LOCUS       sequence-471647-       20179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human VPS34 complex I in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20179)
  AUTHORS   Ohashi Y, Soler N, Garcia Ortegon M, Zhang L, Kirsten ML, Perisic O,
            Masson GR, Burke JE, Jakobi AJ, Apostolakis AA, Johnson CM, Ohashi 
            M, Ktistakis NT, Sachse C, Williams RL
  TITLE     Characterization of Atg38 and NRBF2, a fifth subunit of the 
            autophagic Vps34/PIK3C3 complex.
  JOURNAL   Autophagy. 2016 Nov;12(11):2129-2144. doi: 
            10.1080/15548627.2016.1226736. Epub 2016 Sep 14.
  PUBMED    27630019
REFERENCE   2  (bases 1 to 20179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2016.1226736"; journalName: "Autophagy"; date: 
            "2016-11"; volume: "12"; issue: "11"; pages: "2129-2144"
COMMENT     Sequence Label: pYO395
FEATURES             Location/Qualifiers
     source          1..20179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        248..627
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        628..831
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    833..851
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    854..872
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1029..1049
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1065..1238
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1239..1412
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1422..1442
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1473..1493
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1515..1535
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3132..3356
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3398..3777
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3778..3981
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4026..4045
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4026..4044
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4140..4142,4143..4145,4146..4859)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4140..4142,4143..4145,4146..4856)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4185..4206)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4446..4465)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4793..4814
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4869..4887)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4870..4887)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    4913..5137
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        5179..5558
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5559..5762
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    5764..5782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5785..5803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    7330..7554
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        7596..7975
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7976..8179
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    8181..8199
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8202..8220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    11058..11282
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        11324..11703
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11704..11907
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    11909..11927
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11930..11948
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    16193..16417
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      16463..16891
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16550..16569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16760..16781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(16900..16920)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        16905..17234
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      17085..17220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     17147..17166
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        17282..17329
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             17348..17722
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    17852..17973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(17889..17908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     17943..17962
