ggggatctgg cagaaaccgc
      781 agcgcgagac cactcatcgc tctagaagcg gccgcgaatt ccagaaatca tccttagcga
      841 aagctaagga ttttttttat ctgaaattct gcctcgtgat acgcctattt ttataggtta
      901 atgtcatgat aataatggtt tcttagacgt caggtggcac tcgagttgat cgggcacgta
      961 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
     1021 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
     1081 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
     1141 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
     1201 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
     1261 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
     1321 tgttacaccg ttttccatga gcaaactgaa acgttttcat cactctggag tgaataccac
     1381 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
     1441 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtatcagc caatccctgg
     1501 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
     1561 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
     1621 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
     1681 tactgcgacg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt
     1741 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
     1801 ccgggcgttt tttattggtg agaatccaag cctcgagctg tcagaccaag tttactcata
     1861 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1921 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1981 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2041 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2101 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2161 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2221 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2281 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2341 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2401 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2461 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2521 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2581 gcggagcctg tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2641 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2701 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2761 gagcgaggaa gcctgcataa cgcgaagtaa tcttttcggt tttaaagaaa aagggcaggg
     2821 tggtgacacc ttgccctttt ttgccggact gcagcggccg ctactagtcg cgatgagtgg
     2881 tctcagtctc gtctcgaaca gattgacgtt ttgcactagc gc
//
