LOCUS       sequence-471536-        3127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Universal acceptor vector with sfGFP placeholder for lvl0 assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_UAV_sfGFP
FEATURES             Location/Qualifiers
     source          1..3127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(524..618)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(639..1298)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1213..1232
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1460..1478
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1482..1525)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1528..1549
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             1720..1731
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1738..2451
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2463..2534
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2550..2577
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      2628..2685
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2709..2726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2880..3127,1..341))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2960..2979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
       61 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      121 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      181 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      241 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      301 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      361 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      421 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
      481 aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa
      541 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
      601 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactcg
      661 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
      721 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
      781 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
      841 aaactcaccc agggattggc tgatacgaaa aacatattct caataaaccc tttagggaaa
      901 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
      961 aaatcgtcgt ggtattcact ccagagtgat gaaaacgttt cagtttgctc atggaaaacg
     1021 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     1081 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     1141 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     1201 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     1261 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     1321 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     1381 gaacctctta cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat
     1441 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
     1501 cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagagc gatgagtggt
     1561 ctcgtgagac ggaaagtgaa acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt
     1621 atttaataat gtgtgcggca attcacattt aatttatgaa tgttttctta acatcgcggc
     1681 aactcaagaa acggcaggtt cggatcttag ctactagaga aagaggagaa atactagatg
     1741 cgtaaaggcg aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat
     1801 gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa
     1861 ctgacgctga agttcatctg tactactggt aaactgccgg ttccttggcc gactctggta
     1921 acgacgctga cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat
     1981 gacttcttca agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag
     2041 gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac
     2101 cgcattgagc tgaaaggcat tgactttaaa gaggacggca atatcctggg ccataagctg
     2161 gaatacaatt ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt
     2221 aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac
     2281 taccagcaaa acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg
     2341 agcacgcaaa gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg
     2401 gagttcgtaa ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgaccaggc
     2461 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     2521 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     2581 atacgtctca tgagaccact catcgcgact agtagcggcc gctgcagtcc ggcaaaaaag
     2641 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
     2701 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2761 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2821 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2881 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2941 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3001 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3061 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3121 aagctgg
//
