LOCUS       sequence-471573-       14713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for PaqCI Golden Gate assembly of ISDra2 guides into 
            ISDra2 plant expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14713)
  AUTHORS   Weiss T, Kamalu M, Shi H, Li Z, Amerasekera J, Zhong Z, Adler BA, 
            Song MM, Vohra K, Wirnowski G, Chitkara S, Ambrose C, Steinmetz N, 
            Sridharan A, Sahagun D, Banfield JF, Doudna JA, Jacobsen SE
  TITLE     Viral delivery of an RNA-guided genome editor for transgene-free 
            germline editing in Arabidopsis.
  JOURNAL   Nat Plants. 2025 Apr 22. doi: 10.1038/s41477-025-01989-9.
  PUBMED    40263581
REFERENCE   2  (bases 1 to 14713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2025 Apr 22. doi: 10.1038/s41477-025-01989-9."
COMMENT     Sequence Label: pMK003
FEATURES             Location/Qualifiers
     source          1..14713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             442..1071
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1500..2573
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2639..2833
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3069..3091)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3177..3317
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3232..3251
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3332..3349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3503..4091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3583..3602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4178..4972)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    5397..5421
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    5499..5673
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5714..6739)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6806..7482)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6849..6869)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    7673..7694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7709..7714,7715..7732,7733..7739)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7747..7763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7752..7774
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7771..7787
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7771..7787
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     ncRNA           complement(8438..8505)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     protein_bind    8517..8641
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(8682..8987)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPASVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8784..8803)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(9307..9987)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     promoter        complement(join(10041..10047,10048..10065,10066..10071))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(10096..10220)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(11490..11510)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(11517..11564)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     primer_bind     complement(13611..13628)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13611..13627)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13620..13642)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    13830..13854
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
       61 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
      121 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
      181 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
      241 cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
      301 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
      361 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
      421 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
      481 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
      541 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
      601 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
      661 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
      721 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
      781 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
      841 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
      901 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
      961 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     1021 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     1081 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     1141 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     1201 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     1261 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
     1321 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     1381 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     1441 ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     1501 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
     1561 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
     1621 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
     1681 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     1741 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     1801 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     1861 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
     1921 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     1981 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     2041 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     2101 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     2161 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     2221 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     2281 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     2341 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     2401 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
     2461 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
     2521 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
     2581 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg
     2641 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
     2701 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
     2761 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
     2821 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
     2881 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
     2941 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
     3001 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
