LOCUS       sequence-471821-        6082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Myc response element in luciferase reporter assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGL4 [luc2P/Myc/Hygro] Vector
FEATURES             Location/Qualifiers
     source          1..6082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        149..180
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      207..216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             213..1862
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1866..1985
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    2037..2158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2048..2067)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2102..2121
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2352..2709
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2352..2543
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2375..2395)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2560..2695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2622..2641
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2740..3777
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             3756..3764
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    3801..3849
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(4005..4022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4176..4764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4256..4275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4964..5755,5756..5824))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5929..5977
                     /note="synthetic polyadenylation signal"
     misc_feature    5991..6082
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6031..6050
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcagac cacgtggtca gaccacgtgg tcagaccacg
       61 tggtcagacc acgtggtcag accacgtggt cagaccacgt ggtcctcgag gatatcaaga
      121 tctggcctcg gcggccaagc ttagacacta gagggtatat aatggaagct cgacttccag
      181 cttggcaatc cggtactgtt ggtaaagcca ccatggaaga tgccaaaaac attaagaagg
      241 gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca
      301 tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat atcgaggtgg
      361 acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct atgaagcgct
      421 atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg cagttcttca
      481 tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac gacatctaca
      541 acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta ttcgtgagca
      601 agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata caaaagatca
      661 tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc ttcgtgactt
      721 cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc gaccgggaca
      781 aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag ggcgtagccc
      841 taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc ttcggcaacc
      901 agatcatccc cgacaccgct atcctcagcg tggtgccatt tcaccacggc ttcggcatgt
      961 tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg
     1021 aggagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg ctggtgccca
     1081 cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta agcaacttgc
     1141 acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac
     1201 gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc agcgccattc
     1261 tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg cccttcttcg
     1321 aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag cgcggcgagc
     1381 tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag gctacaaacg
     1441 ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg gacgaggacg
     1501 agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc taccaggtag
     1561 ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac gccggggtcg
     1621 ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg
     1681 gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt acaaccgcca
     1741 agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg accggcaagt
     1801 tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc aagatcgccg
     1861 tgaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc ctgcccatga
     1921 gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc gccaggatca
     1981 acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag acatgataag
     2041 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2101 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2161 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     2221 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtttg cgtattgggc
     2281 gctcttccgc tgatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg
     2341 ttagctacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     2401 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2461 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2521 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     2581 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     2641 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     2701 ttttgcaaaa agctcgattc ttctgacact agcgccacca tgaagaagcc cgaactcacc
     2761 gctaccagcg ttgaaaaatt tctcatcgag aagttcgaca gtgtgagcga cctgatgcag
     2821 ttgtcggagg gcgaagagag ccgagccttc agcttcgatg tcggcggacg cggctatgta
     2881 ctgcgggtga atagctgcgc tgatggcttc tacaaagacc gctacgtgta ccgccacttc
     2941 gccagcgctg cactacccat ccccgaagtg ttggacatcg gcgagttcag cgagagcctg
     3001 acatactgca tcagtagacg cgcccaaggc gttactctcc aagacctccc cgaaacagag
     3061 ctgcctgctg tgttacagcc tgtcgccgaa gctatggatg ctattgccgc cgccgacctc
     3121 agtcaaacca gcggcttcgg cccattcggg ccccaaggca tcggccagta cacaacctgg
     3181 cgggatttca tttgcgccat tgctgatccc catgtctacc actggcagac cgtgatggac
     3241 gacaccgtgt ccgccagcgt agctcaagcc ctggacgaac tgatgctgtg ggccgaagac
     3301 tgtcccgagg tgcgccacct cgtccatgcc gacttcggca gcaacaacgt cctgaccgac
     3361 aacggccgca tcaccgccgt aatcgactgg tccgaagcta tgttcgggga cagtcagtac
     3421 gaggtggcca acatcttctt ctggcggccc tggctggctt gcatggagca gcagactcgc
     3481 tacttcgagc gccggcatcc cgagctggcc ggcagccctc gtctgcgagc ctacatgctg
     3541 cgcatcggcc tggatcagct ctaccagagc ctcgtggacg gcaacttcga cgatgctgcc
     3601 tgggctcaag gccgctgcga tgccatcgtc cgcagcgggg ccggcaccgt cggtcgcaca
     3661 caaatcgctc gccggagcgc agccgtatgg accgacggct gcgtcgaggt gctggccgac
     3721 agcggcaacc gccggcccag tacacgaccg cgcgctaagg aggtaggtcg agtttaaact
     3781 ctagaaccgg tcatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt
     3841 ttttgtgtgt tcgaactaga tgctgtcgac cgatgccctt gagagccttc aacccagtca
     3901 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     3961 tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg
     4021 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4081 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4141 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4201 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4261 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4321 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4381 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4441 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4501 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4561 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4621 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4681 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4741 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4801 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4861 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4921 acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag tgcttgatca
     4981 gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc tgactccccg
     5041 tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca gcaatgatgc
     5101 cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca gcagggaggg
     5161 ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg agctgctgtc
     5221 gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg gccattgcta
     5281 ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct ggttcccagc
     5341 ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc tccttagggc
     5401 ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta atggcagcac
     5461 tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc ggcgagtact
     5521 caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta
     5581 tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc gggaatcgtt
     5641 cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg atatagccca
     5701 ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa
     5761 aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc
     5821 tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag tacgtctctc
     5881 aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa taaaatatct
     5941 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct
     6001 ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt
     6061 gcaggtgcca gaacatttct ct
//
