LOCUS       sequence-471656-        2070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRBV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2070)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRBV20-1
FEATURES             Location/Qualifiers
     source          1..2070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(398..414)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(398..414)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      453..484
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        485..587
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             588..1247
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(654..673)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1271..1300
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      complement(1312..1899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1392..1411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1921..1950)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     2023..2040
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2024..2040
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcacca tgctgctgct tctgctgctt ctggggccag gtataagcct ccttctacct
       61 gggagcttgg caggctccgg gcttggtgct gttgtctctc aacatccgag ctgggttatc
      121 tgtaagagtg gaacctctgt gaagatcgag tgccgttccc tggactttca ggccacaact
      181 atgttttggt atcgtcagtt cccgaaacag agtctcatgc tgatggcaac ttccaatgag
      241 ggctccaagg ccacatacga gcaaggcgtc gagaaggaca agtttctcat caaccatgca
      301 agcctgacct tgtccactct gacagtgacc agtgcccatc ctgaagacag cagcttctac
      361 atctgtgaga cgcagggaag atggccgcga agaaaacgtc atagctgttt cctgactgac
      421 catttaaatc atacctgacc tccatagcag aaagtcaaaa gcctccgacc ggaggctttt
      481 gacttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
      541 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
      601 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      661 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
      721 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
      781 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
      841 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
      901 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
      961 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgttttttg
     1021 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1081 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     1141 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     1201 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaatga gggcccaaat
     1261 gtaatcacct ggctcacctt cacgggtggg cctttctgcg ttgctggcgt ttttccatag
     1321 gctccgcccc cctgacgagc atcacaaaaa tcgatgctca agtcagaggt ggcgaaaccc
     1381 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1441 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1501 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1561 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1621 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1681 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1741 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta cctcggaaaa
     1801 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1861 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gattttctac
     1921 cgaagaaagg cccacccgtg aaggtgagcc agtgagttga ttgcagtcca gttacgctgg
     1981 agtctgaggc tcgtcctgaa tgatatcaag cttgaattcg tttgtaaaac gacggccagt
     2041 gctaaccgta tcgcattggc catacctaca
//