     3061 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     3121 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     3181 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     3241 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     3301 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3361 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3421 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3481 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3541 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3601 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3661 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3721 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3781 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3841 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3901 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     3961 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4021 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4081 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4141 actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa
     4201 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
     4261 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
     4321 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
     4381 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
     4441 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt
     4501 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
     4561 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
     4621 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
     4681 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
     4741 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
     4801 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
     4861 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
     4921 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt
     4981 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
     5041 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
     5101 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg
     5161 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat
     5221 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc
     5281 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct
     5341 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc
     5401 aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg
     5461 tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt agtactggat
     5521 tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac
     5581 taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta
     5641 tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg acagatcccg
     5701 gtcggcatct actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat
     5761 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg
     5821 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag
     5881 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
     5941 gcatatacgc ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
     6001 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt
     6061 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt
     6121 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt
     6181 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
     6241 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
     6301 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
     6361 gatcggccgc agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt
     6421 cggtttcagg caggtcttgc aacgtgacac cctgtgaacg gcgggagatg caataggtca
     6481 ggctctcgct aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
     6541 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
     6601 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
     6661 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
     6721 tgagttcagg ctttttcata tctcattgcc ccccggatct gcgaaagctc gagagagata
     6781 gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt
     6841 atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg
     6901 agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
     6961 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga
     7021 tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc
     7081 ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
     7141 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg
     7201 agtagacgag agtgtcgtgc tccaccatgt tcacatcaat ccacttgctt tgaagacgtg
     7261 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
     7321 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag
     7381 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
     7441 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
     7501 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggca
     7561 agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7621 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7681 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7741 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat
     7801 tcgttgtcaa tcaattggca agtcataaaa tgcattaaaa aatattttca tactcaacta
     7861 caaatccatg agtataacta taattataaa gcaatgatta gaatctgaca aggattctgg
     7921 aaaattacat aaaggaaagt tcataaatgt ctaaaacaca agaggacata cttgtattca
     7981 gtaacatttg cagcttttct aggtctgaaa atatatttgt tgcctagtga ataagcataa
     8041 tggtacaact acaagtgttt tactcctcat attaacttcg gtcattagag gccacgattt
     8101 gacacatttt tactcaaaac aaaatgtttg catatctctt ataatttcaa attcaacaca
     8161 caacaaataa gagaaaaaac aaataatatt aatttgagaa tgaacaaaag gaccatatca
     8221 ttcattaact cttctccatc catttccatt tcacagttcg atagcgaaaa ccgaataaaa
     8281 aacacagtaa attacaagca caacaaatgg tacaagaaaa acagttttcc caatgccata
     8341 atactcaaac tcagtaggat tctggtgtgt gcgcaatgaa actgatgcat tgaacttgac
     8401 gaacgttgtc gaaaccgatg atacgaacga aagctctgtc ccattcgcca tgccgaagca
     8461 tgttgcccag ccggcgccag cgaggaggct gggaccatgc cggcccatcg caggtgacca
     8521 ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     8581 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     8641 ggtcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc
     8701 aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg
     8761 ataacggagg ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg
     8821 atatgcacca ccgggtaaag ttcacggggg actttatctg acagcagacg tgcactggcc
     8881 agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac
     8941 agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct
     9001 gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc
     9061 ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt
