LOCUS       sequence-471671-        9632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tetracycline inducible PiggyBac vector expressing human CAII gene 
            including flag-tag (DYKDDDDK) on the 3' end.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Rynes J, Istvankova E, Dzurov Krafcikova M, Luchinat E, Barbieri L, 
            Banci L, Kamarytova K, Loja T, Fafilek B, Rico-Llanos G, Krejci P, 
            Macurek L, Foldynova-Trantirkova S, Trantirek L
  TITLE     Protein structure and interactions elucidated with in-cell NMR for 
            different cell cycle phases and in 3D human tissue models.
  JOURNAL   Commun Biol. 2025 Feb 7;8(1):194. doi: 10.1038/s42003-025-07607-w.
  PUBMED    39920376
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2025-02-7"; volume: "8(1):194. doi"; pages: " 
            10.1038/s42003-025-07607-w"
COMMENT     Sequence Label: pB-TR-hCAII
FEATURES             Location/Qualifiers
     source          1..9632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1421..1724
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1725..1928
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1879..1899
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1930..1948
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1951..1969
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1979..2003
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2871..2894
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(2907..3481,3482..3484,3485..3493)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3074..3091)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3301..3320
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3530..4192
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     promoter        4313..4524
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4537..4805
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4842..5462
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4915..4934)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             5496..5549
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5556..6155
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5556..5575)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6052..6072
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6202..6323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6239..6258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6293..6312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7086..7120
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7611..7627)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7611..7627)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7624..7646)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7635..7651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7659..7665,7666..7683,7684..7689))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7704..7725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7842..7859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8013..8601)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8093..8112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8772..9563,9564..9632))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9395..9414
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagttattaa tagtaatcaa
     1381 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1441 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1501 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1561 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1621 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1681 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     1741 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     1801 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1861 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1921 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
     1981 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2041 gaagacaccg ggaccgatcc agcctccgga ctctagcggt ctaggctagc atgtcccatc
     2101 actgggggta cggcaaacac aacggacctg agcactggca taaggacttc cccattgcca
     2161 agggagagcg ccagtcccct gttgacatcg acactcatac agccaagtat gacccttccc
     2221 tgaagcccct gtctgtttcc tatgatcaag caacttccct gaggatcctc aacaatggtc
     2281 atgctttcaa cgtggagttt gatgactctc aggacaaagc agtgctcaag ggaggacccc
     2341 tggatggcac ttacagattg attcagtttc actttcactg gggttcactt gatggacaag
     2401 gttcagagca tactgtggat aaaaagaaat atgctgcaga acttcacttg gttcactgga
     2461 acaccaaata tggggatttt gggaaagctg tgcagcaacc tgatggactg gccgttctag
     2521 gtattttttt gaaggttggc agcgctaaac cgggccttca gaaagttgtt gatgtgctgg
     2581 attccattaa aacaaagggc aagagtgctg acttcactaa cttcgatcct cgtggcctcc
     2641 ttcctgaatc cctggattac tggacctacc caggctcact gaccacccct cctcttctgg
     2701 aatgtgtgac ctggattgtg ctcaaggaac ccatcagcgt cagcagcgag caggtgttga
     2761 aattccgtaa acttaacttc aatggggagg gtgaacccga agaactgatg gtggacaact
     2821 ggcgcccagc tcagccactg aagaacaggc aaatcaaagc ttccttcaaa gactacaagg
     2881 acgacgacga caagtgagcg gccgcgcccc tctccctccc ccccccctaa cgttactggc
     2941 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     3001 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     3061 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     3121 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3181 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     3241 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     3301 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     3361 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     3421 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     3481 tatggccaca accatggcgt ccggaatgga gagcgacgag agcggcctgc ccgccatgga
     3541 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg
     3601 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct
     3661 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac
     3721 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa
     3781 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta
     3841 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag
     3901 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat
     3961 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta
     4021 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct
     4081 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga
     4141 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg
     4201 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg
     4261 ctagagctga atctaagtcg acggatctgc gatcgctccg gtgcccgtca gtgggcagag
     4321 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     4381 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     4441 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     4501 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     4561 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     4621 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     4681 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     4741 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     4801 agatccaagc tgtgaccggc gcctactcta gagccgccac catgtccaga ttagataaaa
     4861 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
     4921 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa
     4981 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
     5041 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta
     5101 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag
     5161 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
     5221 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg
     5281 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc
     5341 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct
     5401 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt
     5461 ccgcgtacag cggatcccgg gagttcagat cttatgaggg cagaggaagt cttctaacat
     5521 gcggtgacgt ggaggagaat cccggccctg aattcatgac cgagtacaag cccacggtgc
     5581 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     5641 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     5701 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     5761 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     5821 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     5881 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     5941 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6001 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6061 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6121 gcacctggtg catgacccgc aagcccggtg cctgagtcga caatcaacct ctggattaca
     6181 aaatttgtgg ttaactaact aaacttgttt attgcagctt ataatggtta caaataaagc
     6241 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6301 tccaaactca tcaatgtatc ttatcatgtc tggaattgac tcaaatgatg tcaattagtc
     6361 tatcagaagc tcatctggtc tcccttccgg gggacaagac atccctgttt aatatttaaa
     6421 cagcagtgtt cccaaactgg gttcttatat cccttgctct ggtcaaccag gttgcagggt
     6481 ttcctgtcct cacaggaacg aagtccctaa agaaacagtg gcagccaggt ttagccccgg
     6541 aattgactgg attccttttt tagggcccat tggtatggct ttttccccgt atccccccag
     6601 gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct
     6661 tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga
     6721 ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca
     6781 tccctggggg ctttgggggg gggctgtccc tgatatctat aacaagaaaa tatatatata
     6841 ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg agttaaatct
     6901 taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt tatagttcaa
     6961 aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga
     7021 gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa
     7081 gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaatctagc tgcatcagga
     7141 tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat
     7201 cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc
     7261 cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg
     7321 ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac
     7381 cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa
     7441 cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg
     7501 tgcggcgctg ggatattacg tcagcgagga cgggtatcct ggctggatgc cgcagaaatg
     7561 gacatggata ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg
     7621 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7681 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7741 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7801 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7861 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7921 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7981 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8041 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8101 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8161 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8221 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8281 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8341 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8401 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8461 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8521 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8581 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8641 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8701 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8821 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8881 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8941 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9001 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9061 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9361 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9601 ataagggcga cacggaaatg ttgaatactc at
//
