LOCUS       sequence-471787-       10211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For overexpression of mEGFP-TAZ-CAMTA1-KS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Zhang Y, Stoppelkamp I, Fernandez-Pernas P, Allram M, Charman M, 
            Magalhaes AP, Piedavent-Salomon M, Sommer G, Sung YC, Meyer K, Grams
            N, Halko E, Dongre S, Meierhofer D, Malszycki M, Ilik IA, Aktas T, 
            Kraushar ML, Vastenhouw N, Weitzman MD, Grebien F, Niskanen H, Hnisz
            D
  TITLE     Probing condensate microenvironments with a micropeptide killswitch.
  JOURNAL   Nature. 2025 Jun 4. doi: 10.1038/s41586-025-09141-5.
  PUBMED    40468084
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Jun 4. doi: 10.1038/s41586-025-09141-5."
COMMENT     Sequence Label: pRK5_mEGFP-TAZ-CAMTA1-KS
FEATURES             Location/Qualifiers
     source          1..10211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      934..943
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(940..942,943..945,946..1656)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(985..1006)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1246..1265)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1593..1614
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      6511..6520
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6524..6658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6561..6580)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6615..6634
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6727..7084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6727..6918
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6750..6770)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6935..7070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7104..7120)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7113..7135)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7333..7788
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7420..7439)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7630..7651
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7805..7824)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7924..7946
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7984..8002)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8070..8174
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8175..8243,8244..9035)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8393..8412)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9206..9794
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9695..9714
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9948..9965
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10082..10103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10118..10123,10124..10141,10142..10148)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10156..10172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10161..10183
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10180..10196
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10180..10196
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcgtaccg gtcgccacca tggtgagcaa gggcgaggag
      961 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1021 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1081 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1141 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1201 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1261 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1321 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1381 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1441 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1501 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     1561 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1621 gccgggatca ctctcggcat ggacgagctg tacaagtgga tgaatccggc ctcggcgccc
     1681 cctccgctcc cgccgcctgg gcagcaagtg atccacgtca cgcaggacct agacacagac
     1741 ctcgaagccc tcttcaactc tgtcatgaat ccgaagccta gctcgtggcg gaagaagatc
     1801 ctgccggagt ctttctttaa ggagcctgat tcgggctcgc actcgcgcca gtccagcacc
     1861 gactcgtcgg gcggccaccc ggggcctcga ctggctgggg gtgcccagca tgtccgctcg
     1921 cactcgtcgc ccgcgtccct gcagctgggc accggcgcgg gtgctgcggg tagccccgcg
     1981 cagcagcacg cgcacctccg ccagcagtcc tacgacgtga ccgacgagct gccactgccc
     2041 ccgggctggg agatgacctt cacggccact ggccagaggt acttcctcaa tcacatagaa
     2101 aaaatcacca catggcaaga ccctaggaag gcgatgaatc agcctctgaa tcatatgaac
     2161 ctccaccctg ccgtcagttc cacaccagtg cctcagaggt ccatggcagt atcccagcca