     9121 gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg
     9181 tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggtat
     9241 acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc
     9301 tggcttttag taagccggat cctctagatt acgccccgcc tgccactcat cgcagtactg
     9361 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     9421 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     9481 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     9541 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     9601 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     9661 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     9721 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg
     9781 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     9841 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     9901 agcgactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     9961 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
    10021 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
    10081 tcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
    10141 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
    10201 gcttttttgt acaaacttgt cacctgcgat gttgaacctc acacgactaa agtcgcgtga
    10261 ttctcacttc gcagaccatt gcccgccgaa gcaggtctga catagcctcc atgagcgttt
    10321 aaggtgtctg agattcccgc agccaccaac gcttcacgcc gaatgttcag cgcagcgttc
    10381 tcgtctcggt catgggtttc cccacagttc gggcaagtcc atgtacggac ggcaagattc
    10441 ttcacttcgg gattcttgaa tccgcagtca tgacacaact ggctagatgg aaagtagggg
    10501 ctgactttag ataccagtcg cccgtaccac gctgccttgt attccaactg ccggatgaac
    10561 tcaccccagc ccgcatcact gatgctcagt gcaaggcggc gatttttccg catgttgtcg
    10621 ggtttaaggt gttcggttcc gatgatttcg tactcacgca ccagggaggt ggtgagcttg
    10681 tgaaggaaat cctgacgctt attgacaatg cgcttgtgaa tccgagccag cttggttttc
    10741 gctttcccgt aacgtgcgct gcccttcttc cgtctggaca gggtttgctg agctttacga
    10801 agtcttttca gggtggagcg gtaatatttc ggattctgtt catgcttaaa cctcacgcca
    10861 tcggtcacga tggcaaaatc cttgatgccg acatccacac ccgctgcaaa cttgggagcc
    10921 gcaggcaggt agggaatctc gacttcacag agaacggacg cttcgtaatg gccttcgtga
    10981 atacggcgca cagtgacatt cagaatcttc ccttgaatat cctgctggcc cttggttttc
    11041 acccatccca gcttaggaag tttgagccta ccttccccaa tttggatgtt gttgttggtg
    11101 aattgagtcc ggtaggactc tcccgtgcgc ttctttctga aacgtgggaa tcctaccttt
    11161 ttaccggact gcttcacagt ccgaaagaag ttcttgtacg cggtttcaag gtttttcagc
    11221 gagttctgca aagcaaactt atctacttcc gagagccagg aggtttcttc agcctgcttc
    11281 agaagggtca gttcgctact cgtttgcccg taggtcagtc ccttcccgct ttccttgtag
    11341 gccgcaatgc gacgggcaag gaagtggttg tagacgaacc ttgcgctacc cagcgtgcgg
    11401 ttaatcagtt cagtctgagc cgcatttggg tacagcctga ccacgaaagc cttattcctt
    11461 atcatagcag ctggaactcc gtggattcca acctttctct tcttctttgg agccatctta
    11521 tcatcatcat ccttgtaatc cttatcatca tcatccttgt aatccatctg ttaatcagaa
    11581 aaactcagat taatcgacaa attcgatcgc acaaactaga aactaacacc agatctagat
    11641 agaaatcaca aatcgaagag taattattcg acaaaactca aattatttga acaaatcgga
    11701 tgatatctat gaaaccctaa tcgagaatta agatgatatc taacgatcaa acccagaaaa
    11761 tcgtcttcga tctaagatta acagaatcta aaccaaagaa catatacgaa attgggatcg
    11821 aacgaaaaca aaatcgaaga ttttgagaga ataaggaaca cagaaattta ccttgatcac
    11881 ggtagagaga attgagagaa agtttttaag attttgagaa attgaaatct gaattgtgaa
    11941 gaagaagagc tctttgggta ttgttttata gaagaagaag aagaaaagac gaggacgact
    12001 aggtcacgag aaagctaagg cggtgaagca atagctaata ataaaatgac acgtgtattg
    12061 agcgttgttt acacgcaaag ttgtttttgg ctaattgcct tatttttagg ttgaggaaaa
    12121 gtatttgtgc tttgagttga taaacacgac tcgtgtgtgc cggctgcaac cactttgacg
    12181 ccgtttatta ctgactcgtc gacaaccaca atttctaacg gtcgtcataa gatccagccg
    12241 ttgagattta acgatcgtta cgatttatat ttttttagca ttatcgtttt attttttaaa
    12301 tatacggtgg agctgaaaat tggcaataat tgaaccgtgg gtcccactgc attgaagcgt
    12361 atttcgtatt ttctagaatt cttcgtgctt tatttctttt cctttttgtt tttttttgcc
    12421 atttatctaa tgcaagtggg cttataaaat cagtgaattt cttggaaaag taacttcttt
    12481 atcgtataac atattgtgaa attatccatt tcttttaatt ttttagtgtt attggatatt
    12541 tttgtatgat tattgatttg cataggataa tgacttttgt atcaagttgg tgaacaagtc
    12601 tcgttaaaaa aggcaagtgg tttggtgact cgatttattc ttgttattta attcatatat
    12661 caatggatct tatttggggc ctggtccata tttaacactc gtgttcagtc caatgaccaa
    12721 taatattttt tcattaataa caatgtaaca agaatgatac acaaaacatt ctttgaataa
    12781 gttcgctatg aagaagggaa cttatccggt cctagatcat cagttcatac aaacctccat
    12841 agagttcaac atcttaaaca agaatatcct gatctgaaga atgtggaggc tttagtccct
    12901 tggatacttg ggaggctgtg gaagaacaga aacgagctgg tgcttaaagg gagggaattt
    12961 ggaaccaatg aggtattagt aaggacacaa gaagatgcag atgagtggat tagaaggaaa
    13021 gaggctcaga atgtaaggaa agcaccaacc acaacaccgt cgggacagac gagaaacatg
    13081 ggaagctcca ccacaatctt gggttaagtg caactttgat ggggcatggc caacagaagg
    13141 attaaaatgt ggcttagggt gggtgcttcg cgatcataca gggaaggtgt tatggttagg
    13201 tgcaagagct gtggtaaaag taagaagcgt gctggaagta gaagtggagg ctcttagatg
    13261 ggctgtgctg tcattatccc gattcaatta taggaagatc atttttgagg tggattctca
    13321 gcaacttgtg tctttggtta catgaaagtt atgcttgtca agtctcaatc caattatcca
    13381 agacataaag tatctactta gcaagtttga ggattttatg cttgtgcata caagccgaga
    13441 aggaaatgga gtggcagata gaatagctaa ggaatctctt tcttttgaga attatgatct
    13501 aaagttgtat tctattgtac caatttgggt taaaagctct gttgagctag actggtacct
    13561 tcctaggata ctagtgatct agagtcgacc tgcaggcatg caagcttggc actggccgtc
    13621 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    13681 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    13741 cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt
    13801 tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
    13861 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
    13921 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
    13981 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
    14041 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
    14101 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
    14161 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
    14221 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
    14281 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
    14341 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
    14401 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
    14461 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
    14521 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
    14581 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
    14641 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
    14701 ggcgtgcatc gct
//