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(18022..18038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18022..18038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(18035..18057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    18046..18062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(18070..18076,18077..18094,18095..18100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18115..18136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(18253..18270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18424..19012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18504..18523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(19183..19974,19975..20043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     19806..19825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(20044..20148)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatgtacg ggccagatat
      241 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      301 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      361 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      421 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      481 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      541 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      601 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      661 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      721 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      781 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccctatc
      841 agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt
      901 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
      961 tccagcctcc ggactctagc gtttaaactt aagcttggta ccggatggga gcaggggcgg
     1021 gtgctagcga gaacctgtac ttccagggcg cccacgatga agccgtagac aacaaattca
     1081 acaaagaaca acaaaacgcg ttctatgaga tcttacattt acctaactta aacgaagaac
     1141 aacgaaacgc cttcatccaa agtttaaaag atgacccaag ccaaagcgct aaccttttag
     1201 cagaagctaa aaagctaaat gatgctcagg cgccgaaagt agacaacaaa ttcaacaaag
     1261 aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa gaacaacgaa
     1321 acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt ttagcagaag
     1381 ctaaaaagct aaatgatgct caggcgccga aagtagacgc ggaaaacctg tatttccagg
     1441 gaggcggatc ttctagacca actactgcct ctgaaaattt gtactttcaa ggcgagttga
     1501 agaccactgc ttctgaaaac ttgtacttcc aaggtgagtt actcgagggt gaattgaaga
     1561 ctgctgcttt ggctcaacac gacgaagcca tgacggcgtc tcccagtggg aagggagccc
     1621 gggcgctgga ggctcctggc tgcgggcccc ggccgctcgc ccgggacctg gtggactccg
     1681 tggacgatgc ggaggggctg tacgtggctg tggagcgctg cccgctgtgc aacactaccc
     1741 gccggcggct gacctgcgcc aaatgcgttc agagcggcga tttcgtctac ttcgacggcc
     1801 gcgaccggga gaggtttatc gacaagaagg aaaggttaag ccgacttaag agcaagcaag
     1861 aagaatttca gaaagaagtg ttaaaagcta tggaaggaaa atggataaca gatcagttga
     1921 gatggaaaat aatgtcctgc aagatgagga ttgaacagtt aaaacaaaca atatgtaaag
     1981 gaaatgaaga aatggagaaa aattctgaag gccttctcaa aaccaaggaa aagaatcaga
     2041 agctttacag tcgagcacaa cggcaccaag agaaaaagga gaagattcag aggcataatc
     2101 gcaaacttgg tgacctggta gaaaaaaaga ccattgactt aagaagtcat tatgagcgtc
     2161 tggcaaatct tcgacgatcc catatattag agctcacctc tgtcattttt ccaatcgagg
     2221 aagtaaagac gggtgtgaga gaccccgcag atgtgtcttc agagagtgac agtgccatga
     2281 cctccagcac tgtgagcaag cttgctgaag cccggaggac aacttacctc tcaggacgat
     2341 gggtctgtga cgatcacagc ggagacacca gcattagcat tacagggcct tggattagcc
     2401 tccctaacaa tggggactac tctgcctact acagctgggt ggaggagaag aaaacaaccc
     2461 aggggcctga catggagcag agtaaccctg cctacaccat cagtgctgcg ctgtgctatg
     2521 caactcagct ggtcaacatt ctgtctcata tacttgatgt aaatcttccc aaaaagctct
     2581 gcaacagtga attttgtggc gaaaatctaa gcaagcagaa atttactcga gcagtgaaga
     2641 aactgaatgc aaatattctt tacctttgtt tttctcagca tgtaaattta gatcaattac
     2701 aaccactgca taccctcagg aatctaatgt acctggtcag tccaagctct gaacacctag
     2761 gcaggtcagg gccctttgaa gtacgagcag accttgagga gtccatggaa tttgtggatc
     2821 ccggagttgc tggagaatca gatgagagcg gagatgagcg cgtcagcgat gaagaaaccg
     2881 acctgggcac agactgggag aacttgccta gtccccggtt ttgtgatatc ccttcccagt
     2941 ctgtggaagt ctcccagagt cagagcaccc aggcgtcccc acccatcgcg agcagcagtg
     3001 caggtgggat gatctcctct gcagcagcct cggtgacctc ctggtttaaa gcttacactg
     3061 gacaccgtag atcctaaccc aagcttaagt cgagtctaga gggcccgttt aaacccgctg
     3121 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3181 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3241 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3301 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3361 tgaggcggaa agaaccagct ggggctctag gcgcgttgac attgattatt gactagttat
     3421 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     3481 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     3541 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     3601 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     3661 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     3721 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     3781 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     3841 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     3901 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     3961 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
