LOCUS       sequence-471523-        2529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' Connector 1 Long Reverse in the M2 position used to dictate 
            position for lvl2 assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_0_6_004_3C1LR
FEATURES             Location/Qualifiers
     source          1..2529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(84..103)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(775..869)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(890..1549)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1464..1483
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1711..1729
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1733..1776)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1779..1800
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2281..2338
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2362..2379)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
       61 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      121 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      181 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      241 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      301 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      361 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      421 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      481 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      541 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      601 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      661 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      721 taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc
      781 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
      841 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
      901 cctgccactc gtcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
      961 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1021 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1081 caaaactggt gaaactcacc cagggattgg ctgatacgaa aaacatattc tcaataaacc
     1141 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     1201 gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt tcagtttgct
     1261 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     1321 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     1381 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     1441 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     1501 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     1561 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     1621 ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa
     1681 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
     1741 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag
     1801 cgatgagtgg tctcgcgctg gaccaaaacg aaaaaagacg cttttcagcg tcttattgtt
     1861 cgtctttggt accgagctgt gtctgtggct gagagagcag gtacaagata aacccgtcta
     1921 tattctcaaa ttctttcact atcgcacttg ttttatgtga agtccagata tggactctgg
     1981 aacccaactc ggagctctgc gggtagagcg tcaaccattc gaacgttgcg cggtcttcgg
     2041 agaaccgtaa gaggtaatat actccgatat tcatttcagc ggtagctgag tccgtcagtt
     2101 gatacggtgc gtacccggag caaataggag aggcggttgt agtgggggga atggatagca
     2161 agctgcgggt agacctccaa ttattgaagg cctcccaaat cggggggcct tttttattga
     2221 taacaaaatg ttggagacga gactgagacc actcatcgcg actagtagcg gccgctgcag
     2281 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
     2341 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2401 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2461 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2521 gttgctggc
//
