LOCUS       sequence-471543-        5969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes the human vasopressin receptor 1b, without any tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Dekan Z, Kremsmayr T, Keov P, Godin M, Teakle N, Durrauer L, Xiang 
            H, Gharib D, Bergmayr C, Hellinger R, Gay M, Vilaseca M, Kurzbach D,
            Albericio F, Alewood PF, Gruber CW, Muttenthaler M
  TITLE     Nature-inspired dimerization as a strategy to modulate neuropeptide 
            pharmacology exemplified with vasopressin and oxytocin.
  JOURNAL   Chem Sci. 2021 Feb 4;12(11):4057-4062. doi: 10.1039/d0sc05501h.
  PUBMED    34163676
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Sci"; date: "2021-02-4- 4"; volume: "12"; issue: "11"; pages: 
            "4057-4062"
COMMENT     Sequence Label: V1bR untagged
FEATURES             Location/Qualifiers
     source          1..5969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1909..1918
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1915..1917,1918..1920,1921..2634)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1915..1917,1918..1920,1921..2631)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1960..1981)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2221..2240)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2568..2589
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2757..2878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2794..2813)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2848..2867
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2885..3340)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3022..3043)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3234..3253
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3367..3471
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3473..3830
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3473..3664
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3496..3516)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3681..3816
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3865..4659
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3865..4659
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3919..3938)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4529..4548
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4847..4866)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4891..4938
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5267..5855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5756..5775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct catggattct gggcctctgt gggatgccaa ccccacccct
      661 cggggcaccc tctctgcccc caatgccaca acaccctggc tgggccggga tgaggagctg
      721 gccaaggtgg agatcggagt cctggccact gtcctggtgc tggcgaccgg gggcaacctg
      781 gctgtgctgc tgaccctggg ccagctgggc cgcaagcgct cccgcatgca cctgttcgtg
      841 ctgcacttag ccctgacaga cctggccgtg gcgctcttcc aggtgctgcc acagctgctg
      901 tgggacatca cctaccgctt ccagggcccc gacctcctgt gcagggccgt caagtacctg
      961 caggtgctca gcatgtttgc ctccacctac atgctgctgg ccatgacgct ggaccgctac
     1021 ctggctgtct gtcaccccct gcgcagcctc cagcagccag gccagtccac ctacctgctc
     1081 atcgctgctc cctggctgct ggccgccatc ttcagcctcc ctcaagtctt cattttttcc
     1141 ctgcgggagg tgatccaggg ctcaggggtg ctggactgct gggcagactt cggcttccct
     1201 tgggggccac gggcctacct cacctggacc accctggcta tcttcgttct gccggtgacc
     1261 atgctcacgg cctgctacag cctcatctgc catgagatct gtaaaaacct aaaagtcaag
     1321 acacaggcct ggcgggtggg aggagggggc tggaggactt gggacaggcc ctcaccttcc
     1381 accttagctg ccaccactcg ggggctgcca tctcgggtca gcagcatcaa caccatctca
     1441 cgggccaaga tccgaacagt gaagatgacc tttgtcatcg tgctggccta catcgcttgc
     1501 tgggctccct tcttcagtgt ccagatgtgg tccgtgtggg acaagaatgc ccctgatgaa
     1561 gattccacca atgtggcttt caccatctct atgcttttgg gcaacctcaa cagctgctgc
     1621 aacccctgga tctacatggg cttcaacagc cacctgttac cgcggcccct gcgtcacctt
     1681 gcctgctgtg ggggtcccca gcccaggatg cgccggcggc tctccgacgg cagcctctcg
     1741 agccgccaca ccacgctgct gacccgctcc agctgcccgg ccaccctcag cctcagcctc
     1801 agcctaaccc tcagtgggag gcccaggcct gaagagtcac caagggactt ggagctggca
     1861 gatggggaag gcaccgctga gaccatcatc ttgtaggatc caccggtcgc caccatggtg
     1921 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1981 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     2041 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2101 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     2161 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     2221 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2281 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2341 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     2401 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     2461 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2521 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2581 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     2641 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     2701 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     2761 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     2821 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
     2881 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     2941 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     3001 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3061 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3121 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3181 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3241 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3301 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     3361 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3421 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     3481 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3541 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3601 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3661 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3721 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3781 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     3841 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     3901 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     3961 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4021 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     4081 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4141 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4201 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4261 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4321 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4381 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4441 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4501 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4561 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4621 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     4681 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     4741 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     4801 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     4861 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     4921 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     4981 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     5041 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     5101 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5401 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5881 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5941 ctgtggataa ccgtattacc gccatgcat
//