     4021 gaaattaata cgactcacta tagggagacc caagcttggt accgagctcg gatccactag
     4081 taacggccgc cagtgtgctg gaattctgca gatatccatc acactggcgg ccgctcgaga
     4141 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4201 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     4261 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4321 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     4381 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4441 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4501 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4561 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     4621 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4681 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4741 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4801 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     4861 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
     4921 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4981 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5041 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5101 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     5161 tggggctcta ggcgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     5221 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     5281 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     5341 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     5401 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     5461 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     5521 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     5581 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     5641 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     5701 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     5761 ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt
     5821 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     5881 accgggaccg atccagcctc cggactctag cgtttaaact taagcttggt accatggaag
     5941 ggtctaagac gtccaacaac agcaccatgc aggtgagctt cgtgtgccag cgctgcagcc
     6001 agcccctgaa actggacacg agtttcaaga tcctggaccg tgtcaccatc caggaactca
     6061 cagctccatt acttaccaca gcccaggcga aaccaggaga gacccaggag gaagagacta
     6121 actcaggaga ggagccattt attgaaactc ctcgccagga tggtgtctct cgcagattca
     6181 tccccccagc caggatgatg tccacagaaa gtgccaacag cttcactctg attggggagg
     6241 catctgatgg cggcaccatg gagaacctca gccgaagact gaaggtcact ggggaccttt
     6301 ttgacatcat gtcgggccag acagatgtgg atcacccact ctgtgaggaa tgcacagata
     6361 ctcttttaga ccagctggac actcagctca acgtcactga aaatgagtgt cagaactaca
     6421 aacgctgttt ggagatctta gagcaaatga atgaggatga cagtgaacag ttacagatgg
     6481 agctaaagga gctggcacta gaggaggaga ggctgatcca ggagctggaa gacgtggaaa
     6541 agaaccgcaa gatagtggca gaaaatctcg agaaggtcca ggctgaggct gagagactgg
     6601 atcaggagga agctcagtat cagagagaat acagtgaatt taaacgacag cagctggagc
     6661 tggatgatga gctgaagagt gttgaaaacc agatgcgtta tgcccagacg cagctggata
     6721 agctgaagaa aaccaacgtc tttaatgcaa ccttccacat ctggcacagt ggacagtttg
     6781 gcacaatcaa taacttcagg ctgggtcgcc tgcccagtgt tcccgtggaa tggaatgaga
     6841 ttaatgctgc ttggggccag actgtgttgc tgctccatgc tctggccaat aagatgggtc
     6901 tgaaatttca gagataccga cttgttcctt acggaaacca ttcatatctg gagtctctga
     6961 cagacaaatc taaggagctg ccgttatact gttctggggg gttgcggttt ttctgggaca
     7021 acaagtttga ccatgcaatg gtggctttcc tggactgtgt gcagcagttc aaagaagagg
     7081 ttgagaaagg cgagacacgt ttttgtcttc cctacaggat ggatgtggag aaaggcaaga
     7141 ttgaagacac aggaggcagt ggcggctcct attccatcaa aacccagttt aactctgagg
     7201 agcagtggac aaaagctctc aagttcatgc tgacgaatct gaagtggggt ctggcttggg
     7261 tgtcctctca attttataac aaatgagtcg agtctagagg gcccgtttaa acccgctgat
     7321 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     7381 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     7441 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     7501 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     7561 aggcggaaag aaccagctgg ggctctaggc gcgttgacat tgattattga ctagttatta
     7621 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     7681 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     7741 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     7801 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     7861 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     7921 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     7981 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     8041 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     8101 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     8161 ggtctatata agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga
