LOCUS       sequence-471565-       10963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Translation reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 10963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-5- 5";
            volume: "165"; issue: "4"; pages: "976-89"
COMMENT     Sequence Label: pcDNA4TO-24xGCN4_v4-kif18b-24xPP7
FEATURES             Location/Qualifiers
     source          1..10963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      984..993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1059..1115
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1131..1187
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1203..1259
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1275..1331
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1347..1403
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1419..1475
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1491..1547
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1563..1619
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1635..1691
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1707..1763
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1779..1835
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1851..1907
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1923..1979
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1995..2051
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2067..2123
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2139..2195
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2211..2267
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2283..2339
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2355..2411
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2427..2483
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2499..2555
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2571..2627
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2715..2771
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(6971..6988)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6977..7201
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7247..7675
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7334..7353)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7544..7565
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7684..7704)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7689..8018
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7869..8004
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7931..7950
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8066..8113
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8132..8506
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8636..8757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8673..8692)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8727..8746
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8806..8822)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8806..8822)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8819..8841)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8830..8846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8854..8860,8861..8878,8879..8884))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8899..8920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9037..9054)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9208..9796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9288..9307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9967..10758,10759..10827))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10590..10609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10828..10932)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaatca
     1021 tcggtccggg tggatctgga ggtggaggtt ctggaggaga agaacttttg agcaagaatt
     1081 atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga gaagaactgc
     1141 tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg agcggaagtg
     1201 gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga ctaaagaaag
     1261 ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac gaagtggctc
     1321 ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac cacctcgaaa
     1381 atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta tccaagaatt
     1441 atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga gaggaactgc
     1501 tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga tcgggcagtg
     1561 gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga ttaaagaaag
     1621 gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat gaggtagcga
     1681 gactaaaaaa ggggagtggt tctggcgaag agttgctatc gaaaaattat catcttgaga
     1741 acgaagttgc taggctcaaa aagggctcag gctcaggcga ggagttgctc tcgaaaaact
     1801 accacttgga aaatgaggtc gcgaggttga aaaaggggag cgggtcgggc gaggagttat
     1861 tgagcaaaaa ctatcattta gagaacgaag tcgcgcgctt aaagaaaggc tcgggctcgg
     1921 gcgaagaact cttatcgaag aactaccacc tcgaaaatga ggtcgccagg ttgaaaaagg
     1981 gcagtggcag cggggaggaa ctcttgagca agaactacca cttggagaat gaggtcgcga
     2041 gattgaagaa agggtcgggg agcggcgagg aattgctcag caagaattat catttggaga
     2101 acgaagtcgc caggctcaag aaaggctcgg ggtcggggga ggaattgttg agtaaaaact
     2161 accacttgga aaatgaagtc gccaggctca aaaaagggag tgggagcggc gaagagttat
     2221 tgagcaaaaa ttaccacttg gagaacgaag tggcaaggct caagaaaggg agcggcagcg
     2281 gggaggagct cttatcgaag aactaccact tagagaatga agtcgcccgc ttgaagaaag
     2341 gctcggggag cggggaagag ctcttgagca agaactacca cttggaaaat gaggtggcgc
     2401 gcttgaagaa agggagcggg agcggggaag agttactatc taagaattat catctcgaga
     2461 acgaggtggc tcgactaaag aagggctccg gcagtgggga ggaactcctg tcgaagaact
     2521 atcatcttga aaatgaggtt gcaagactta aaaaggggtc cggatcaggt gaggaactac
     2581 tcagtaagaa ttaccacctg gaaaacgaag ttgcacgttt gaagaaagga tcaggatcag
     2641 gcgaagaact gctctcaaaa gattatcatt tggaaaatga ggttgcacgt ttaaaaaagg
     2701 gaagtggcag tggtgaggaa cttctgtcga aaaattatca tctcgagaat gaagtagccc
     2761 gacttaaaaa gggttctggc tcgggtcagc ggccgcaccg gtcagcagtg gaggacagca
     2821 cgctgcaagt agtggtacgg gtgcggcccc ccacccctcg ggagctggac agtcagcggc
     2881 ggccagtggt tcaggtggtg gacgagcggg tgctggtgtt taaccctgag gagcccgatg
     2941 gagggttccc tggcctgaaa tggggtggca cccatgatgg ccccaagaag aagggcaaag
     3001 acctgacgtt tgtctttgac cgggtctttg gcgaggcggc cacccaacag gacgtgttcc
     3061 agcacaccac gcacagcgtc ctggacagct tcctccaggg ctacaactgc tcagtgtttg
     3121 cctacggggc caccggggct gggaagacac acaccatgct gggaagggag ggggaccccg
     3181 gcatcatgta cctgaccacc gtggaactgt acaggcgcct ggaggcccgc cagcaggaga
     3241 agcacttcga ggtgctcatc agctaccagg aggtgtataa tgaacagatc catgacctcc
     3301 tggagcccaa ggggcccctt gccatccgcg aggaccccga caagggggtg gtggtgcaag
     3361 gactttcttt ccaccagcca gcctcagccg agcagctgct ggagatactg accaggggga
     3421 accgtaaccg cacgcagcac cccactgatg ccaacgcgac ttcctcccgc tcccatgcca
     3481 tcttccagat ctttgtgaag cagcaggacc gggttccagg actgacccag gctgtccagg
     3541 tggccaagat gagcctgatt gacctggctg gctcagagcg ggcatccagc acccatgcga
