LOCUS       sequence-471678-        9975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System 
            Biosciences) expressing human SOD1gene including flag -tag 
            (DYKDDDDK) on the 3' end.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Rynes J, Istvankova E, Dzurov Krafcikova M, Luchinat E, Barbieri L, 
            Banci L, Kamarytova K, Loja T, Fafilek B, Rico-Llanos G, Krejci P, 
            Macurek L, Foldynova-Trantirkova S, Trantirek L
  TITLE     Protein structure and interactions elucidated with in-cell NMR for 
            different cell cycle phases and in 3D human tissue models.
  JOURNAL   Commun Biol. 2025 Feb 7;8(1):194. doi: 10.1038/s42003-025-07607-w.
  PUBMED    39920376
REFERENCE   2  (bases 1 to 9975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2025-02-7"; volume: "8(1):194. doi"; pages: " 
            10.1038/s42003-025-07607-w"
COMMENT     Sequence Label: pB-CuR-hSOD1
FEATURES             Location/Qualifiers
     source          1..9975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1421..1724
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             3076..3099
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(3112..3686,3687..3689,3690..3698)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3279..3296)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3506..3525
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3735..4397
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     promoter        4518..4729
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4742..5010
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5038..5047
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5050..5076
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5056..5082
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5194..5802
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     CDS             5839..5892
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5899..6498
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5899..5918)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6395..6415
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6545..6666
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6582..6601)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6636..6655
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7429..7463
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7954..7970)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7954..7970)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7967..7989)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7978..7994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8002..8008,8009..8026,8027..8032))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8047..8068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8185..8202)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8356..8944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8436..8455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9115..9906,9907..9975))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9738..9757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagttattaa tagtaatcaa
     1381 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1441 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1501 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1561 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
     1621 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     1681 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     1741 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     1801 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1861 atacccccgc cccgttgacg caaatgggca agcttgccgg gtcgaggtag gcgtgtacgg
     1921 tgggaggcct atataagcaa ccggtataat caaacagacc agattgtctg tttgttaccg
     1981 gtgtttagtg aaccgggcgc gcctcatatc gcctggagac gccatccacg ctgttttgac
     2041 ctccatagaa gacaccggga ccgatccagc ctccgcggtc actctcttcc gcatcgctgt
     2101 ctgcgagggc cagctgttgg gctcgcggtt gaggacaaac tcttcgcggt ctttccagta
     2161 ctcttggatc ggaaacccgt cggcctccga acggtactcc gccaccgagg gacctgagcg
     2221 agtccgcatc gaccggatcg gaaaacctct cgagaaaggc gtctaaccag tcacagtcgc
     2281 aaggtaggct gagcaccgtg gcgggcggca gcgggtggcg gtcggggttg tttctggcgg
     2341 aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag acggcggatg gtcgaggtga
     2401 ggtgtggcag gcttgagatc cagctgttgg ggtgagtact ccctctcaaa agcgggcatt
     2461 acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc
     2521 gatctggcca tacacttgag tgacaatgac atccactttg cctttctctc cacaggtgtc
     2581 cactcccagg tccaagtttg gtctagagct agcatggcga cgaaggccgt gtgcgtgctg
     2641 aagggcgacg gcccagtgca gggcatcatc aatttcgagc agaaggaaag taatggacca
     2701 gtgaaggtgt ggggaagcat taaaggactg actgaaggcc tgcatggatt ccatgttcat
     2761 gagtttggag ataatacagc aggctgtacc agtgcaggtc ctcactttaa tcctctatcc
     2821 agaaaacacg gtgggccaaa ggatgaagag aggcatgttg gagacttggg caatgtgact
     2881 gctgacaaag atggtgtggc cgatgtgtct attgaagatt ctgtgatctc actctcagga
     2941 gaccattgca tcattggccg cacactggtg gtccatgaaa aagcagatga cttgggcaaa
     3001 ggtggaaatg aagaaagtac aaagacagga aacgctggaa gtcgtttggc ttgtggtgta
     3061 attgggatcg cccaagacta caaggacgac gacgacaagt gagcggccgc gcccctctcc
     3121 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     3181 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     3241 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     3301 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     3361 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     3421 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     3481 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     3541 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     3601 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     3661 tttcctttga aaaacacgat gataatatgg ccacaaccat ggcgtccgga atggagagcg
     3721 acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg
     3781 tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca
     3841 agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg
     3901 gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg
     3961 ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc
     4021 tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg
     4081 tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg
     4141 ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca
     4201 ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca
     4261 agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg
     4321 tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga
     4381 cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg
     4441 ccggacccgg ctccaccgga tctcgctaga gctgaatcta agtcgacgga tctgcgatcg
     4501 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     4561 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     4621 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     4681 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
     4741 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
     4801 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
     4861 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
     4921 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
     4981 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ctctagagcc
     5041 gccatggctt acccatacga tgttccagat tacgctagtt tggtgaggca gcagagaccg
     5101 atgacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa gtttggaaga
     5161 tctaccatgc caaagagacc cagacccgtg atcatgagtc caaagagaag aacacaggca
     5221 gagcgcgcaa tggagaccca gggcaagttg attgcagcgg ccctgggggt tttacgggaa
     5281 aaaggttacg cgggattccg gatcgcagat gtgcccggtg ctgcaggtgt ctcgagagga
     5341 gcgcagagcc atcatttccc gacaaagctt gagcttctgc ttgccacttt tgaatggctt
     5401 tacgaacaga tcaccgaacg cagtcgggct cgattagcga aattgaagcc agaggatgac
     5461 gtcatccagc aaatgctgga cgacgccgcc gaatttttcc tcgacgatga cttctctatc
     5521 agccttgatt tgattgtggc tgccgaccgg gatccagcgt tacgcgaggg tattcagcgc
     5581 acggtagaga ggaatcggtt tgtcgtcgag gatatgtggc ttggtgttct ggtgagccgt
     5641 ggtctttcgc gtgatgatgc agaagatatc ctttggttga tattcaattc ggtgcgtggg
     5701 cttgctgttc gtagcctatg gcagaaggac aaagaacgct ttgagcgtgt caggaactcg
     5761 acactcgaaa ttgcgcgaga gcggtacgcg aaattcaagc gcgcggccaa accctctggc
     5821 cggtctctct ctgttcgaga gggcagagga agtcttctaa catgcggtga cgtggaggag
     5881 aatcccggcc ctgaattcat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5941 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6001 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6061 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6121 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6181 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6241 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6301 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6361 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6421 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6481 cgcaagcccg gtgcctgagt cgacaatcaa cctctggatt acaaaatttg tggttaacta
     6541 actaaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6601 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6661 atcttatcat gtctggaatt gactcaaatg atgtcaatta gtctatcaga agctcatctg
     6721 gtctcccttc cgggggacaa gacatccctg tttaatattt aaacagcagt gttcccaaac
     6781 tgggttctta tatcccttgc tctggtcaac caggttgcag ggtttcctgt cctcacagga
     6841 acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt
     6901 ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa
     6961 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg
     7021 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg
     7081 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     7141 ggggggctgt ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag
     7201 tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag
     7261 ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
     7321 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
     7381 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
     7441 ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt
     7501 ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc
     7561 gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct
     7621 gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc
     7681 acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt
     7741 tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg
     7801 acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt
     7861 acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg
     7921 agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7981 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8041 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8101 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8161 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8221 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8281 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8341 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8401 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8461 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8521 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8581 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8641 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8701 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8761 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8821 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8881 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8941 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9001 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9061 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9121 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9181 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9241 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9301 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9361 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9421 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9481 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9541 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9601 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9661 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9721 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9781 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9841 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9901 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9961 atgttgaata ctcat
//
