LOCUS       sequence-471929-       12693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express tagRFP657-2A-coICP0 (co = codon-optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12693)
  AUTHORS   Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, 
            Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig 
            T, Dolken L, Lomonte P, Fachinetti D, Manel N
  TITLE     Centromeric DNA amplification triggered by viral proteins activates 
            nuclear cGAS.
  JOURNAL   Cell. 2025 May 29:S0092-8674(25)00556-2. doi: 
            10.1016/j.cell.2025.05.008.
  PUBMED    40460826
REFERENCE   2  (bases 1 to 12693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 29:S0092-8674(25)00556-2. doi: 10.1016/j.cell.2025.05.008."
COMMENT     Sequence Label: pTRIP-SFFV-tagRFP657-2A-coICP0
FEATURES             Location/Qualifiers
     source          1..12693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="far-red monomeric fluorescent protein with an 
                     emission peak at 657 nm (Morozova et al., 2010)"
                     /label=TagRFP657
                     /note="derived from mKate"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKV
                     VEGGPLPFAFDILATSFMYGSHTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEAHTEMLYPADGGLEGRTALALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDYRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             7097..7277
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8805..8824)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9038..9057)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9157..9179
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9217..9235)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9303..9407
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9408..9476,9477..10268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9626..9645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10439..11027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10928..10947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11181..11198
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11268..11288)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11273..11602
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11453..11588
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
     3781 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 cagagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
     3901 agcttcatgt acggcagtca caccttcatc aaccacaccc agggcatccc cgacttctgg
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggcccaca ccgagatgct gtaccccgct gacggcggcc tggaaggcag aaccgcgctg
     4201 gccctgaagc tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc
     4261 aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc cactagtgtc
     4501 gacgagccta gacccggagc cagcacaaga agacccgagg gaagacccca aagagaaccc
     4561 gcccccgacg tgtgggtctt cccttgtgat agggacctcc ccgatagcag cgattccgaa
     4621 gctgagacag aggtgggagg aagaggcgac gctgaccacc acgatgacga ctccgctagc
     4681 gaggccgatt ccacagatac cgagctcttc gagaccggac tgctgggccc tcaaggcgtc
     4741 gatggaggag ctgtgtccgg cggcagccct cctagagagg aagaccccgg aagctgtgga
     4801 ggcgctcctc ccagagagga cggaggaagc gacgaaggag atgtgtgcgc cgtgtgcaca
     4861 gacgagatcg cccctcatct gaggtgtgac acctttcctt gcatgcatag attctgcatc
     4921 ccttgcatga agacatggat gcagctcaga aacacatgcc ctctgtgtaa cgctaaactg
     4981 gtgtatctga tcgtcggagt gacacctagc ggctccttta gcaccattcc tatcgtcaac
     5041 gacccccaaa caagaatgga agctgaagaa gctgtgaggg ccggcacagc cgtggatttc
     5101 atctggaccg gcaatcagag gtttgctcct agatatctga ccctcggagg acacacagtg
     5161 agggctctgt cccctacaca ccccgagcct acaacagacg aagacgacga cgacctcgat
     5221 gatgccgact acgtgccccc cgcccctagg agaacaccta gagctcctcc taggagaggc
     5281 gctgccgctc ctcccgtgac cggcggagcc agccatgctg cccctcaacc cgccgctgct
     5341 aggacagctc cccctagcgc tcccattgga ccccacggaa gcagcaacac aaacaccacc
     5401 accaatagct ccggaggcgg cggcagcaga caaagcagag ctgccgcccc tagaggagct
     5461 tccggaccta gcggaggagt cggagtcgga gtgggagtgg tggaggccga agccggaagg
     5521 cccagaggaa gaaccggacc tctggtgaac agacccgctc ctctcgctaa caatagagac
     5581 cccattgtga tcagcgacag cccccccgcc tcccctcata ggcctcccgc cgctcctatg
     5641 cccggcagcg cccctaggcc cggccctccc gccagcgctg ctgccagcgg acccgccaga
     5701 cctagggctg ctgtcgctcc ttgcgtgaga gcccctcctc ccggccccgg acctagggcc
     5761 cccgcccccg gagctgaacc cgccgccaga cccgccgatg ctagaagagt gccccagtcc
     5821 catagctctc tggctcaagc tgccaatcaa gagcaatctc tgtgcagagc tagggctaca
     5881 gtcgctagag gcagcggagg acccggcgtg gaaggcggac acggacctag cagaggagct
     5941 gctccttccg gagccgctcc tctgccttcc gccgctagcg tggagcaaga agccgccgtg
     6001 aggcctagaa agagaagggg ctccggccaa gagaacccca gccctcagtc cacaagacct
     6061 cccctcgctc ccgccggcgc taaaagagcc gccacacacc ctcctagcga ttccggcccc
     6121 ggaggcagag gccaaggcgg acccggaaca cctctgacat cctccgccgc ttccgctagc
     6181 tccagctccg ccagctccag cagcgctcct acacccgctg gagccgctag cagcgctgcc
     6241 ggagctgcta gcagcagcgc tagcgcttcc agcggaggag ctgtcggagc cctcggagga
     6301 agacaagagg aaacctctct gggacctaga gccgctagcg gccctagggg ccccagaaag
     6361 tgcgctagaa agacaagaca tgctgagacc agcggcgctg tgcccgccgg aggactgaca
     6421 aggtatctgc ctatcagcgg agtcagcagc gtggtggctc tgagccccta cgtgaacaaa
     6481 accatcaccg gagattgtct gcccatcctc gacatggaga ccggaaacat tggcgcctat
     6541 gtggtgctgg tggatcagac cggcaacatg gctaccagac tgagagctgc cgtgcccggc
     6601 tggtctagaa ggacactcct ccccgagaca gccggaaacc acgtgatgcc tcccgagtac
     6661 cccacagccc ccgctagcga atggaatagc ctctggatga cacccgtggg caatatgctg
     6721 ttcgaccaag gaaccctcgt cggcgctctg gattttagat ctctgaggag cagacatcct
     6781 tggtccggcg agcaaggcgc ttccacaaga gacgagggaa agcagtgact cgagacctag
     6841 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     6901 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     6961 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7021 aagggctaat tcactcccaa cgaagacaaa atcgtcgaga gatgctgcat ataagcagct
