LOCUS       sequence-471667-        8705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of N-terminally Myc-tagged USP7, minus the 
            C-terminal 18 amino acids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Ashton NW, Valles GJ, Jaiswal N, Bezsonova I, Woodgate R
  TITLE     DNA Polymerase iota Interacts with Both the TRAF-like and UBL1-2 
            Domains of USP7.
  JOURNAL   J Mol Biol. 2021 Jan 22;433(2):166733. doi: 
            10.1016/j.jmb.2020.166733. Epub 2020 Dec 3.
  PUBMED    33279577
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2021-01-22"; volume: "433(2):166733. doi"; pages: " 
            10.1016/j.jmb.2020.166733. Epub 2020 Dec 3"
COMMENT     Sequence Label: pcDNA3.1-N-Myc_1-1083 USP7
FEATURES             Location/Qualifiers
     source          1..8705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        361..564
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     515..535
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..628
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        609..627
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      663..672
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             672..701
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4042..4059)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4048..4272
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4318..4746
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4405..4424)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4615..4636
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4755..4775)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4760..5089
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4940..5075
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5002..5021
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5156..5950
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5210..5229)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5820..5839
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6124..6245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6161..6180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6215..6234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6294..6310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6294..6310)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6307..6329)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6318..6334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6342..6348,6349..6366,6367..6372))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6387..6408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6525..6542)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6696..7284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6776..6795)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7455..8246,8247..8315))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8078..8097
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8316..8420)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8495..8514)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8686..8705,1..360)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
       61 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      121 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      181 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      241 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      301 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      361 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      421 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      481 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      541 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      601 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc
      661 ttgccaccat ggagcagaaa ctcatctctg aagaggatct gggtaccatg aaccaccagc
      721 agcagcagca gcagcagaaa gcgggcgagc agcagttgag cgagcccgag gacatggaga
      781 tggaagcggg agatacagat gacccaccaa gaattactca gaaccctgtg atcaatggga
      841 atgtggccct gagtgatgga cacaacaccg cggaggagga catggaggat gacaccagtt
      901 ggcgctccga ggcaaccttt cagttcactg tggagcgctt cagcagactg agtgagtcgg
      961 tccttagccc tccgtgtttt gtgcgaaatc tgccatggaa gattatggtg atgccacgct
     1021 tttatccaga cagaccacac caaaaaagcg taggattctt tctccagtgc aatgctgaat
     1081 ctgattccac gtcatggtct tgccatgcac aagcagtgct gaagataata aattacagag
     1141 atgatgaaaa gtcgttcagt cgtcgtatta gtcatttgtt cttccataaa gaaaatgatt
     1201 ggggattttc caattttatg gcctggagtg aagtgaccga tcctgagaaa ggatttatcg
     1261 atgatgacaa agttaccttt gaagtctttg tacaggcgga tgctccccat ggagttgcgt
     1321 gggattcaaa gaagcacacc ggttacgtcg gcttaaagaa tcagggagcg acttgttaca
     1381 tgaacagcct gctacagacg ttatttttca cgaatcagct acgaaaggct gtgtacatga
     1441 tgccaaccga gggggatgat tcgtctaaaa gcgtcccttt agcattacaa agagtgttct
     1501 atgaattaca gcatagtgat aaacctgtag gaacaaaaaa gttaacaaag tcatttgggt
     1561 gggaaacttt agatagcttc atgcaacatg atgttcagga gctttgtcga gtgttgctcg
     1621 ataatgtgga aaataagatg aaaggcacct gtgtagaggg caccataccc aaattattcc
     1681 gcggcaaaat ggtgtcctat atccagtgta aagaagtaga ctatcggtct gatagaagag
     1741 aagattatta tgatatccag ctaagtatca aaggaaagaa aaatatattt gaatcatttg
     1801 tggattatgt ggcagtagaa cagctcgatg gggacaataa atacgacgct ggggaacatg
     1861 gcttacagga agcagagaaa ggtgtgaaat tcctaacatt gccaccagtg ttacatctac
     1921 aactgatgag atttatgtat gaccctcaga cggaccaaaa tatcaagatc aatgataggt
     1981 ttgaattccc agagcagtta ccacttgatg aatttttgca aaaaacagat cctaaggacc
     2041 ctgcaaatta tattcttcat gcagtcctgg ttcatagtgg agataatcat ggtggacatt
     2101 atgtggttta tctaaacccc aaaggggatg gcaaatggtg taaatttgat gacgacgtgg
     2161 tgtcaaggtg tactaaagag gaagcaattg agcacaatta tgggggtcac gatgacgacc
     2221 tgtctgttcg acactgcact aatgcttaca tgttagtcta catcagggaa tcaaaactga
     2281 gtgaagtttt acaggcggtc accgaccatg atattcctca gcagttggtg gagcgattac
     2341 aagaagagaa aaggatcgag gctcagaagc ggaaggagcg gcaggaagcc catctctata
     2401 tgcaagtgca gatagtcgca gaggaccagt tttgtggcca ccaagggaat gacatgtacg
     2461 atgaagaaaa agtgaaatac actgtgttca aagtattgaa gaactcctcg cttgctgagt
     2521 ttgttcagag cctctctcag accatgggat ttccacaaga tcaaattcga ttgtggccca
     2581 tgcaagcaag gagtaatgga acaaaacgac cagcaatgtt agataatgaa gccgacggca
     2641 ataaaacaat gattgagctc agtgataatg aaaacccttg gacaatattc ctggaaacag