     8221 tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     8281 tgacctccat agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa
     8341 gcttggtacc atgggggaag cagagaagtt tcactacatc tatagttgtg acctggatat
     8401 caacgtccag cttaagatag gaagcttgga agggaagaga gaacaaaaga gttataaagc
     8461 tgtcctggaa gacccaatgt tgaagttctc aggactatat caagagacat gctctgatct
     8521 ttatgttact tgtcaagttt ttgcagaagg gaagcctttg gccttgccag tgagaacatc
     8581 ctacaaagca tttagtacaa gatggaactg gaatgaatgg ctgaaactac cagtaaaata
     8641 ccctgacctg cccaggaatg cccaagtggc cctcaccatc tgggatgtgt atggtcccgg
     8701 aaaagcagtg cctgtaggag gaacaacggt ttcgctcttt ggaaaatacg gcatgtttcg
     8761 ccaagggatg catgacttga aagtctggcc taatgtagaa gcagatggat cagaacccac
     8821 aaaaactcct ggcagaacaa gtagcactct ctcagaagat cagatgagcc gtcttgccaa
     8881 gctcaccaaa gctcatcgac aaggacacat ggtgaaagta gattggctgg atagattgac
     8941 atttagagaa atagaaatga taaatgagag tgaaaaacga agttctaatt tcatgtacct
     9001 gatggttgaa tttcgatgtg tcaagtgtga tgataaggaa tatggtattg tttattatga
     9061 aaaggacggt gatgaatcat ctccaatttt aacaagtttt gaattagtga aagttcctga
     9121 cccccagatg tctatggaga atttagttga gagcaaacac cacaagcttg cccggagttt
     9181 aagaagtgga ccttctgacc acgatctgaa acccaatgct gccacgagag atcagttaaa
     9241 tattattgtg agttatccac caaccaagca acttacctat gaagaacaag atcttgtttg
     9301 gaagtttaga tattatctta cgaatcaaga aaaagccttg acaaaattct tgaaatgtgt
     9361 taattgggat ctacctcaag aggccaaaca ggccttggaa cttctgggaa aatggaagcc
     9421 gatggatgta gaggactcct tggagctgtt atcctctcat tacaccaacc caactgtgag
     9481 gcgttatgct gttgcccggt tgcgacaggc cgatgatgag gatttgttga tgtacctatt
     9541 acaattggtc caggctctca aatatgaaaa ttttgatgat ataaagaatg gattggaacc
     9601 taccaagaag gatagtcaga gttcagtgtc agaaaatgtg tcaaattctg gaataaattc
     9661 tgcagaaata gatagctccc aaattataac cagccccctt ccttcagtct cttcacctcc
     9721 tcctgcatca aaaacaaaag aagttccaga tggcgaaaat ctggaacaag atctctgtac
     9781 cttcttgata tcgagagcct gcaaaaactc aacactggct aattatttat actggtatgt
     9841 gatagtggaa tgtgaagatc aagatactca gcagagagat ccaaagaccc atgagatgta
     9901 cttgaacgta atgagaagat tcagccaagc attgttgaag ggtgataagt ctgtcagagt
     9961 tatgcgttct ttgctggctg cacaacagac atttgtagat cggttggtgc atctaatgaa
    10021 ggcagtacaa cgcgaaagtg gaaatcgtaa gaaaaagaat gagagactac aggcattgct
    10081 tggagataat gaaaagatga atttgtcaga tgtggaactt atcccgttgc ctttagaacc
    10141 ccaagtgaaa attagaggaa taattccgga aacagctaca ctgtttaaaa gtgcccttat
    10201 gcctgcacag ttgtttttta agacggaaga tggaggcaaa tatccagtta tatttaagca
    10261 tggagatgat ttacgtcaag atcaacttat tcttcaaatc atttcactca tggacaagct
    10321 gttacggaaa gaaaatctgg acttgaaatt gacaccttat aaggtgttag ccaccagtac
    10381 aaaacatggc ttcatgcagt ttatccagtc agttcctgtg gctgaagttc ttgatacaga
    10441 gggaagcatt cagaactttt ttagaaaata tgcaccaagt gagaatgggc caaatgggat
    10501 tagtgctgag gtcatggaca cttacgttaa aagctgtgct ggatattgcg tgatcaccta
    10561 tatacttgga gttggagaca ggcacctgga taaccttttg ctaacaaaaa caggcaaact
    10621 cttccacata gactttggat atattctggg tcgtgatcca aagcctcttc ctccaccaat
    10681 gaagctgaat aaagaaatgg tagaaggaat ggggggcaca cagagtgagc agtaccaaga
    10741 gttccgtaaa cagtgttaca cggctttcct ccacctgcga aggtattcta atctgatttt
    10801 gaacttgttt tccttgatgg ttgatgcaaa cattccagat attgcacttg aaccagataa
    10861 aactgtgaaa aaggttcagg ataaattccg cttagacctg tcggatgaag aggctgtgca
    10921 ttacatgcag agtctgattg atgagagtgt ccatgctctt tttgctgcag tggtggaaca
    10981 gattcacaag tttgcccagt actggcgcaa atgagtcgag tctagagggc ccgtttaaac
    11041 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
    11101 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
    11161 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
    11221 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
    11281 ggcttctgag gcggaaagaa ccagctgggg ctctaggcgc gttgacattg attattgact
    11341 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
    11401 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
    11461 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
    11521 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
    11581 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
    11641 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
    11701 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
    11761 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
    11821 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
    11881 cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt
    11941 gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
