LOCUS       sequence-471532-        2122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' Operon Connector 4 in the M2 position used to construct operons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_0_5-6_009_OC3'4
FEATURES             Location/Qualifiers
     source          1..2122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8..27)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(699..793)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(814..1473)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1388..1407
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1635..1653
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1657..1700)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1703..1724
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1798..1855
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1879..1896)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2050..2122,1..516))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
       61 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      361 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
      601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      661 atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc
      721 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
      781 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcgtcgca
      841 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      901 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      961 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1021 cacccaggga ttggctgata cgaaaaacat attctcaata aaccctttag ggaaataggc
     1081 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1141 gtcgtggtat tcactccaga gtgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1201 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1261 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1321 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1381 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1441 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1501 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1561 tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga
     1621 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
     1681 ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagcgatga gtggtctcgg
     1741 cttaacaagg gagacgagac tgagaccact catcgcgact agtagcggcc gctgcagtcc
     1801 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
     1861 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1921 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1981 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2041 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2101 tcagaggtgg cgaaacccga ca
//
