LOCUS       sequence-471928-       11919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express GFP-FLAG-UL36USP (C65) - region 1-520 of UL36.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11919)
  AUTHORS   Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, 
            Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig 
            T, Dolken L, Lomonte P, Fachinetti D, Manel N
  TITLE     Centromeric DNA amplification triggered by viral proteins activates 
            nuclear cGAS.
  JOURNAL   Cell. 2025 May 29:S0092-8674(25)00556-2. doi: 
            10.1016/j.cell.2025.05.008.
  PUBMED    40460826
REFERENCE   2  (bases 1 to 11919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 29:S0092-8674(25)00556-2. doi: 10.1016/j.cell.2025.05.008."
COMMENT     Sequence Label: pTRIP-SFFV-EGFP-FLAG-UL36USP (C65)
FEATURES             Location/Qualifiers
     source          1..11919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3736..3745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3742..3744,3745..3747,3748..4458)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3742..3744,3745..3747,3748..4458)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3787..3808)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4048..4067)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4395..4416
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4468..4491
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6323..6503
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8031..8050)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8264..8283)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8383..8405
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8443..8461)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8529..8633
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8634..8702,8703..9494)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8852..8871)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9665..10253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10154..10173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10407..10424
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10494..10514)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10499..10828
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10679..10814
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcggcc gcgggctagc
     3721 ggatccccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     3781 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3841 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3901 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3961 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4021 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4081 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4141 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4201 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     4261 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4321 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4381 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4441 atggacgagc tgtacaagac tagtatggac tacaaagacg atgacgacaa gatgggtggc
     4501 ggaaacaaca ctaaccccgg gggtccggtc cataaacagg ccgggtctct ggccagcagg
     4561 gcacatatga tcgcgggcac cccaccgcac tccacgatgg aacgcggggg ggatcgcgac
     4621 atcgtggtca ccggtgctcg gaaccagttc gcgcccgacc tggagccggg ggggtcggta
     4681 tcgtgcatgc gctcgtcgct gtcctttctc agcctcatat ttgatgtggg ccctcgcgac
     4741 gtcctgtccg cggaggccat cgagggatgt ttggtcgagg ggggcgagtg gacgcgcgcg
     4801 accgcgggcc ctgggccgcc gcgcatgtgt tcgatcgtcg agctccccaa cttcctcgag
     4861 tacccagggg cgcgcggcgg actgcgctgt gtcttctcgc gcgtatacgg cgaggtgggc
     4921 ttcttcgggg agcccgcggc gggcctgctg gagacacaat gccccgcaca cacgttcttc
     4981 gccggcccgt gggccctgcg ccccctgtcg tacacgctcc taaccattgg ccccctaggg
     5041 atggggctgt tcagggacgg cgacaccgca tacctttttg acccgcacgg ccttccggag
     5101 ggcacccccg cgttcatcgc caaagtgcgg gcgggggaca tgtatccata cctgacgtat
     5161 tacacccgcg atcgcccgga cgtacggtgg gcgggagcca tggtgttttt cgtgccgtcg
     5221 ggcccggaac ccgcggctcc tgcggacttg acggccgcgg ctctgcatct ttacggggcc
     5281 agcgagactt acctgcagga cgaagcgttc agcgaacggc gcgtggccat cacgcacccc
     5341 ctgcggggcg agatcgcggg cctgggggag ccctgcgtcg gcgtgggccc ccgggagggg
     5401 gtagggggcc cggggccaca cccgcccaca gccgcccagt cgccgccacc gacccgggcc
     5461 cgtcgcgacg acagggcctc cgagacatcc cgggggacgg ccggtccgtc ggcaaaacca
     5521 gaggccaagc gcccgaatcg ggcgcccgac gatgtatggg cggtggccct gaagggtacc
     5581 ccacccacgg atcccccctc cgccgaccca ccctccgccg acccaccctc cgcgatccca
     5641 ccaccgcctc cctccgcccc caagaccccc gccgcagagg cggccgaaga agatgacgac
     5701 gacatgcggg tcctggagat gggcgtcgtc ccggttggtc ggcaccgggc acgctactcg
     5761 gccggccttc ccaagcgccg ccgacccacc tggactccgc cttccagcgt cgaagacctg
     5821 acttcggggg agaaaacgaa acgctcggcc ccccctgcca aaaccaagaa gaaatccacc
     5881 cccaaaggca aaacccccgt cggggccgcg gtccccgcct ccgttccgga gcctgtcctc
     5941 gcctcggcac cccccgaccc ggccgggccg ccggtcgccg aggcgggcga ggacgacggg
     6001 cccacggttc cggcgtcctc acaggccctc gaggcgctga agactcgccg ctgagtcgag
     6061 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     6121 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     6181 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6241 gactggaagg gctaattcac tcccaacgaa gacaaaatcg tcgagagatg ctgcatataa
     6301 gcagctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6361 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6421 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6481 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     6541 acttgcaaag aaatgaatat cagagagtga gaggccttga cattataata gatttagcag
