LOCUS       sequence-471744-        6424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses untagged TaTasR from a PBAD promoter, followed by the 
            native TIGR array (8 units).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Faure G, Saito M, Wilkinson ME, Quinones-Olvera N, Xu P, 
            Flam-Shepherd D, Kim S, Reddy N, Zhu S, Evgeniou L, Koonin EV, 
            Macrae RK, Zhang F
  TITLE     TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in 
            prokaryotes and their viruses.
  JOURNAL   Science. 2025 Feb 27:eadv9789. doi: 10.1126/science.adv9789.
  PUBMED    40014690
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2025 Feb 27:eadv9789. doi: 10.1126/science.adv9789."
COMMENT     Sequence Label: pMW357
FEATURES             Location/Qualifiers
     source          1..6424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(53..931)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPPRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        958..1242
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1165..1184
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             1269..1291
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(3164..3181)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3334..3420
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3512..3539
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3558..3649
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3650..3718,3719..4510)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGEPLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3868..3887)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4552..5007
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4639..4658)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4849..4870
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      5118..5706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5607..5626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5860..5877
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5892..6032
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5958..5977)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6118..6140
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6278..6297
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca
       61 acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg
      121 ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc
      181 gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac
      241 gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca
      301 gacgcggcgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg
      361 ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga
      421 gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca
      481 gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc
      541 tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg
      601 gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc
      661 attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa
      721 tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa
      781 tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat
      841 aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg
      901 gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag
      961 aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct
     1021 cgctaaccaa accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag
     1081 ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt
     1141 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggata
     1201 ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt ttttgggcta
     1261 gaaataattt tgtttaactt taagaaggag atatacatat ggataacgat atcacaatat
     1321 tggcggtcga ttggtctcat gaagaacgta aacttgcaat ttttgatggc aagaagataa
     1381 ggaaaaaact tcctgagcct tctagcgatg taataattgt ggctgaaaat attcctcaaa
     1441 aatatgccgc tccgttcatc gaggtagggg caaaagttct tagatgttcg acaaacgcca
     1501 cagcagatgc aagaaaaaac tatcaaaaga aagtcgatgc tgcttttgcg aaaaacgacg
     1561 aaaatgattc taaagtgatt tgggcgttat atcaaacaca cccagaattg tttcgtgaaa
     1621 tgaagcttga gccacccctt tcttcttatt atgctatttt taaagattac caagaggtaa
     1681 gaattagaac tggcaatcga ctctattcag atcgcactga tgcaatggaa gagtttttta
     1741 aaatagttaa gaaaggtgaa catgaactaa aaaaggctgt cgataaagaa ttagaaaatc
     1801 atccggtcta tacgcagtgg ttacagcata ttaaaggtat tggcccggtc gttgccgggg
     1861 gattgatatc gttaattgga gatatagatc gcttcgacag cgtttctaaa ttatgggcct
     1921 atgcaggtta tagtgttgat aatggaaaag tgcaaaagcg taaaaaaggt gtagcttcaa
     1981 attggaaaaa taaaattcgc acacattgtt acaacattgt agattcattt atcaaacaac
     2041 gcacttcagt ttatcgtgag ttgtatgatg ccgaaaaagc tcgtcaacgt ccaaaggtag
     2101 aatccgatgg tcatgctcac aaccgtgctg taagaaaagt tgctaaggta tttttacaac
     2161 attattgggt agtttctaga gaattagcag ggttttctgt gtctaaacct tggattttag
     2221 aacatggtgg gcacgttgat tacatcaaac caccacattg gaataaagta gaaattaagc
     2281 catgcaagcc ctgaaaccca ttgccttagt gcgagccatc atatatatgg aacccatgcc
     2341 gcagatacga gccaagcgaa aaatgaaacc caacgtcgct ttgcgagcca aactacatat
     2401 gaaacccaca atggagatgc gagccaaact acatatgaaa cccatgctgc tagatgcgag
     2461 ctaagcggaa aatgaaaccc aacaccgatt tgcgagccaa acgatctatg aaacccaagg
     2521 tctagttgcg agccagaagc cctttgaaac ccatcatcaa cgtgcgaaaa agcccgtttt
     2581 tcaacgggct tttatctttt cataacaagt tctcttactc tatcgtaaac accacaatat
     2641 tgacatttaa gtattaaatt agtgtctgtg gcattagaat acttatcttg agtaaaacta
     2701 atgactttat acacatgatg cccagataga caacaatata aatattttat aaggctcata
     2761 acatatttat gaaaaacggg cttttttatt atacataaca ctatgttata cttcaaaaag
     2821 tggttgttag aatttatggg gtctacagga gttgtgtttg atcccaaaca aaaatcaaga
     2881 gactggaatt ggctcggtgc ccccggctcc actggtgtct cccctaaaga agacccaatt
     2941 ggttgtaaaa agaaaaagaa gaaaaagaag aaatgagttg gtttaacgca ctatctaacc
     3001 cttttcaaca aagtgttaaa aaatttatct acgaacttat cggaccagaa cgatatatca
     3061 accatgatcc cattgtaggt ctgctggcta aaaatgttag cactgccgaa gagtatgaga
     3121 attcgaagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat
     3181 cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc
     3241 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
     3301 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa
     3361 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     3421 ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc
     3481 ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt
     3541 gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3601 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3661 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3721 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3781 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3841 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     3901 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3961 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4021 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4081 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4141 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     4201 ggcaacaacg ttgcgcaaac tattaactgg cgaaccactt actctagctt cccggcaaca
     4261 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4321 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4381 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4441 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4501 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt tacgcgccct
     4561 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4621 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4681 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4741 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     4801 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4861 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     4921 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4981 ttaacaaaat attaacgttt acaatttaaa aggatctagg tgaagatcct ttttgataat
     5041 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5101 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5161 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5221 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5281 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5341 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5401 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5461 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5521 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5581 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5641 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5701 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5761 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5821 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5881 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     5941 atctggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     6001 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     6061 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     6121 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagaaggaga
     6181 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     6241 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     6301 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatct
     6361 gctcatgttt gacagcttat catcgatgca taatgtgcct gtcaaatgga cgaagcaggg
     6421 attc
//