    12001 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt
    12061 aaacttaagc ttatgggaaa tcagcttgct ggcattgctc cctcccagat cctttctgta
    12121 gagagttatt tttcagatat tcatgacttt gaatatgata aaagcctggg gagtactcgg
    12181 ttttttaaag ttgctcgagc caagcaccga gaaggcctgg tcgttgtgaa ggtttttgca
    12241 attcaggatc ccacattgcc tttaaccagc tataaacaag agctggagga actgaaaatc
    12301 aggcttaatt ctgcacagaa ttgtctacct ttccagaaag catcagaaaa agcatctgag
    12361 aaagcagcta tgctctttag gcagtatgtg cgagacaatc tctatgatcg catcagtacc
    12421 cgtccattct tgaataacat tgagaagcgc tggattgctt tccagatcct gacagctgtg
    12481 gaccaagcac acaaatctgg agttcgtcat ggggacatca agactgagaa tgtgatggtc
    12541 accagttgga attgggttct tctaactgat tttgccagtt ttaagcccac ttatcttcca
    12601 gaagacaacc cggcagattt caattatttc tttgacacat cacggaggag aacttgctat
    12661 attgctcctg aacgttttgt tgatggtggg atgtttgcca ctgagttaga atatatgaga
    12721 gatccttcaa ctccgcttgt agacttaaat agcaatcaga gaacaagagg agagttgaag
    12781 agagcaatgg acatcttttc agcaggttgt gtgatagctg agctttttac agaaggtgta
    12841 ccattatttg atctctctca acttttggct tatagaaatg gacatttttt ccctgaacaa
    12901 gtgctaaata aaattgaaga tcacagtatc agagaattgg taactcagat gattcaccgt
    12961 gagccagata aacgtttaga ggcagaagat tacttaaaac agcagcgtgg caatgccttt
    13021 cctgaaatat tttacacttt tcttcagccc tacatggccc agtttgccaa ggaaacgttt
    13081 ctttctgcag atgagcgtat tctggttata cggaaggatt tgggcaacat tattcacaat
    13141 ctctgtggac atgatctgcc agaaaaagcc gaaggagagc ctaaggaaaa tgggctggtt
    13201 atcttggtat ctgttataac atcctgccta cagaccctta aatactgtga ttccaaacta
    13261 gctgctttgg aactgattct tcatttggct ccaagattaa gtgttgaaat ccttttggat
    13321 cgtattactc catatctttt gcatttcagc aatgactctg ttcctagggt gagggctgaa
    13381 gccttgagga cgttgaccaa agttcttgct ctcgtcaaag aggttcctcg taatgatatc
    13441 aatatttatc cggaatacat tctgccaggc atagcccact tagcccaaga tgatgctact
    13501 atcgttagac tagcctatgc tgaaaacata gctctgctgg cagaaacagc tctgagattc
    13561 ctggaattag tacagttaaa aaatcttaat atggaaaatg accccaataa tgaagaaata
    13621 gatgaggtta cacatccaaa tggaaattat gacacagagc tgcaagcctt acatgaaatg
    13681 gtccagcaga aagttgttac tttgctaagt gaccctgaaa atattgtaaa acaaaccttg
    13741 atggaaaatg gaataacacg gctgtgtgta ttctttggac gtcagaaagc caacgatgtt
    13801 ttgttgtccc acatgattac tttcctaaat gataagaatg attggcatct acgtggagca
    13861 ttttttgata gtatagttgg tgttgctgcc tatgttggct ggcaaagctc ctcaattctc
    13921 aagcctctgc tgcaacaagg tcttagtgat gctgaggaat ttgtcattgt gaaagctctt
    13981 tatgccctta cttgtatgtg ccagttagga ctgctacaaa aaccccatgt ttacgaattt
    14041 gccagtgata ttgccccctt cctgtgtcat cccaatttat ggatacgtta tggtgccgtg
    14101 ggatttatca cagtggtagc tcgtcaaata agtacagctg atgtctactg taaactgatg
    14161 ccttatcttg acccatatat tacccaacca ataatacaga ttgaaagaaa acttgttctg
    14221 ctcagtgttt taaaggaacc agtaagtcgt tctatatttg attatgcttt gaggtctaaa
    14281 gatattacta gcttgttcag acatcttcac atgcgtcaga agaaacgaaa tggttctctt
    14341 cccgactgcc ctccgccaga ggatcctgcc atagcacagc ttctgaagaa gttgctctca
    14401 cagggaatga cagaggaaga ggaagacaaa cttctggcac tgaaagactt catgatgaaa
    14461 tctaataaag caaaggccaa tatagtggac cagagccatc ttcatgatag tagtcagaaa
    14521 ggtgtaattg acttggcagc tttaggcata actgggagac aagttgatct tgttaaaacc
    14581 aaacaagaac cagatgacaa acgggccaga aaacatgtaa aacaagactc aaatgtaaat
    14641 gaagaatgga aaagcatgtt tgggtcactg gacccaccaa acatgccaca ggccctacct
    14701 aaagggagtg atcaggaggt gattcagact gggaaacctc ctcgttccga gtcctctgct
    14761 ggcatttgtg tccctttgtc aacttcttca caggttccag aagtgacaac tgtccaaaat
    14821 aaaaaaccag taataccggt tttaagtagt acaatcttac catccaccta tcagattcga
    14881 attacaactt gtaaaactga acttcagcaa ctcatccagc aaaagcggga gcagtgcaat
    14941 gctgagagaa tagctaagca gatgatggaa aatgctgaat gggagagtaa accaccacca
    15001 cctggatggc gtcctaaagg gctgttagtt gcccatcttc atgagcataa atctgctgtg
    15061 aatcgaatta gagtctctga tgaacactca ctttttgcaa catgttcaaa tgatggcaca
    15121 gtgaaaatct ggaacagtca aaagatggag gggaagacca ccactaccag atctattctt
    15181 acatacagcc gaattggagg acgagtcaag acgctcacat tctgccaagg ctcccactat
    15241 ttagccatag catctgataa tggtgctgtc cagcttcttg gaattgaggc ttctaagctg
    15301 cccaagtctc ctaaaatcca tcctctacaa agcagaattc tagatcagaa ggaggacggt
    15361 tgtgttgtgg atatgcatca cttcaactct ggagcacagt ctgttcttgc ctatgccact
    15421 gtgaatggct ctctggttgg ctgggacctt aggtcttcaa gcaatgcgtg gactttaaag
    15481 catgatttaa agtcgggcct catcacttcc tttgctgtgg acatccacca atgctggctc
    15541 tgcattggta caagcagtgg caccatggct tgttgggaca tgaggttcca gttgccaatt
    15601 tcaagtcact gtcatccttc cagggctcga atcagacgcc tctcaatgca ccctctgtat
    15661 cagtcctggg tgattgcagc tgttcagggc aacaacgaag tgtccatgtg ggacatggag
    15721 actggtgaca gaagatttac tctctgggcc agcagtgcac caccactttc tgaattacag
