LOCUS       sequence-47156-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2289
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             79..144
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             151..171
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actgagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcaccatgga ctacaaagac catgacggtg attataaaga tcatgacatc
      121 gattacaagg atgacgatga caagatggcc cccaagaaga agaggaaggt gggcattcac
      181 cgcggggtac ctatggtgga cttgaggaca ctcggttatt cgcaacagca acaggagaaa
      241 atcaagccta aggtcaggag caccgtcgcg caacaccacg aggcgcttgt ggggcatggc
      301 ttcactcatg cgcatattgt cgcgctttca cagcaccctg cggcgcttgg gacggtggct
      361 gtcaaatacc aagatatgat tgcggccctg cccgaagcca cgcacgaggc aattgtaggg
      421 gtcggtaaac agtggtcggg agcgcgagca cttgaggcgc tgctgactgt ggcgggtgag
      481 cttagggggc ctccgctcca gctcgacacc gggcagctgc tgaagatcgc gaagagaggg
      541 ggagtaacag cggtagaggc agtgcacgcc tggcgcaatg cgctcaccgg ggcccccttg
      601 aacctgaccc cagaccaggt agtcgcaatc gcgtcacatg acgggggaaa gcaagccctg
      661 gaaaccgtgc aaaggttgtt gccggtcctt tgtcaagacc acggccttac accggagcaa
      721 gtcgtggcca ttgcatccca cgacggtggc aaacaggctc ttgagacggt tcagagactt
      781 ctcccagttc tctgtcaagc ccacgggctg actcccgatc aagttgtagc gattgcgtcg
      841 aacattggag ggaa
//