     2701 ttgatcccga gctggctgct agtggagcga ccttacccaa gtttgataaa gatcatgatg
     2761 taatgttatt tttgaagatg tatgatccca aaacgcggag cttgaattac tgtgggcata
     2821 tctacacacc aatatcctgt aaaatacgtg acttgctccc agttatgtgt gacagagcag
     2881 gatttattca agatactagc cttatcctct atgaggaagt taaaccgaat ttaacagaga
     2941 gaattcagga ctatgacgtg tctcttgata aagcccttga tgaactaatg gatggtgaca
     3001 tcatagtatt tcagaaggat gaccctgaaa atgataacag tgaattaccc accgcaaagg
     3061 agtatttccg agatctctac caccgcgttg atgtcatttt ctgtgataaa acaatcccta
     3121 atgatcctgg atttgtggtt acgttatcaa atagaatgaa ttattttcag gttgcaaaga
     3181 cagttgcaca gaggctcaac acagatccaa tgttgctgca gtttttcaag tctcaaggtt
     3241 atagggatgg cccaggtaat cctcttagac ataattatga aggtacttta agagatcttc
     3301 tacagttctt caagcctaga caacctaaga aactttacta tcagcagctt aagatgaaaa
     3361 tcacagactt tgagaacagg cgaagtttta aatgtatatg gttaaacagc caatttaggg
     3421 aagaggaaat aacactatat ccagacaagc atgggtgtgt ccgggacctg ttagaagaat
     3481 gtaaaaaggc cgtggagctt ggggagaaag catcagggaa acttaggctg ctagaaattg
     3541 taagctacaa aatcattggt gttcatcaag aagatgaact attagaatgt ttatctcctg
     3601 caacgagccg gacgtttcga atagaggaaa tccctttgga ccaggtggac atagacaaag
     3661 agaatgagat gcttgtcaca gtggcgcatt tccacaaaga ggtcttcgga acgttcggaa
     3721 tcccgttttt gctgaggata caccagggcg agcattttcg agaagtgatg aagcgaatcc
     3781 agagcctgct ggacatccag gagaaggagt ttgagaagtt taaatttgca attgtaatga
     3841 tgggccgaca ccagtacata aatgaagacg agtatgaagt aaatttgaaa gactttgagc
     3901 cacagcccgg taatatgtct catcctcggc cttggctagg gctcgaccac ttcaactgaa
     3961 ggcctggatc cgaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc
     4021 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4081 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4141 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4201 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4261 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4321 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4381 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4441 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4501 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4561 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4621 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4681 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4741 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4801 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4861 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4921 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4981 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     5041 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     5101 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     5161 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     5221 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     5281 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     5341 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     5401 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     5461 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     5521 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5581 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     5641 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     5701 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     5761 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     5821 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     5881 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     5941 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     6001 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6061 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6121 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6181 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6241 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6301 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6361 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6421 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6481 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6541 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6601 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6661 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6721 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6781 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6841 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6901 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6961 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7021 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7081 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7141 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7201 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7261 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7321 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7381 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7441 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7501 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7561 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7621 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7681 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7741 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7801 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7861 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7921 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7981 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8041 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8101 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8161 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8221 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8281 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8341 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8401 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     8461 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     8521 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     8581 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     8641 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     8701 ctagt
//
