LOCUS       sequence-471934-       12579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express tagBFP-2A-ICP0-FXE (ICP0 ∆106-149).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12579)
  AUTHORS   Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, 
            Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig 
            T, Dolken L, Lomonte P, Fachinetti D, Manel N
  TITLE     Centromeric DNA amplification triggered by viral proteins activates 
            nuclear cGAS.
  JOURNAL   Cell. 2025 May 29:S0092-8674(25)00556-2. doi: 
            10.1016/j.cell.2025.05.008.
  PUBMED    40460826
REFERENCE   2  (bases 1 to 12579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 29:S0092-8674(25)00556-2. doi: 10.1016/j.cell.2025.05.008."
COMMENT     Sequence Label: pTRIP-SFFV-tagBFP-2A-ICP0-FxE
FEATURES             Location/Qualifiers
     source          1..12579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             6983..7163
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8691..8710)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8924..8943)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9043..9065
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9103..9121)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9189..9293
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9294..9362,9363..10154)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9512..9531)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10325..10913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10814..10833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11067..11084
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11154..11174)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11159..11488
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11339..11474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc catggagccc
     4501 cgccccggag cgagtacccg ccggcctgag ggccgccccc agcgcgagcc cgccccggat
     4561 gtctgggtgt ttccctgcga ccgagacctg ccggacagca gcgactctga ggcggagacc
     4621 gaagtggggg ggcgggggga cgccgaccac catgacgacg actccgcctc cgaggcggac
     4681 agcacggaca cggaactgtt cgagacgggg ctgctggggc cgcagggcgt ggatgggggg
     4741 gcggtctcgg gggggagccc cccccgcgag gaagaccccg gcagttgcgg gggcgccccc
     4801 cctcgaggaa ttccaattcg gggcaacacc tgcccgctgt gcaacgccaa gctggtgtac
     4861 ctgatagtgg gcgtgacgcc cagcgggtcg ttcagcacca tcccgatcgt gaacgacccc
     4921 cagacccgca tggaggccga ggaggccgtc agggcgggca cggccgtgga ctttatctgg
     4981 acgggcaatc agcggttcgc cccgcggtac ctgaccctgg gggggcacac ggtgagggcc
     5041 ctgtcgccca cccacccgga gcccaccacg gacgaggatg acgacgacct ggacgacgca
     5101 gactacgtac cgcccgcccc ccgccggacg ccccgcgccc ccccacgcag aggcgccgcc
     5161 gcgccccccg tgacgggcgg ggcgtctcac gcagcccccc agccggccgc ggctcggaca
     5221 gcgcccccct cggcgcccat cgggccacac ggcagcagta acaccaacac caccaccaac
     5281 agcagcggcg gcggcggctc ccgccagtcg cgagccgcgg cgccgcgggg ggcgtctggc
     5341 ccctccgggg gggttggggt tggggttggg gttgttgaag cggaggcggg gcggccgagg
     5401 ggccggacgg gcccccttgt caacagaccc gccccccttg caaacaacag agaccccata
     5461 gtgatcagcg actccccccc ggcctctccc cacaggcccc ccgcggcgcc catgccaggc
     5521 tccgcccccc gccccgggcc ccccgcgtcc gcggccgcgt cgggacccgc gcgcccccgc
     5581 gcggccgtgg ccccgtgcgt gcgagcgccg cctccggggc ccggcccccg cgccccggcc
     5641 cccggggcgg agccggccgc ccgccccgcg gacgcgcgcc gtgtgcccca gtcgcactcg
     5701 tccctggctc aggccgcgaa ccaagaacag agtctgtgcc gggcgcgtgc gacggtggcg
     5761 cgcggctcgg gggggccggg cgtggagggt gggcacgggc cctcccgcgg cgccgccccc
     5821 tccggcgccg ccccgctccc ctccgccgcc tctgtcgagc aggaggcggc ggtgcgtccg
     5881 aggaagaggc gcgggtcggg ccaggaaaac ccctcccccc agtccacgcg tccccccctc
     5941 gcgccggcag gggccaagag ggcggcgacg caccccccct ccgactcagg gccggggggg
     6001 cgcggccagg gtgggcccgg gacccccctg acgtcctcgg cggcctccgc ctcttcctcc
     6061 tctgcctctt cctcctcggc cccgaccccc gcgggggccg cctcttccgc cgccggggcc
     6121 gcgtcctcct ccgcttccgc ctcctcgggc ggggccgtcg gtgccctggg agggagacaa
     6181 gaggaaacct ccctcggccc ccgcgctgct tctgggccgc gggggccgag gaagtgtgcc
     6241 cggaagacgc gccacgcgga gacttccggg gccgtccccg cgggcggcct cacgcgctac
     6301 ctgcccatct cgggggtctc tagcgtggtc gccctgtcgc cttacgtgaa caagactatc
     6361 acgggggact gcctgcccat cctggacatg gagacgggga acatcggggc gtacgtggtc
     6421 ctggtggacc agacgggaaa catggcgacc cggctgcggg ccgcggtccc cggctggagc
     6481 cgccgcaccc tgctccccga gaccgcgggt aaccacgtga tgccccccga gtacccgacg
     6541 gcccccgcgt cggagtggaa cagcctctgg atgacccccg tggggaacat gctgttcgac
     6601 cagggcaccc tagtgggcgc cctggacttc cgcagcctgc ggtctcggca cccgtggtcc
     6661 ggggagcagg gggcgtcgac ccgggacgag ggaaaacaat aaactagtgt cgacctcgag
     6721 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     6781 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     6841 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6901 gactggaagg gctaattcac tcccaacgaa gacaaaatcg tcgagagatg ctgcatataa
     6961 gcagctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7021 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7081 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7141 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7201 acttgcaaag aaatgaatat cagagagtga gaggccttga cattataata gatttagcag
