LOCUS       sequence-471533-        2122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' Operon Connector 5 in the M2 position used to construct operons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_0_5-6_010_OC3'5
FEATURES             Location/Qualifiers
     source          1..2122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(269..326)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    400..421
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      424..467
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(471..489)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             651..1310
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(717..736)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1331..1425
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(1608..2122,1..74)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2097..2116
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
       61 ggagcctgtg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      121 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      181 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      241 gcgaggaagc ctgcataacg cgaagtaatc ttttcggttt taaagaaaaa gggcagggtg
      301 gtgacacctt gccctttttt gccggactgc agcggccgct actagtcgcg atgagtggtc
      361 tcagtctcgt ctcctacctt aagccgagac cactcatcgc tctagaagcg gccgcgaatt
      421 ccagaaatca tccttagcga aagctaagga ttttttttat ctgaaattct gcctcgtgat
      481 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      541 tcgagttgat cgggcacgta agaggttcca actttcacca taatgaaata agatcactac
      601 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa
      661 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
      721 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
      781 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
      841 cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga
      901 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
      961 cactctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     1021 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     1081 tcgtatcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     1141 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     1201 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
     1261 tgcttaatga attacaacag tactgcgacg agtggcaggg cggggcgtaa tttgatatcg
     1321 agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
     1381 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag cctcgagctg
     1441 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     1501 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     1561 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1621 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1681 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1741 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1801 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1861 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1921 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1981 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2041 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2101 aacgcctggt atctttatag tc
//
