LOCUS       sequence-471535-        2122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' Operon Connector 7 in the M2 position used to construct operons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_0_5-6_012_OC3'7
FEATURES             Location/Qualifiers
     source          1..2122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2..45
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(49..67)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             229..888
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(295..314)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      909..1003
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1186..1774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1675..1694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1928..1945
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1969..2026)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    2100..2121
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 agaaatcatc cttagcgaaa gctaaggatt ttttttatct gaaattctgc ctcgtgatac
       61 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactc
      121 gagttgatcg ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
      181 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      241 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      301 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
      361 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
      421 cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata
      481 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatca
      541 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
      601 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
      661 gtatcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
      721 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
      781 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
      841 cttaatgaat tacaacagta ctgcgacgag tggcagggcg gggcgtaatt tgatatcgag
      901 ctcgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt
      961 cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagcc tcgagctgtc
     1021 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1081 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1141 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1201 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1261 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1321 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1381 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1441 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1501 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1561 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1621 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1681 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1741 gtgatgctcg tcaggggggc ggagcctgtg gaaaaacgcc agcaacgcgg cctttttacg
     1801 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1861 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     1921 cgagcgcagc gagtcagtga gcgaggaagc ctgcataacg cgaagtaatc ttttcggttt
     1981 taaagaaaaa gggcagggtg gtgacacctt gccctttttt gccggactgc agcggccgct
     2041 actagtcgcg atgagtggtc tcagtctcgt ctccgtcttt aagccgagac cactcatcgc
     2101 tctagaagcg gccgcgaatt cc
//