     2221 aatctcggag ctggcggcag cgtgcatcac aagtgtaaca gcgccaaaca ccgcatcatc
     2281 tcgcccaagg tggagccacg gacagggggg tacgggagcc actcggaggt gcagcacaat
     2341 gacgtgtcgg agggcaagca cgagcacagc cacagcaagg gctccagccg tgagaagagg
     2401 aacggcaagg tggccaagcc cgtgctcctg caccagagca gcaccgaggt ctcctccacc
     2461 aaccaggtgg aagtccccga caccacccag agctcccctg tgtccatcag cagcgggctc
     2521 aacagcgacc cggacatggt ggacagcccg gtggtcacag gtgtgtccgg tatggcggtg
     2581 gcctctgtga tggggagctt gtcccagagc gccacggtgt tcatgtcaga ggtcaccaat
     2641 gaggccgtgt acaccatgtc ccccaccgct ggccccaacc accacctcct ctcacctgac
     2701 gcctctcagg gcctcgtcct ggccgtgagc tctgatggcc acaagttcgc ctttcccacc
     2761 acgggcagct cggagagcct gtccatgctg cccaccaacg tgtccgaaga gctggtcctc
     2821 tccaccaccc tcgacggtgg ccggaagatt ccagaaacca ccatgaactt tgaccccgac
     2881 tgtttcctta ataacccaaa gcagggccag acgtacgggg gtggaggcct gaaagccgag
     2941 atggtcagct ccaacatccg gcactcgcca cccggggagc ggagcttcag ctttaccacc
     3001 gtcctcacca aggagatcaa gaccgaggac acctccttcg agcagcagat ggccaaagaa
     3061 gcgtactcct cctccgcggc ggctgtggca gccagctccc tcaccctgac cgccggctcc
     3121 agcctcctgc cgtcgggcgg cggcctgagt cccagcacca ccctggagca gatggacttc
     3181 agcgccatcg actccaacaa ggactacacg tccagcttca gccagacggg ccacagcccc
     3241 cacatccacc agaccccctc cccgagcttc ttcctgcagg acgccagcaa acccctcccc
     3301 gtcgagcaga acacccacag cagcctgagt gactctgggg gcaccttcgt gatgcccacg
     3361 gtgaaaacgg aggcctcgtc ccaaaccagc tcctgcagcg gtcacgtgga gacgcggatc
     3421 gagtccactt cctccctcca cctcatgcag ttccaggcca acttccaggc catgacggca
     3481 gaaggggagg tcaccatgga gacctcgcag gcggcggaag ggagcgaggt cctgctcaag
     3541 tctggggagc tgcaggcttg cagctctgag cactacctgc agccggagac caacggggta
     3601 atccgaagcg ccggcggcgt ccccatcctc ccgggcaacg tggtgcaggg actctacccc
     3661 gtggcccagc ccagcctcgg caacgcctcc aacatggagc tcagcctgga ccactttgac
     3721 atctccttca gcaaccagtt ctccgacctg atcaacgact tcatctccgt ggaggggggc
     3781 agcagcacca tctatgggca ccagctggtg tcgggggaca gcacggcgct ctcacagtca
     3841 gaggacgggg cgcgggcccc cttcacccag gcagagatgt gcctcccctg ctgtagcccc
     3901 cagcagggta gcctgcagct gagcagctcg gagggcgggg ccagcaccat ggcctacatg
     3961 cacgtcgccg aggtggtctc ggccgcctcg gcccagggca ccctaggcat gctgcagcag
     4021 agcggacggg tgttcatggt gaccgactac tccccagagt ggtcttaccc agagggagga
     4081 gtgaaggtcc tcatcacagg cccgtggcaa gaagccagca ataactacag ctgcctgttt
     4141 gaccagatct cagtgcctgc atccctgatt cagcctgggg tgctgcgctg ctactgccca
     4201 gcccatgaca ctggtcttgt gaccctacaa gttgccttca acaaccagat catctccaac
     4261 tcggtggtgt ttgagtacaa agcccgggct ctgcccacgc tcccttcctc ccagcacgac
     4321 tggctgtcgt tggacgataa ccagttcagg atgtccatcc tggaacgact ggagcagatg
     4381 gagaggagga tggccgagat gacggggtcc cagcagcaca aacaggcgag cggaggcggc
     4441 agcagtggag gcggcagcgg gagcgggaat ggagggagcc aggcacagtg tgcttctggg
     4501 actggggcct tggggagctg ctttgagagc cgtgtggtcg tggtatgcga gaagatgatg
     4561 agccgagcct gctgggcgaa gtccaagcac ttgatccact caaagacttt ccgcggaatg
     4621 accctactcc acctggccgc tgcccagggc tatgccaccc taatccagac cctcatcaaa
     4681 tggcgtacaa agcacgcgga tagcattgac ctggaactgg aagttgaccc cttgaatgtg
     4741 gaccacttct cctgtactcc tctgatgtgg gcgtgtgccc tagggcactt ggaagctgcc
     4801 gtcgtgctgt acaagtggga ccgtcgggcc atctcgattc ccgactctct aggaaggctg
     4861 cctttgggaa ttgccaggtc acggggtcat gtgaaattag cagagtgtct ggagcacctg
     4921 cagagagatg agcaggctca gctgggacag aagcccagaa tccactgtcc tgcaagcgaa
     4981 gagcccagca cagagagctg gatggcccag tggcacagcg aagccatcag ctctccagaa
     5041 atacccaagg gagtcactgt tattgcaagc accaacccag agctgagaag acctcgttct
     5101 gaaccctcta attactacag cagtgagagc cacaaagatt atccggctcc caaaaagcat
     5161 aaattgaacc ctgagtactt ccagacaagg caggagaagc tgcttcccac tgcactgagt
     5221 ctggaagagc caaatatcag gaagcaaagc cctagttcta agcagtctgt ccccgagaca
     5281 ctcagcccca gtgaaggagt gagggacttc agccgggaac tctcccctcc cactccagag
     5341 actgcagcat ttcaagcctc tggatctcag cctgtaggaa agtggaattc caaagatctt
     5401 tacattggtg tgtctacagt acaggtgact ggaaatccga aggggaccag tgtaggaaag
     5461 gaggcagcac cttcacaggt gcgtccacgg gaaccaatga gtgtcctgat gatggctaac
     5521 agagaggtgg tgaatacaga gctggggtcc taccgtgata gtgcagaaaa tgaagaatgc
     5581 ggccagccca tggatgacat acaggtgaac atgatgacct tggcagaaca cattattgaa
     5641 gccacacctg accgaatcaa gcaggagaat tttgtgccca tggagtcctc aggattggaa
     5701 agaacagacc ctgccaccat tagcagtaca atgagctggc tggccagtta tctagcggat
     5761 gctgactgcc ttcccagtgc tgcccagatc cgaagtgcat ataacgagcc tctaacccct
