LOCUS       sequence-471526-        2120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' Connector 3 Short Reverse in the M2 position used to dictate 
            position for lvl2 assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_0_6_012_3C3SR
FEATURES             Location/Qualifiers
     source          1..2120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     189..206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(230..287)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    359..380
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      383..426
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(430..448)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             610..1269
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(676..695)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1290..1384
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     2056..2075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtgatgctc gtcagggggg cggagcctgt ggaaaaacgc cagcaacgcg gcctttttac
       61 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      121 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      181 ccgagcgcag cgagtcagtg agcgaggaag cctgcataac gcgaagtaat cttttcggtt
      241 ttaaagaaaa agggcagggt ggtgacacct tgcccttttt tgccggactg cagcggccgc
      301 tactagtcgc gatgagtggt ctcagtctcg tctccaatga gcgcgagacc actcatcgct
      361 ctagaagcgg ccgcgaattc cagaaatcat ccttagcgaa agctaaggat tttttttatc
      421 tgaaattctg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      481 cttagacgtc aggtggcact cgagttgatc gggcacgtaa gaggttccaa ctttcaccat
      541 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
      601 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
      661 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
      721 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
      781 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg
      841 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
      901 caaactgaaa cgttttcatc actctggagt gaataccacg acgatttccg gcagtttcta
      961 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     1021 tttattgaga atatgttttt cgtatcagcc aatccctggg tgagtttcac cagttttgat
     1081 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     1141 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     1201 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgacga gtggcagggc
     1261 ggggcgtaat ttgatatcga gctcgcttgg actcctgttg atagatccag taatgacctc
     1321 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
     1381 gaatccaagc ctcgagctgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1441 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1501 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1561 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1621 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1681 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1741 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1801 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1861 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1921 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1981 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2041 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2101 ctgacttgag cgtcgatttt
//