     3601 agggggagcg gctgcgggag ggggccaaca tcaaccgctc tctgctggcg ctcatcaacg
     3661 tcctcaatgc cttggccgat gcaaagggcc gcaagaccgc tgtgccctac gcggacagcg
     3721 cactgacccg cctgctcaaa gactccctcg ggggcaactg ccgcacagtg atgatcgctg
     3781 ccatcagccc ctccagcctg acctacgagg acacgtataa taccctcaaa tatgccgacc
     3841 gggccaagga gatcaggctc tcgctgaaga gcaatgtgac cagcctggac tgtcacatca
     3901 gccagtatgc taccatctgc caacagctcc aggctgaggt agccgctctg aggaagaagc
     3961 tccaagtgta tgagggggga ggccagcccc caccacagga cctcccagga tctcccaagt
     4021 cgggaccacc accagaacac cttcccagct cccccttgcc accccaccct cccagccagc
     4081 cctgcacccc agagctccct gcagggccta gagcccttca agaggagagt ctggggatgg
     4141 aggcccaggt ggagagggcc atggaaggga actcttcaga ccaggagcag tccccagagg
     4201 atgaggatga aggcccagct gaggaggttc caacccagat gccagagcag aaccccacac
     4261 atgcactgcc agagtcccct cgcctgaccc tgcagcccaa gccagtcgtg ggccacttct
     4321 cagcacggga actggatggg gaccgttcta agcggttggc cctaaaggtg ctgtgcgttg
     4381 cccagcggca gtactccctg ctccaagcag ccaacctcct gacgcccgac atgatcacag
     4441 agtttgagac cctacagcag ctggtgcaag aggaaaaaat tgagcctggg gcagaggcct
     4501 tgaggacttc aggcctggcc aggggggcac ctctggctca ggagctgtgt tcagagtcaa
     4561 agcctccagg atacactggc cctgtgaccc ggactatggc gaggcgactg agtggccccc
     4621 tgcacaccct gggaatcccg cctggaccca actgcacccc agcccagggg tcccgatggc
     4681 ccatggagaa gaagaggagg agaccaagcg ccttggaggc agacagtccc atggccccaa
     4741 agcggggcac caagcgccag cgccagtcct tcctgccctg cctaaggaga gggtctctgc
     4801 ctgacaccca accttcacag gggcccagca cccccaaagg agaaagggcc tcctccccct
     4861 gccattcccc tcgcgtttgc ccagccacag tcatcaaaag ccgggtgccc ctgggccctt
     4921 ccgccatgca gaactgctcc accccgctgg ctctgcccac tcgagacctc aatgccacct
     4981 ttgatctctc tgaggagcct ccctcaaagc ccagtttcca tgaatgcatt ggctgggaca
     5041 aaatacccca ggagctgagc aggctggacc agcccttcat ccccagggca cctgtgcccc
     5101 tgttcaccat gaagggcccc aagccaacat cttccctccc tgggacctct gcctgcaaga
     5161 agaagcgcgt tgcgagttcc tcagtctccc atggccgcag ccgcatcgcc cgcctcccca
     5221 gcagcacttt gaagaggcca gctgggcccc ttgtactccc agagctgccc ttgagtcccc
     5281 tgtgccctag caaccggagg aatggaaagg acctcatcag ggtggggaga gcactctcag
     5341 cagggaacgg cgtcaccaag gtgtccgata tctaacgaca cgattcgaag atcgcatcgc
     5401 tagcccggtt gtacaggtac cggatcctaa ggtacctaat tgcctagaaa ggagcagacg
     5461 atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg ggctcgctgg
     5521 ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa aggagcagac
     5581 gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg
     5641 gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga
     5701 cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct
     5761 ggctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag
     5821 acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc
     5881 tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca
     5941 gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg
     6001 ctggctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc
     6061 agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc
     6121 gctggctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag
     6181 cagacgatat ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct
     6241 cgctggctgc agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga
     6301 gcagacgata tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc
     6361 tcgctggctg cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg
     6421 agcagacgat atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg
     6481 ctcgctggct gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaag
     6541 gagcagacga tatggcgtcg ctccctgcag gtcgactcta gaaaccagca gagcatatgg
     6601 gctcgctggc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
     6661 ggagcagacg atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg
     6721 ggctcgctgg ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa
     6781 aggagcagac gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat
     6841 gggctcgctg gctgcagtat tcccgggttc attagatctc gcgaagggcg aattcacgag
     6901 gcgcgcccgg atcgcgatcg caatcttaat taactcgagt ctagagggcc cgtttaaacc
     6961 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7021 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7081 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7141 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     7201 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     7261 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7321 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7381 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7441 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7501 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7561 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7621 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     7681 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     7741 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     7801 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     7861 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7921 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     7981 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     8041 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     8101 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     8161 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     8221 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     8281 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     8341 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     8401 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     8461 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     8521 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     8581 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     8641 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8701 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8761 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     8821 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8881 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8941 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     9001 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9061 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9121 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9841 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    10021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    10081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    10141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    10201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    10261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10741 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10801 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10861 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10921 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