     5821 tcttctaata ccagcttgag ccctgttggc tctcccgtca gtgaaatcgc tttcgagaaa
     5881 cctaaccttc cctccgccgc ggattggtca gaattcctga gtgcatctac cagtgagaag
     5941 gtagagaatg agtttgctca gctcactctg tctgatcatg aacagagaga actctatgag
     6001 gctgccaggc ttgtccagac agctttccgg aaatacaagg gccgaccctt gcgggaacag
     6061 caagaagtag ctgctgctgt tattcagcgt tgttacagaa aatataaaca gtacgcactt
     6121 tataaaaaga tgacacaggc tgccatcctt atccagagca aattccgaag ttactatgaa
     6181 caaaaaaaat tccagcagag ccgacgggct gctgtgctca tccaaaagta ctaccgaagt
     6241 tataagaaat gtggcaaaag acggcaggct cgccggacgg ctgtgattgt acaacagaaa
     6301 ctcaggagca gtttgctaac caaaaagcag gatcaagctg ctcgaaaaat aatgaggttt
     6361 cttcgccgct gtcgccacag ccccctggtg gaccataggc tgtacaaaag gagtgaaaga
     6421 attgaaaaag gccaaggaac tatgatgatg atgaataagt tggttctagc gcagttcttc
     6481 ttctcctgtc tatgaacctg cagaagcttg gccgccatgg cccaacttgt ttattgcagc
     6541 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6601 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcgg
     6661 gaattaattc ggcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg
     6721 taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6781 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6841 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6901 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6961 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7021 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7081 caaaaagctg ttaacagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7141 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7201 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7261 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     7321 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7381 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7441 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7501 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     7561 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7621 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     7681 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7741 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     7801 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7861 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7921 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7981 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     8041 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8101 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8161 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8221 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8281 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8341 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8401 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8461 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8521 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8581 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8641 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8701 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8761 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8821 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8881 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8941 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9001 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9061 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9121 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9181 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9241 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9301 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9361 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9421 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9481 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9541 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9601 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9661 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9721 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9781 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9841 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9901 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9961 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10021 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10081 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    10141 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
    10201 ttacgaatta a
//
