LOCUS       sequence-471537-        3135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Universal acceptor vector with sfGFP placeholder PaqCI compatible 
            for lvl0 assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_E_007_UAV_sfGFP_PaqCI
FEATURES             Location/Qualifiers
     source          1..3135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      415..509
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      692..1280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1181..1200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1434..1451
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1475..1532)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(1587..1614)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(1630..1701)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1713..2426)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             2433..2444
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    2619..2640
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2643..2686
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2690..2708)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(2870..3135,1..394)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2936..2955)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcactct ggagtgaata
       61 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
      121 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtat cagccaatcc
      181 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
      241 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
      301 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
      361 acagtactgc gacgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc
      421 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
      481 gccgccgggc gttttttatt ggtgagaatc caagcctcga gctgtcagac caagtttact
      541 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
      601 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      661 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      721 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      781 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      841 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      901 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      961 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1021 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1081 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1141 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1201 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1261 gggggcggag cctgtggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1321 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1381 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1441 cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa gaaaaagggc
     1501 agggtggtga caccttgccc ttttttgccg gactgcagcg gccgctacta gtcgcgatga
     1561 gtggtctcta tatgcaggtg tataaacgca gaaaggccca cccgaaggtg agccagtgtg
     1621 actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
     1681 ctgagccttt cgttttattt gatgcctggt catttgtaca gttcatccat accatgcgtg
     1741 atgcccgctg cggttacgaa ctccagcaga accatatgat cgcgtttctc gttcggatct
     1801 ttagacagaa cgctttgcgt gctcagatag tgattgtctg gcagcagaac aggaccatca
     1861 ccgattggag tgttttgctg gtagtgatca gccagctgca cgctgccatc ctccacgttg
     1921 tggcgaattt taaaattcgc tttaatgcca tttttttgtt tatcggcggt gatgtaaaca
     1981 ttgtggctgt taaaattgta ttccagctta tggcccagga tattgccgtc ctctttaaag
     2041 tcaatgcctt tcagctcaat gcggtttacc agggtatcgc cttcaaattt cacttccgca
     2101 cgcgttttgt acgtgccgtc atccttaaag gaaatcgtgc gttcctgcac atagccttcc
     2161 ggcatggcgg acttgaagaa gtcatgctgc ttcatatggt ccggataacg agcaaagcac
     2221 tgaacaccat aagtcagcgt cgttaccaga gtcggccaag gaaccggcag tttaccagta
     2281 gtacagatga acttcagcgt cagtttacca ttagttgcgt caccttcacc ctcgccacgc
     2341 acggaaaact tatgaccgtt gacatcacca tccagttcca ccagaatagg gacgacacca
     2401 gtgaacagct cttcgccttt acgcatctag tatttctcct ctttctctag tagctaagat
     2461 ccgaacctgc cgtttcttga gttgccgcga tgttaagaaa acattcataa attaaatgtg
     2521 aattgccgca cacattatta aataagattt acaaaatgtt caaaatgacg catgaaatca
     2581 cgtttcactt tccacctgca tatcgagacc actcatcgct ctagaagcgg ccgcgaattc
     2641 cagaaatcat ccttagcgaa agctaaggat tttttttatc tgaaattctg cctcgtgata
     2701 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2761 cgagttgatc gggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
     2821 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     2881 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     2941 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     3001 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     3061 ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat
     3121 atgggatagt gttca
//
