LOCUS       sequence-47190-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2220
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        37..55
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             81..146
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             153..173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactggctt actggcttat cgaaattaat acgactcact atagggagac
       61 ccaagctggc tagcaccatg gactacaaag accatgacgg tgattataaa gatcatgaca
      121 tcgattacaa ggatgacgat gacaagatgg cccccaagaa gaagaggaag gtgggcattc
      181 accgcggggt acctatggtg gacttgagga cactcggtta ttcgcaacag caacaggaga
      241 aaatcaagcc taaggtcagg agcaccgtcg cgcaacacca cgaggcgctt gtggggcatg
      301 gcttcactca tgcgcatatt gtcgcgcttt cacagcaccc tgcggcgctt gggacggtgg
      361 ctgtcaaata ccaagatatg attgcggccc tgcccgaagc cacgcacgag gcaattgtag
      421 gggtcggtaa acagtggtcg ggagcgcgag cacttgaggc gctgctgact gtggcgggtg
      481 agcttagggg gcctccgctc cagctcgaca ccgggcagct gctgaagatc gcgaagagag
      541 ggggagtaac agcggtagag gcagtgcacg cctggcgcaa tgcgctcacc ggggccccct
      601 tgaacctgac cccagaccag gtagtcgcaa tcgcgaacaa taatggggga aagcaagccc
      661 tggaaaccgt gcaaaggttg ttgccggtcc tttgtcaaga ccacggcctt acaccggagc
      721 aagtcgtggc cattgcatcc cacgacggtg gcaaacaggc tcttgagacg gttcagagac
      781 ttctcccagt tctctgtcaa gcccacggg
//
