LOCUS       sequence-471686-       16435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express flourescent protein AmCyan from the Foxtail Mosaic Virus 
            Vector (T-DNA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16435)
  AUTHORS   Baysal C, Kausch AP, Cody JP, Altpeter F, Voytas DF
  TITLE     Rapid and efficient in planta genome editing in sorghum using 
            foxtail mosaic virus-mediated sgRNA delivery.
  JOURNAL   Plant J. 2025 Jan;121(2):e17196. doi: 10.1111/tpj.17196. Epub 2024 
            Dec 11.
  PUBMED    39661735
REFERENCE   2  (bases 1 to 16435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2025-01"; volume: "121(2):e17196. doi"; pages: " 
            10.1111/tpj.17196. Epub 2024 Dec 11"
COMMENT     Sequence Label: pJPC596 FoMV-AmCyan
FEATURES             Location/Qualifiers
     source          1..16435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(25..822)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(136..155)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     746..765
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(885..1561)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    1752..1773
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1788..1793,1794..1811,1812..1818)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1826..1842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1831..1853
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1850..1866
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1850..1866
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2231..2576
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      3173..3182
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7907..8596
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan1
                     /note="mammalian codon-optimized"
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPLAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKMTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHAVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPF"
     terminator      9577..9829
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9847..9871
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11172..11801
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12230..13303
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13369..13563
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13799..13821)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13907..14047
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13962..13981
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14062..14079)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14233..14821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14313..14332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14908..15702)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    16127..16151
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    16229..16403
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 tcgatccgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
       61 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
      121 gccaagttct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
      181 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
      241 caagcaggca tcgccatgtg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
      301 cctggcgaac agttcggctg gcgcgagccc ctgatgttct tcgtccagat catcctgatc
      361 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
      421 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
      481 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
      541 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
      601 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgg agttcattca gggcaccgga
      661 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgaa acacggcggc
      721 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
      781 ggccggagaa cctgcgtgca atccatcttg ttcaatcccc atggtcgatc gacagatctg
      841 cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg tgtcctctcc
      901 aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg
      961 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
     1021 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
     1081 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
     1141 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
     1201 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
     1261 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc
     1321 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
     1381 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
     1441 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
     1501 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
     1561 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
     1621 gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt
     1681 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     1741 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     1801 cttccggctc gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     1861 tatgacatga ttacgaattc gagctcctcc aaaaatatca aagatacagt ctcagaagac
     1921 caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat
     1981 tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa
     2041 tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc
     2101 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
     2161 tcaaagcaag tggattgatg tgatactcca aaaatatcaa agatacagtc tcagaagacc
