LOCUS       sequence-471733-        8807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For overexpression of mEGFP-NUP98-DDX10-Mut1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Zhang Y, Stoppelkamp I, Fernandez-Pernas P, Allram M, Charman M, 
            Magalhaes AP, Piedavent-Salomon M, Sommer G, Sung YC, Meyer K, Grams
            N, Halko E, Dongre S, Meierhofer D, Malszycki M, Ilik IA, Aktas T, 
            Kraushar ML, Vastenhouw N, Weitzman MD, Grebien F, Niskanen H, Hnisz
            D
  TITLE     Probing condensate microenvironments with a micropeptide killswitch.
  JOURNAL   Nature. 2025 Jun 4. doi: 10.1038/s41586-025-09141-5.
  PUBMED    40468084
REFERENCE   2  (bases 1 to 8807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Jun 4. doi: 10.1038/s41586-025-09141-5."
COMMENT     Sequence Label: pRK5_mEGFP-NUP98-DDX10-Mut1
FEATURES             Location/Qualifiers
     source          1..8807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      934..943
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(940..942,943..945,946..1656)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(985..1006)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1246..1265)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1593..1614
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(4802..4828)
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     regulatory      5107..5116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5120..5254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5157..5176)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5211..5230
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        5323..5680
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5323..5514
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5346..5366)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5531..5666
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5700..5717)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5700..5716)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5709..5731)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5929..6384
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6016..6035)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6226..6247
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6401..6420)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6520..6542
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6580..6598)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6666..6770
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6771..6839,6840..7631)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6989..7008)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7802..8390
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8291..8310
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8544..8561
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8678..8699
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8714..8719,8720..8737,8738..8744)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8752..8768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8757..8779
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8776..8792
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8776..8792
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcgtaccg gtcgccacca tggtgagcaa gggcgaggag
      961 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1021 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1081 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1141 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1201 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1261 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1321 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1381 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1441 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1501 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     1561 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1621 gccgggatca ctctcggcat ggacgagctg tacaagaccg gcggcatgtt taacaaatca
     1681 tttggaacac cctttggggg tggcacaggt ggctttggca caacttcaac atttggacag
     1741 aatactggct ttggcactac tagtggaggg gcatttggaa catctgcatt tggttctagc
     1801 aacaatactg gaggcctctt tggaaattca cagactaaac caggaggatt gtttggaacc
     1861 agttcattta gccagccagc tacctccaca agcactggct ttgggtttgg tacgtcaaca
     1921 ggaacagcaa ataccttgtt tggaactgca agcacagggg gcctctttgg aaccagtctc
     1981 ttctcatccc aaaacaatgg actctttgga gcctttgcac aaaataaacc aactggcttt
     2041 ggcaattttg gaaccagtac tagcagtgga ggactctttg gaaccacaaa taccacctct
     2101 aatccttttg gcagcacatc tggctccctc tttgggccaa gtagttttac agctgctcct
     2161 actgggacta ctattaaatt taaccctcca actggtacag atactatggt caaagctgga
     2221 gttagcacta acataagtac caagcaccag tgtattactg ctatgaaaga atatgaaagc
     2281 aagtcactag aggaacttcg tttagaggat tatcaggcta acaggaaggg cccacagaac
     2341 caggtgggag caggtaccac aactggcttg tttgggtctt ctccagccac ttccagcgga
     2401 ttgtttggag caacaggact cttcagctcc tccaccacta attcaggctt gtttgggggc
     2461 tttgcatatg gtcagaacaa aactgccttt ggaactagta caactggatt tggaacaaat
     2521 ccaggtggtc tctttggcca acagaatcag cagactacca gcctcttcag caaaccattt
     2581 ggccaggcta caaccaccca gaacactggc ttttcctttg gtaataccag caccatagga
     2641 cagccaagca ccaacaccat gggattattt ggagtaaccc aagcctcaca gcctggaggt
     2701 ctttttggga cagctacaaa caccagcact gggacagcat ttggaacagg aacaggtctc
     2761 tttgggcaga ccaatactgg atttggtgct gttggttcga ccctgtttgg caataacaag
     2821 cttactacat ttggaagcag cacaaccagt gcaccttcat ttggtacaac cagtggcggg
     2881 ctctttggtt ttggcacaaa taccagtggg aatagtattt ttggaagtaa accagcacct
     2941 gggactcttg gaactgggct tggtgcagga tttggaacag ctcttggtgc tggacaggca
     3001 tctttgtttg ggaacaacca acctaagatt ggagggcctc ttggtacagg agcctttggg
     3061 gcccctggat ttaatactac gacagccact ttgggctttg gagcccccca ggccccagta
     3121 gttcttgatg aagcagatag aatcttggat atgggctttg ctgataccat gaatgctgtt
     3181 attgaaaatc tccccaagaa acgtcagact ttacttttct cagcaacaca aactaaatct
     3241 gtaaaggacc ttgcacgctt gagtttgaaa aaccctgagt atgtctgggt tcatgaaaaa