     6601 gaattgaact aggagtggag cacacaggca aagctgcaga agtacttgga agaagccacc
     6661 agagatactc acgattctgc acatacctgg ctaatcccag atcctaagga ttacattaag
     6721 tttactaaca tttatataat gatttatagt ttaaagtata aacttatcta atttactatt
     6781 ctgacagata ttaattaatc ctcaaatatc ataagagatg attactatta tccccattta
     6841 acacaagagg aaactgagag ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc
     6901 gttagttacg actctatgat cttctgacac aaattccatt tactcctcac cctatgactc
     6961 agtcgaatat atcaaagtta tggacattat gctaagtaac aaattaccct tttatatagt
     7021 aaatactgag tagattgaga gaagaaattg tttgcaaacc tgaatagctt caagaagaag
     7081 agaagtgagg ataagaataa cagttgtcat ttaacaagtt ttaacaagta acttggttag
     7141 aaagggattc aaatgcataa agcaagggat aaatttttct ggcaacaaga ctatacaata
     7201 taaccttaaa tatgacttca aataattgtt ggaacttgat aaaactaatt aaatattatt
     7261 gaagattatc aatattataa atgtaattta cttttaaaaa gggaacatag aaatgtgtat
     7321 cattagagta gaaaacaatc cttattatca caatttgtca aaacaagttt gttattaaca
     7381 caagtagaat actgcattca attaagttga ctgcagattt tgtgttttgt taaaattaga
     7441 aagagataac aacaatttga attattgaaa gtaacatgta aatagttcta catacgttct
     7501 tttgacatct tgttcaatca ttgatcgaag ttctttatct tggaagaatt tgttccaaag
     7561 actctgaaat aaggaaaaca atctattata tagtctcaca cctttgtttt acttttagtg
     7621 atttcaattt aataatgtaa atggttaaaa tttattcttc tctgagatca tttcacattg
     7681 cagatagaaa acctgagact ggggtaattt ttattaaaat ctaatttaat ctcagaaaca
     7741 catctttatt ctaacatcaa tttttccagt ttgatattat catataaagt cagccttcct
     7801 catctgcagg ttccacaaca aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa
     7861 ttaaaaatag caatacaaca ataaaaaaat acaaatcaga aaaacagcac agtataacaa
     7921 ctttatttag catttacaat ctattaggta ttataagtaa tctagccaga tcctctacgc
     7981 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     8041 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     8101 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     8161 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     8221 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     8281 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     8341 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     8401 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     8461 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     8521 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8581 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8641 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8701 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8761 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8821 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8881 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8941 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9001 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9061 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9121 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9181 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9241 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9301 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9361 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9421 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9481 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9541 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9601 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9661 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9721 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9781 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9841 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9901 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9961 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10021 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10081 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10141 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10201 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10261 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10321 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10381 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    10441 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
    10501 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    10561 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
    10621 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
    10681 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    10741 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    10801 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
    10861 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
    10921 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
    10981 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
    11041 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
    11101 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
    11161 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
    11221 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
    11281 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
    11341 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
    11401 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
    11461 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
    11521 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
    11581 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
    11641 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
    11701 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
    11761 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
    11821 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
    11881 ccccggatct ttgtgaagga accttacttc tgtggtgtg
//