     7081 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7141 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7201 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7261 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7321 aaagaaatga atatcagaga gtgagaggcc ttgacattat aatagattta gcaggaattg
     7381 aactaggagt ggagcacaca ggcaaagctg cagaagtact tggaagaagc caccagagat
     7441 actcacgatt ctgcacatac ctggctaatc ccagatccta aggattacat taagtttact
     7501 aacatttata taatgattta tagtttaaag tataaactta tctaatttac tattctgaca
     7561 gatattaatt aatcctcaaa tatcataaga gatgattact attatcccca tttaacacaa
     7621 gaggaaactg agagggaaag atgttgaagt aattttccca caattacagc atccgttagt
     7681 tacgactcta tgatcttctg acacaaattc catttactcc tcaccctatg actcagtcga
     7741 atatatcaaa gttatggaca ttatgctaag taacaaatta cccttttata tagtaaatac
     7801 tgagtagatt gagagaagaa attgtttgca aacctgaata gcttcaagaa gaagagaagt
     7861 gaggataaga ataacagttg tcatttaaca agttttaaca agtaacttgg ttagaaaggg
     7921 attcaaatgc ataaagcaag ggataaattt ttctggcaac aagactatac aatataacct
     7981 taaatatgac ttcaaataat tgttggaact tgataaaact aattaaatat tattgaagat
     8041 tatcaatatt ataaatgtaa tttactttta aaaagggaac atagaaatgt gtatcattag
     8101 agtagaaaac aatccttatt atcacaattt gtcaaaacaa gtttgttatt aacacaagta
     8161 gaatactgca ttcaattaag ttgactgcag attttgtgtt ttgttaaaat tagaaagaga
     8221 taacaacaat ttgaattatt gaaagtaaca tgtaaatagt tctacatacg ttcttttgac
     8281 atcttgttca atcattgatc gaagttcttt atcttggaag aatttgttcc aaagactctg
     8341 aaataaggaa aacaatctat tatatagtct cacacctttg ttttactttt agtgatttca
     8401 atttaataat gtaaatggtt aaaatttatt cttctctgag atcatttcac attgcagata
     8461 gaaaacctga gactggggta atttttatta aaatctaatt taatctcaga aacacatctt
     8521 tattctaaca tcaatttttc cagtttgata ttatcatata aagtcagcct tcctcatctg
     8581 caggttccac aacaaaaatc caaccaactg tggatcaaaa atattgggaa aaaattaaaa
     8641 atagcaatac aacaataaaa aaatacaaat cagaaaaaca gcacagtata acaactttat
     8701 ttagcattta caatctatta ggtattataa gtaatctagc cagatcctct acgccggacg
     8761 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     8821 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     8881 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     8941 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     9001 gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat
     9061 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     9121 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     9181 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     9241 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     9301 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     9361 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     9421 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     9481 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9541 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9601 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     9661 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9721 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9781 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9841 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9901 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9961 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    10021 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    10081 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    10141 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    10201 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    10261 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    10321 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    10381 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    10441 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    10501 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10561 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    10621 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10681 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10741 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10801 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10861 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10921 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10981 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    11041 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    11101 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    11161 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    11221 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
    11281 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    11341 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    11401 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    11461 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    11521 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    11581 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
    11641 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
    11701 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
    11761 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
    11821 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
    11881 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
    11941 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
    12001 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
    12061 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
    12121 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
    12181 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
    12241 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
    12301 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
    12361 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
    12421 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
    12481 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
    12541 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
    12601 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
    12661 atctttgtga aggaacctta cttctgtggt gtg
//