     3301 gcaaaatata gcacccctgc cactttggaa cagaactaca tagtctgtga gctgcagcaa
     3361 aaaataagtg tgctgtattc ctttttgaga agccatctga agaagaagag cattgtattt
     3421 ttttccagtt gcaaagaggt ccagtatctg taccgagtgt tttgccggct acgtcctggt
     3481 gtttctatcc ttgcactcca tggtcgacag cagcaaatga gaagaatgga agtctataat
     3541 gagtttgtcc gtaagagagc tgcagtactc tttgctactg atattgcagc caggggtctg
     3601 gatttcccgg ccgtgaattg ggttcttcag tttgattgtc ctgaggatgc caacacatat
     3661 attcacagag caggtagaac tgccaggtac aaagaggatg gtgaagcttt gctaattttg
     3721 ctaccctcag aaaaagctat ggtgcagcag cttcttcaga agaaagtacc tgtgaaggaa
     3781 atcaaaatca atccagaaaa acttatagat gtccagaaaa aattggaatc tattttagct
     3841 caagatcaag atttaaaaga aagagctcaa aggtgtttcg tctcctatgt acgatctgta
     3901 tatctgatga aggataaaga agtatttgat gtgagcaagt tacctatacc tgaatatgcc
     3961 ctgtctcttg gtcttgctgt ggcaccacgc gtaagatttc ttcagaaaat gcagaaacaa
     4021 cccaccaaag aattggtaag gagccaagcc gataaagtaa ttgagccaag ggctccctcc
     4081 ctcaccaatg acgaagtgga agaatttaga gcctacttca atgagaaaat gtccatcctt
     4141 caaaaaggtg gaaaaagact cgaagggaca gagcacagac aggataatga tactggtaat
     4201 gaagaacagg aagaagaaga agacgatgaa gaagaaatgg aagagaaact ggcaaaagca
     4261 aaaggatctc aagccccatc tcttcctaac accagtgagg cacagaagat caaggaagtt
     4321 cctacacagt tcttggacag agatgaggag gaagaagatg ctgatttctt gaaggtgaag
     4381 cggcataatg tgtttggatt ggaccttaaa gacgagaaaa cattacagaa gaaagaacct
     4441 tctaaatcca gcatcaagaa aaaaatgacc aaagttgcag aagcaaaaaa agtaatgaag
     4501 agaaatttta aagtgaataa gaagataaca tttactgatg aaggggagtt ggttcagcag
     4561 tggccacaaa tgcagaaatc tgccatcaag gatgctgagg aagatgatga cacaggtggt
     4621 atcaacttac ataaagcaaa ggaaagactt caggaagagg acaaatttga caaagaagaa
     4681 tataggaaaa aaattaaggc aaagcatcgg gagaaaagac tgaaagaaag ggaagccaga
     4741 agagaagcca acaagagaca agcaaaggcc aaagatgaag aggaagcctt tctggattgg
     4801 agtgatgatg atgatgatga tgatgatgga tttgatccaa gcacactccc agatccagat
     4861 aaatacagaa gctctgaaga ttcagatagt gaagatatgg aaaataaaat aagtgatacc
     4921 aagaagaagc aggggatgaa gaagaggagc aacagtgaag tggaagacgt gggaccaaca
     4981 agtcataaca gaaagaaggc caggtgggac actttagagc ctttggatac cggcctgtct
     5041 ttagcagagg atgaagagct ggtgttacat ctgctaagaa gtcaaagcta aacctgcaga
     5101 agcttggccg ccatggccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     5161 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5221 caaactcatc aatgtatctt atcatgtctg gatcgggaat taattcggcg cagcaccatg
     5281 gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg gaaagaacca
     5341 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     5401 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     5461 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5521 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5581 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5641 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctgttaa cagcttggca
     5701 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5761 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5821 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     5881 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     5941 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6001 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6061 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6121 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     6181 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6241 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
     6301 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     6361 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     6421 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     6481 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     6541 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     6601 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     6661 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6721 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6781 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6841 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6901 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6961 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7021 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7081 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7141 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7201 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7261 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7321 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7381 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7441 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7501 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7561 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7621 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7681 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7741 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7801 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7861 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7921 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     7981 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8041 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8101 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8161 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8221 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8281 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8341 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8401 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8461 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8521 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     8581 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     8641 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     8701 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     8761 gataacaatt tcacacagga aacagctatg acatgattac gaattaa
//
