LOCUS       sequence-471666-        7154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the USP7 UBL1-3 domains with an N-terminal 
            Myc tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Ashton NW, Valles GJ, Jaiswal N, Bezsonova I, Woodgate R
  TITLE     DNA Polymerase iota Interacts with Both the TRAF-like and UBL1-2 
            Domains of USP7.
  JOURNAL   J Mol Biol. 2021 Jan 22;433(2):166733. doi: 
            10.1016/j.jmb.2020.166733. Epub 2020 Dec 3.
  PUBMED    33279577
REFERENCE   2  (bases 1 to 7154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2021-01-22"; volume: "433(2):166733. doi"; pages: " 
            10.1016/j.jmb.2020.166733. Epub 2020 Dec 3"
COMMENT     Sequence Label: pcDNA3.1-N-Myc_535-888 USP7
FEATURES             Location/Qualifiers
     source          1..7154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     150..169
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(388..492)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(567..586)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        758..1137
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1138..1341
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1292..1312
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1386..1405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1386..1404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1440..1449
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1449..1478
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3268..3285)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3274..3498
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3544..3972
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3631..3650)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3841..3862
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3981..4001)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3986..4315
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4166..4301
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4228..4247
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4382..5176
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4436..4455)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5046..5065
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5350..5471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5387..5406)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5441..5460
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5520..5536)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5520..5536)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5533..5555)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5544..5560
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5568..5574,5575..5592,5593..5598))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5613..5634
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5751..5768)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5922..6510)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6002..6021)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6681..7154,1..318,319..387))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
       61 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
      121 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      181 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      241 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      301 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      361 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      421 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      481 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
      541 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
      601 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
      661 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
      721 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
      781 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      841 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      901 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      961 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     1021 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     1081 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     1141 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     1201 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     1261 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     1321 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
     1381 gaaattaata cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg
     1441 ccaccatgga gcagaaactc atctctgaag aggatctggg taccgatatt cctcagcagt
     1501 tggtggagcg attacaagaa gagaaaagga tcgaggctca gaagcggaag gagcggcagg
     1561 aagcccatct ctatatgcaa gtgcagatag tcgcagagga ccagttttgt ggccaccaag
     1621 ggaatgacat gtacgatgaa gaaaaagtga aatacactgt gttcaaagta ttgaagaact
     1681 cctcgcttgc tgagtttgtt cagagcctct ctcagaccat gggatttcca caagatcaaa
     1741 ttcgattgtg gcccatgcaa gcaaggagta atggaacaaa acgaccagca atgttagata
     1801 atgaagccga cggcaataaa acaatgattg agctcagtga taatgaaaac ccttggacaa
     1861 tattcctgga aacagttgat cccgagctgg ctgctagtgg agcgacctta cccaagtttg
     1921 ataaagatca tgatgtaatg ttatttttga agatgtatga tcccaaaacg cggagcttga
     1981 attactgtgg gcatatctac acaccaatat cctgtaaaat acgtgacttg ctcccagtta
     2041 tgtgtgacag agcaggattt attcaagata ctagccttat cctctatgag gaagttaaac
     2101 cgaatttaac agagagaatt caggactatg acgtgtctct tgataaagcc cttgatgaac
     2161 taatggatgg tgacatcata gtatttcaga aggatgaccc tgaaaatgat aacagtgaat
     2221 tacccaccgc aaaggagtat ttccgagatc tctaccaccg cgttgatgtc attttctgtg
     2281 ataaaacaat ccctaatgat cctggatttg tggttacgtt atcaaataga atgaattatt
     2341 ttcaggttgc aaagacagtt gcacagaggc tcaacacaga tccaatgttg ctgcagtttt
     2401 tcaagtctca aggttatagg gatggcccag gtaatcctct tagacataat tatgaaggta
     2461 ctttaagaga tcttctacag ttcttcaagc ctagacaacc taagaaactt tactatcagc
     2521 agcttaagat gaaaatcaca gacttttgag gatcccgaag ttttaaatgt atatggttaa
     2581 acagccaatt tagggaagag gaaataacac tatatccaga caagcatggg tgtgtccggg
     2641 acctgttaga agaatgtaaa aaggccgtgg agcttgggga gaaagcatca gggaaactta
     2701 ggctgctaga aattgtaagc tacaaaatca ttggtgttca tcaagaagat gaactattag
     2761 aatgtttatc tcctgcaacg agccggacgt ttcgaataga ggaaatccct ttggaccagg
     2821 tggacataga caaagagaat gagatgcttg tcacagtggc gcatttccac aaagaggtct
     2881 tcggaacgtt cggaatcccg tttttgctga ggatacacca gggcgagcat tttcgagaag
     2941 tgatgaagcg aatccagagc ctgctggaca tccaggagaa ggagtttgag aagtttaaat
     3001 ttgcaattgt aatgatgggc cgacaccagt acataaatga agacgagtat gaagtaaatt
     3061 tgaaagactt tgagccacag cccggtaata tgtctcatcc tcggccttgg ctagggctcg
     3121 accacttcaa caaagcccca aagaggagtc gctacactta ccttgaaaag gccattaaaa
     3181 tccataactg aggatccgaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta
     3241 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     3301 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     3361 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     3421 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     3481 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggcttt agggggtatc
     3541 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3601 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3661 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3721 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3781 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3841 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3901 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3961 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4021 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4081 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4141 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4201 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4261 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4321 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     4381 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4441 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4501 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4561 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4621 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4681 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4741 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4801 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4861 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     4921 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4981 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5041 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5101 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5161 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5221 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5281 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5341 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5401 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5461 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     5521 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5581 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5641 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5701 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5761 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5821 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5881 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5941 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6001 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6061 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6601 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6661 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6721 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6781 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6841 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6901 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6961 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7021 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7081 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7141 ttggccgcag tgtt
//