     2221 aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt
     2281 gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat
     2341 gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca
     2401 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     2461 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     2521 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag agggaaaact
     2581 cttccgaaac cgaaactgac tgaaactacc tcgaccgacc ttagaaccca agaacccaac
     2641 gggtgcggcc actatgtcca tcgaggcagt tttcgaccag gttaccgacc catcgctccg
     2701 cgctgtgatt caggaggaag cgcacaaaca gatcaaagat ttatttaagg aaacgacgcg
     2761 ctgcaatccc tactccatac cgcaagctgg gcgcaaggtt ttggaaaagt acgccatccc
     2821 ctacaacccg tactctctca aactacaccc tcacgcagcc tcaaaagcgt ttgaagtgtc
     2881 gctctacgag gctgcgtcta actacctccc ctccacctcc tcaactcctg tcacattcat
     2941 gttcacaaaa ccgggcaagc tcagattctt taggcgccga ggtcatgtgg acaaattcgt
     3001 taatgctgac atagttccaa gagacttggc tagataccca cgcgacacag tctacagtta
     3061 tctgcccgag atcaccacca cacacgcttt cattggcgac accctgcatc acttcggtga
     3121 ggactttctc gtcgaggttt tctccaggtc accgaaacta gaagtgcttc tagccaccat
     3181 ggtattacca cccgaagcct tttacagaat ggagtccctc catccctcgg tttacactct
     3241 cctctacagg gacgaccgat tcctatacct gcctggtggc ctatctggcg gtgagtacga
     3301 acatcgctat aaggacctaa actggctaac atttggcacg gttacgcacg gcgggatcac
     3361 tatcacagga gaacgcattg agactaaggc cgcgaatcat cttttcctct tcagacgagg
     3421 gcgactagcg acaccaaaat tccgctcatt cgatatgccc gagcctatgg tcctgcttcc
     3481 caaggttttc cgccccgcaa agtacaatgt acagaagcca attccccggg agaaagcaaa
     3541 caaatggttg atgtacgtta aatccatcgg caatgccacc attcgtgacg tatgggctaa
     3601 gctgaggcaa accatagcca atgcagacat tggactcttc tcgcccactg agctcgtgca
     3661 tctcacgaat tacttcctgc tcctgggccg gcttgactca cacaactcct tcgaccaagt
     3721 actggccgac agtgtgctga aagcatggtt cagaccaatg gtcgcaaagc ttcaggagat
     3781 caagcacaaa ctcatggggc agacccaatt catgcaactc tgccaagcgc tagagatgac
     3841 ggaggtggac ctcgtctttg aggtccggga ctccaagacc ccccacaaac aagctgtgcc
     3901 attggaccgt gaaattgaaa acgttttgtt ggaaggagtc tcatcggagc caacttacac
     3961 tgaaaccgaa ggcgttgctg atggtccact tcccccccca atgcaaactg cagccgagcc
     4021 gtccgcgacc tcagacgagc ctgagagctc tagctcgcgt gaaattgagc accaaccggc
     4081 gcctgagatc acgcttgacg aggaagaacc tcagcgagac gatctgcctt gggacgcttg
     4141 gagaacacaa ttaagggcgc ttggcttcga ggcctcagaa aggcagtatg acccggacgg
     4201 tgaactgatc tctcctatcc tgagcacccg aaggttgcct aaaactccca taaacacaac
     4261 actctgcgcc acgctagaca agattgcacg ctgcccaact ttctacaagc ctgacacaga
     4321 tcgcgcgcag acttacgctc gcgacgtcat ggcggggaaa accggtgcta ttctcaagca
     4381 acaacccttt gagtggaaaa ccacgctcaa gcgcaagact aaagaggaac cgaaggaaat
     4441 tcaccttgcg gtgttgcatg gtgcgggcgg gtcgggcaaa tcctacgcac tgcaggaatt
     4501 tatgcggaac aaccctgaca caccaattac ggtcatcctg ccgactaacg agctcagggc
     4561 cgactggaag aaaaaattgc ccgcccacga caaagacaca tttatgacat acgaaaacgc
     4621 gctcttgtgc cctcgtggag acatcttcat tatggacgat tacacaaaat tgcccagggg
     4681 ctacattgag gctttcgtgc agaatgcacc tgccctctca cttctgatac tcaccggtga
     4741 ccccaaccaa gccgaacact ttgagaccac tgaggacaat gaaattaaca gcctcgcccc
     4801 cgcctcagtg gtcttcggca agttctctag gtaccacata aatgccacac accgcaaccc
     4861 cagaaacttg gcaaacgccc tcggtgttta ctccgagacg cccggggagg ttaaagtgct
     4921 ttacacgagg aacatcaaga ccggttatca caatctcgtg ccctcacaaa tgaagatgag
     4981 aaattacgcc tcactcgggc agcgagcgtc cacctatgcg ggttgtcagg ggatcaccgc
     5041 gccccgcgtt caaatcatcc tagactccga cacaccccgg tgcaccaggc aagtcatgta
     5101 cactgcactc tcaagggcca cgacggaagt ggtgctctgc aacacgatgc cggatgagaa
     5161 aagctttttc cagaaggttg aagcaacacc gtacctcaaa gccatcctca acctcaacaa
     5221 agagattaaa gtcactgagg gtgacttgac agaagaaccg ccgagggagc ccgctcctcc
     5281 caccacacac ctacctgttg aaaacagaat tattcttaat gaggccctag tcgaaccgct
     5341 gcccgacaaa catgaccgcg agatctactc caactccact ggcttttcaa actgcataca
     5401 gactcaagac ccgtacatcc aagccttcca acatcagcag gccaaggacg agacattgtt
     5461 ctgggcaacc gtcgagaaga ggctcgcagc atctacgccg aaggacaact ggacagaatt
     5521 caagaccaag agacctctgg gtgacgtgct ttggctcgcg tacaagcggg cgatggagct
     5581 cccagatgag cccatcaaat ttaacccaga gctctggtgg gcatgtgcag acgaggtgca
     5641 aaagacctac ctctccaagc ccatacacgc actcaagaac ggaatccttc ggcaatcacc
     5701 cgactttgac tggaacaaac tgcagatttt cctcaagtca cagtgggtta agaagattga
     5761 caaaatcggg aaaattgacg tcaacgctgg acagacaatt gccgcctttt accaaccaac
     5821 cgttatgctg tttggaacca tggcgagata catgcgccgc atccgcgaca cttatcaacc
     5881 ccgcgaaata ctcatcaatt gcgagaagaa ccagaagcac atttcgaagt gggtcgagag
     5941 caattggaac caccgcctac ctgcttacac caatgacttc accgcttacg accaaagcca
     6001 agacggggct atgttacagt ttgaggtgct gaaagccctg caccatgata tccctcatga
     6061 ggttgtggaa gcctacgtag cccttaaact caactcaaaa atgtttctgg gcacactggc
     6121 gattatgaga ttaactggtg agggacccac cttcgacgct aacacagagt gcaacattgc
     6181 ttacacacac gcccggttcg agatcccaaa gaatgtggcg caaatgtacg cgggtgacga
     6241 ctgtgcgctc aactgcaggc ccgttgaacg gcagtccttc ctgcctcttg tggagaaatt
     6301 caccctgaaa tcaaaaccta aagtatttga gcaaaaagtt gggtcatggc ctgagttctg
     6361 cggcaatctg atcaccccac ggggctacct caaggatccc atgaagctac aacactgcct
     6421 gcaactggca cagaggaaga aaccatccga acctgggtcg cttaaagacg ttgctgagaa
     6481 ctacgctatg gacttactac ccacatacga gctaggggat gcactctacg aaatcttcga
     6541 cgagagacaa atgaacgcgc actatcagtc ggtcaggacg cttatcacat gcgcccacac
     6601 caaagtcctc cgagttgcac aggcacttca ggaagactgc accttcttta gctccatcta
