LOCUS       sequence-471817-        7206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hID1 response element in luciferase reporter assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGL4 [luc2P/hID1/Hygro] Vector
FEATURES             Location/Qualifiers
     source          1..7206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1331..1340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1337..2986
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             2990..3109
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    3161..3282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3172..3191)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3226..3245
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3476..3833
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3476..3667
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3499..3519)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3684..3819
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3746..3765
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3864..4901
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             4880..4888
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4925..4973
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5129..5146)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5300..5888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5380..5399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6088..6879,6880..6948))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    7053..7101
                     /note="synthetic polyadenylation signal"
     misc_feature    7115..7206
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7155..7174
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacca agcagatatt aaatattagg gctcagagag ggcatgcgac
       61 ccgcctgaat tcactcagct gcagagctgg aaagagaact caggcctttt tccccacgct
      121 ggaaggggta gctggggcgg agaggtgggg ggagcggtga agaaacccca agcggcccaa
      181 gctgtgggtc tgggttggga gactcgcagg tgtggggcgg ggaggtaagg tgacccttgc
      241 tcagcgaccg cgcccgcaag aaacgcattc ccaggcctcc cgcccggggt ctgcaggtga
      301 cgggctgggg ggagcacggg aactagctag accagtttgt cgtctccatg gcgaccgccc
      361 gcgcggcgcc agcctgacag tccgtccggg ttttatgaat gggtgacgtc acaggcctgg
      421 cgtctaacgg tctgagccgc tggttcagac gctgacacag accggcccgg gaagggaggg
      481 gggagactgt agctccgcag ctgccgcgcc gtgggaggga gaccctgctc tgaggtcttt
      541 gagaagaaaa tttaaaaagc agccaaaaat gggaaaaaac attaaaaaat cacgaactgt
      601 tgcaggttca ggaaattttt gcaaggagct gcaaattcaa ggtggaatcg aatgcagcct
      661 cactccactg cgctctatct agttcacttc ccagccaccc agccccaaac ttactagact
      721 ttcccgaatt aattgctccc acccgggagg gatctgggta ggccctccgg gtctcaggaa
      781 cacgaacagc aacattattt aggaattgag aaagcccagg ggagcagatg taaaggagcg
      841 cccgctttaa atttcggtcc tgatatttgt agatcagggt ccgagaagca tcttccaaga
      901 gggtccggaa acccaggctc tctgggctat ccagagccag ccgcttctcc gttcgggcct
      961 caatttctct catctgtgaa atggagctgg agaagtgaga aagtgaatat gggaaaaact
     1021 gattcctgag gtcttttcat tataaggcaa tgcctgctta gcttccttgc ctccttccag
     1081 aggagcccag tgcggccctc gcctctaccc gctgccctgc ccggtgccct gccaccgacc
     1141 cacccttgct gttctgaaac ccgggtcgtc tttccacact gcgagcaggc actagacgag
     1201 caggaggctg gacctaggag cgcgggtcac gcccccatgc cgcccattgg ctgcttttga
     1261 acgttctgag cccgcccctc cgggggccgt ggcgtgttta taagcttggc aatccggtac
     1321 tgttggtaaa gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta
     1381 cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct
     1441 ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga
     1501 gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa
     1561 ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc
     1621 cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct
     1681 gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa
     1741 gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa
     1801 gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg
     1861 cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat
     1921 catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc
     1981 ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac
     2041 cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta
     2101 cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg
     2161 cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt
     2221 cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg
     2281 cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg
     2341 catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg
     2401 ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga
     2461 cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc
     2521 catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga
     2581 cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt
     2641 ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga
     2701 gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga
     2761 cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga
     2821 gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg
     2881 tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat
     2941 ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgaatt ctcacggctt
     3001 ccctcccgag gtggaggagc aggccgccgg caccctgccc atgagctgcg cccaggagag
     3061 cggcatggat agacaccctg ctgcttgcgc cagcgccagg atcaacgtct aaggccgcga
     3121 ctctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt
     3181 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     3241 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     3301 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     3361 tacaaatgtg gtaaaatcga taaggatccg tttgcgtatt gggcgctctt ccgctgatct
     3421 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttagct accttctgag
     3481 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     3541 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     3601 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3661 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3721 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     3781 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcg
     3841 attcttctga cactagcgcc accatgaaga agcccgaact caccgctacc agcgttgaaa
     3901 aatttctcat cgagaagttc gacagtgtga gcgacctgat gcagttgtcg gagggcgaag
     3961 agagccgagc cttcagcttc gatgtcggcg gacgcggcta tgtactgcgg gtgaatagct
     4021 gcgctgatgg cttctacaaa gaccgctacg tgtaccgcca cttcgccagc gctgcactac
     4081 ccatccccga agtgttggac atcggcgagt tcagcgagag cctgacatac tgcatcagta
     4141 gacgcgccca aggcgttact ctccaagacc tccccgaaac agagctgcct gctgtgttac
     4201 agcctgtcgc cgaagctatg gatgctattg ccgccgccga cctcagtcaa accagcggct
     4261 tcggcccatt cgggccccaa ggcatcggcc agtacacaac ctggcgggat ttcatttgcg
     4321 ccattgctga tccccatgtc taccactggc agaccgtgat ggacgacacc gtgtccgcca
     4381 gcgtagctca agccctggac gaactgatgc tgtgggccga agactgtccc gaggtgcgcc
     4441 acctcgtcca tgccgacttc ggcagcaaca acgtcctgac cgacaacggc cgcatcaccg
     4501 ccgtaatcga ctggtccgaa gctatgttcg gggacagtca gtacgaggtg gccaacatct
     4561 tcttctggcg gccctggctg gcttgcatgg agcagcagac tcgctacttc gagcgccggc
     4621 atcccgagct ggccggcagc cctcgtctgc gagcctacat gctgcgcatc ggcctggatc
     4681 agctctacca gagcctcgtg gacggcaact tcgacgatgc tgcctgggct caaggccgct
     4741 gcgatgccat cgtccgcagc ggggccggca ccgtcggtcg cacacaaatc gctcgccgga
     4801 gcgcagccgt atggaccgac ggctgcgtcg aggtgctggc cgacagcggc aaccgccggc
     4861 ccagtacacg accgcgcgct aaggaggtag gtcgagttta aactctagaa ccggtcatgg
     4921 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgttcgaac
     4981 tagatgctgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     5041 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     5101 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5161 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5221 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5281 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5341 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5401 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5461 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5521 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5581 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5641 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5701 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5761 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5821 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5881 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5941 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6001 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg
     6061 gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc
     6121 agcgatctgc ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac
     6181 gattcgtgag ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc
     6241 accggccccc gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg
     6301 tcctgctact ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag
     6361 aagttcgcca gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc
     6421 acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac
     6481 atgatcaccc atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag
     6541 aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac
     6601 cgtcatgcca tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg
     6661 tgagtagtgt atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc
     6721 gccacatagc agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga
     6781 ctcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
     6841 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
     6901 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
     6961 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa
     7021 tattaaggta cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc
     7081 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
     7141 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
     7201 ttctct
//