     7261 gaattgaact aggagtggag cacacaggca aagctgcaga agtacttgga agaagccacc
     7321 agagatactc acgattctgc acatacctgg ctaatcccag atcctaagga ttacattaag
     7381 tttactaaca tttatataat gatttatagt ttaaagtata aacttatcta atttactatt
     7441 ctgacagata ttaattaatc ctcaaatatc ataagagatg attactatta tccccattta
     7501 acacaagagg aaactgagag ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc
     7561 gttagttacg actctatgat cttctgacac aaattccatt tactcctcac cctatgactc
     7621 agtcgaatat atcaaagtta tggacattat gctaagtaac aaattaccct tttatatagt
     7681 aaatactgag tagattgaga gaagaaattg tttgcaaacc tgaatagctt caagaagaag
     7741 agaagtgagg ataagaataa cagttgtcat ttaacaagtt ttaacaagta acttggttag
     7801 aaagggattc aaatgcataa agcaagggat aaatttttct ggcaacaaga ctatacaata
     7861 taaccttaaa tatgacttca aataattgtt ggaacttgat aaaactaatt aaatattatt
     7921 gaagattatc aatattataa atgtaattta cttttaaaaa gggaacatag aaatgtgtat
     7981 cattagagta gaaaacaatc cttattatca caatttgtca aaacaagttt gttattaaca
     8041 caagtagaat actgcattca attaagttga ctgcagattt tgtgttttgt taaaattaga
     8101 aagagataac aacaatttga attattgaaa gtaacatgta aatagttcta catacgttct
     8161 tttgacatct tgttcaatca ttgatcgaag ttctttatct tggaagaatt tgttccaaag
     8221 actctgaaat aaggaaaaca atctattata tagtctcaca cctttgtttt acttttagtg
     8281 atttcaattt aataatgtaa atggttaaaa tttattcttc tctgagatca tttcacattg
     8341 cagatagaaa acctgagact ggggtaattt ttattaaaat ctaatttaat ctcagaaaca
     8401 catctttatt ctaacatcaa tttttccagt ttgatattat catataaagt cagccttcct
     8461 catctgcagg ttccacaaca aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa
     8521 ttaaaaatag caatacaaca ataaaaaaat acaaatcaga aaaacagcac agtataacaa
     8581 ctttatttag catttacaat ctattaggta ttataagtaa tctagccaga tcctctacgc
     8641 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     8701 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     8761 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     8821 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     8881 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     8941 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     9001 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     9061 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     9121 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     9181 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9241 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9301 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9361 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9421 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9481 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9541 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9601 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9661 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9721 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9781 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9841 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9901 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9961 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    10021 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    10081 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    10141 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    10201 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10441 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    11041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    11101 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
    11161 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    11221 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
    11281 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
    11341 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    11401 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    11461 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
    11521 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
    11581 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
    11641 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
    11701 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
    11761 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
    11821 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
    11881 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
    11941 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
    12001 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
    12061 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
    12121 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
    12181 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
    12241 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
    12301 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
    12361 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
    12421 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
    12481 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
    12541 ccccggatct ttgtgaagga accttacttc tgtggtgtg
//