     6661 acaggttttg agttaggcta actccactga cgaattgaat aacaatggat agtgaaatag
     6721 ttgaacgact aacaaagctt ggtttcgtca agacttcaca cacgcacatc gctggcgagc
     6781 ccctcgtgat tcacgccgtt gctggggccg gtaaaaccac cctccttcgg tccttacttg
     6841 aattacctgg agtggaagtc ttcacaggcg gggagcacga tcctccaaat ttgtcaggga
     6901 aatatatccg ctgcgctgca ccccctgtgg ccggtgcata caacattctt gacgagtacc
     6961 ccgcgtaccc aaattggcga tcgcaaccct ggaacgtcct aatcgccgac aacctacaat
     7021 acaaagaacc cacagctcgc gcccactaca catgcaatcg cactcaccgc ctggggcagc
     7081 tcactgtcga cgctttgcgt agggttggtt tcgacatcac ctttgccggc acgcagactg
     7141 aagactacgg attccaggaa ggccatctct acactagtca attctacgga caggtcattt
     7201 cacttgacac gcaggcccat aagatcgctg tgcgccacgg actcgcaccc ctgtccgctt
     7261 tagaaacccg ggggctggaa tttgatgaga ccactgtgat aacgactaaa acctcgctgg
     7321 aggaagtgaa ggacaggcac atggtctatg tcgctctcac aaggcacagg cgcacctgcc
     7381 atctctacac cgctcacttt gcgccctccg cctgacaaca cgaaagccat actaactgta
     7441 gctataggaa tagccgcctc cctagtcttt ttcatgctca cacgcaacaa tctgccacac
     7501 gtcggtgata acatccactc actaccccac ggaggaagtt atattgacgg taccaagtcc
     7561 atcaactacc gcccacctgc gtcacgctac ccctcatcca acttactcgc tttcgctcca
     7621 ccaatactcg ccgcagtact ctttttcctc acacagccat atctagctac cagacgatcc
     7681 aggtgcgttc ggtgcttcgt tgtccacggg gcatgcacga atcacaccta gttgttatat
     7741 tagcgctgtt acttatagct ctgtggtgtc taagcactcg acccgttcaa ccatcgtgcc
     7801 acgtcgaaat caacggccac tccatcatcg tcaccggaaa ctgctggcac tccacacagc
     7861 gtccccactg agggtgttag ggtaaccaac atcagtgaag agaaacatgg ccctgtccaa
     7921 caagttcatc ggcgacgaca tgaagatgac ctaccacatg gacggctgcg tgaacggcca
     7981 ctacttcacc gtgaagggcg agggcagcgg caagccctac gagggcaccc agacctccac
     8041 cttcaaggtg accatggcca acggcggccc cctggccttc tccttcgaca tcctgtccac
     8101 cgtgttcatg tacggcaacc gctgcttcac cgcctacccc accagcatgc ccgactactt
     8161 caagcaggcc ttccccgacg gcatgtccta cgagagaacc ttcacctacg aggacggcgg
     8221 cgtggccacc gccagctggg agatcagcct gaagggcaac tgcttcgagc acaagtccac
     8281 cttccacggc gtgaacttcc ccgccgacgg ccccgtgatg gccaagatga ccaccggctg
     8341 ggacccctcc ttcgagaaga tgaccgtgtg cgacggcatc ttgaagggcg acgtgaccgc
     8401 cttcctgatg ctgcagggcg gcggcaacta cagatgccag ttccacacct cctacaagac
     8461 caagaagccc gtgaccatgc cccccaacca cgcggtggag caccgcatcg ccagaaccga
     8521 cctggacaag ggcggcaaca gcgtgcagct caccgagcac gccgtggccc acatcacctc
     8581 cgtggtgccc ttctaggccc cactcaacga ccgcattgag ggtgttaggg taaccaacat
     8641 cagtgaagag aaacaaccca cctcaagtgt gacctcatca tttcaggaca caatggcaac
     8701 acaaaatgcc gacgtcactg atgcgacgga ctacaagaaa ccgcctgctg aaactgagca
     8761 gaaggcactc accattcaac cacggtcaaa caaggcgccc agtgacgagg agttggtacg
     8821 catcatcaac gcggcgcaga agcgaggcct cacacccgcg gcctttgttc aagcagctat
     8881 agtcttcacc atggaatcca tggacaaggg cgccaccgac tccacgattt tcacgggaaa
     8941 atacaacact ttcccaatga aaagtctggc gctagcttgc aaagatgctg gcgtgcccgt
     9001 gcacaaactt tgctacttct ataccaagcc ggcttacgcg aaccgtaggg tcgccaacca
     9061 gccgcctgct cgctggacta acgagaatgt gcccaaagct aacaagtggg cggctttcga
     9121 caccttcgac gcacttctcg acccatacgt agtcccatcc tctgtaccgt acgatgagcc
     9181 cacgccagag gatcgccaag tcaatgagat tttcaagaag gacaatttga gtcaggcagc
     9241 atccagaaac caactcctag gaacgcaagc ctccatcacg cgcgggagac tcaacggcgc
     9301 accagcacta ccaaacaacg ggcagtactt catcgaggca cctcagtaat ctagaagatg
     9361 ggactcgagc gatcagtagt atgataccaa taaataaatc gggcgaatcc gcgcctcctg
     9421 actatgggca ggtttacgga ccaagctgta tcgagatacg acctaacagt aacgcagcta
     9481 aggggtgaat gcacacatcg cttataaaaa aaaaaaaaaa aaaaaaaaac ctggatccta
     9541 ggttcacaaa gtgtcatcga tagctcgaat ttccccgatc gttcaaacat ttggcaataa
     9601 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     9661 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     9721 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     9781 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaaactatc
     9841 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     9901 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     9961 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc
    10021 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
    10081 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
    10141 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
    10201 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
    10261 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
    10321 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
    10381 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
    10441 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
    10501 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
    10561 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
    10621 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
    10681 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
    10741 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
    10801 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
    10861 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
    10921 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cggctcaacc
    10981 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
    11041 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
    11101 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
    11161 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
    11221 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