    15781 ccttctcctc atagcgtcca tggtatctac tgtagtcctg cagatggaaa tcctatccta
    15841 ctaacagctg gctcagatat gaaaataagg ttttgggact tggcttaccc agaaaggtcc
    15901 tatgttgttg caggaagtac tagttcccca tctgtgtcct actacaggaa aataattgaa
    15961 ggcactgaag ttgtccagga aattcagaat aagcagaaag taggaccaag tgatgacacc
    16021 cctcgaaggg gcccagagtc cctgcccgtg ggacatcatg acatcatcac tgatgtcgcc
    16081 acattccaga ccacacaggg cttcatcgta actgcttcta gagatgggat tgtgaaggtg
    16141 tggaaataag tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
    16201 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
    16261 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
    16321 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
    16381 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
    16441 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    16501 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    16561 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    16621 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    16681 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    16741 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    16801 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    16861 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
    16921 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    16981 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    17041 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
    17101 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    17161 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    17221 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
    17281 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
    17341 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
    17401 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
    17461 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
    17521 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
    17581 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
    17641 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
    17701 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
    17761 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
    17821 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
    17881 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    17941 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
    18001 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    18061 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    18121 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    18181 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    18241 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    18301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    18361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    18421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    18481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    18541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    18601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    18661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    18721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    18781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    18841 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    18901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    18961 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    19021 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    19081 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    19141 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    19201 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    19261 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    19321 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    19381 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    19441 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    19501 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    19561 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    19621 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    19681 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    19741 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    19801 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    19861 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    19921 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    19981 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    20041 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    20101 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    20161 aaaagtgcca cctgacgtc
//