    11281 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
    11341 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
    11401 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
    11461 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
    11521 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
    11581 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
    11641 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
    11701 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
    11761 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
    11821 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
    11881 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
    11941 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
    12001 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
    12061 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
    12121 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
    12181 cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
    12241 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
    12301 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
    12361 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
    12421 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
    12481 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
    12541 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
    12601 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
    12661 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
    12721 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
    12781 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
    12841 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
    12901 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
    12961 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
    13021 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
    13081 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
    13141 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
    13201 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
    13261 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
    13321 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac
    13381 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
    13441 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
    13501 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
    13561 ataactgtct ggccagcgca cagcccaaga gctgcaaaaa gcgcctaccc ttcggtcgct
    13621 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
    13681 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
    13741 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
    13801 tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca
    13861 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
    13921 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
    13981 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    14041 catcaggccc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    14101 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    14161 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    14221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    14281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    14341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    14401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    14461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    14521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    14581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    14641 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    14701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    14761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    14821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    14881 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
    14941 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
    15001 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
    15061 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    15121 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    15181 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    15241 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    15301 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    15361 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    15421 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    15481 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    15541 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    15601 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    15661 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    15721 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    15781 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    15841 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
    15901 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
    15961 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
    16021 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
    16081 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    16141 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    16201 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
    16261 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
    16321 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
    16381 actcacacat tattatggag aaactcgagc ttgtcgatcg actctagcta gagga
//
