LOCUS       sequence-471936-       11253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express tagBFP-2A-FLAG-coK8 (co = codon-optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, 
            Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig 
            T, Dolken L, Lomonte P, Fachinetti D, Manel N
  TITLE     Centromeric DNA amplification triggered by viral proteins activates 
            nuclear cGAS.
  JOURNAL   Cell. 2025 May 29:S0092-8674(25)00556-2. doi: 
            10.1016/j.cell.2025.05.008.
  PUBMED    40460826
REFERENCE   2  (bases 1 to 11253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 29:S0092-8674(25)00556-2. doi: 10.1016/j.cell.2025.05.008."
COMMENT     Sequence Label: pTRIP-SFFV-tagBFP-2A-FLAG-coK8
FEATURES             Location/Qualifiers
     source          1..11253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      4498..4507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4507..4530
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             5657..5837
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7365..7384)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7598..7617)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7717..7739
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7777..7795)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7863..7967
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7968..8036,8037..8828)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8186..8205)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8999..9587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9488..9507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9741..9758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9828..9848)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9833..10162
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10013..10148
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc cactagtgcc
     4501 accatggatt ataaggatga cgatgacaag cctcggatga aagatatccc cacaaaatcc
     4561 tcaccaggta ccgataacag cgaaaaggac gaggcggtga tcgaagagga cctgtctctg
     4621 aatggacagc cgttcttcac agataacacg gatggtggag agaatgaggt atcctggact
     4681 tcttctctcc tgagcacata tgtggggtgt caaccacccg cgatccccgt gtgcgagacc
     4741 gtgatcgatc tgacggcacc aagccagagc ggtgccccag gcgatgagca tctcccttgt
     4801 tctcttaacg ccgagacaaa gtttcatatt ccagacccaa gctggacact gagccatacc
     4861 ccgccccggg gacctcatat cagccagcaa ctccctacca ggcgctctaa acgtcggctg
     4921 caccggaaat tcgaggaaga gagactgtgt accaaagcta agcaaggggc cgggagacct
     4981 gttccggctt cagtggtgaa agtcggcaat attacaccac actacggaga ggagcttact
     5041 cgcggagatg cagtgcccgc tgctcccatc actccgccgt atccaagggt gcagcgtcct
     5101 gctcaaccta cgcacgtact cttcagcccc gtgttcgtga gcctgaaagc tgaggtctgc
     5161 gaccaaagcc acagcccaac caggaaacag ggtcgctatg gacgagttag tagcaaggcc
     5221 tatacccgac aacttcaaca agcgttggaa gagaaagacg cccagctttg cttcttagct
     5281 gctcgcctcg aagcccacaa agagcaaatc atctttctgc gagatatgtt gatgcgcatg
     5341 tgtcaacaac ccgccagccc cactgatgct cctctgccac cttgctaact cgagacctag
     5401 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     5461 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     5521 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     5581 aagggctaat tcactcccaa cgaagacaaa atcgtcgaga gatgctgcat ataagcagct
     5641 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5701 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5761 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5821 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     5881 aaagaaatga atatcagaga gtgagaggcc ttgacattat aatagattta gcaggaattg
     5941 aactaggagt ggagcacaca ggcaaagctg cagaagtact tggaagaagc caccagagat
     6001 actcacgatt ctgcacatac ctggctaatc ccagatccta aggattacat taagtttact
     6061 aacatttata taatgattta tagtttaaag tataaactta tctaatttac tattctgaca
     6121 gatattaatt aatcctcaaa tatcataaga gatgattact attatcccca tttaacacaa
     6181 gaggaaactg agagggaaag atgttgaagt aattttccca caattacagc atccgttagt
     6241 tacgactcta tgatcttctg acacaaattc catttactcc tcaccctatg actcagtcga
     6301 atatatcaaa gttatggaca ttatgctaag taacaaatta cccttttata tagtaaatac
     6361 tgagtagatt gagagaagaa attgtttgca aacctgaata gcttcaagaa gaagagaagt
     6421 gaggataaga ataacagttg tcatttaaca agttttaaca agtaacttgg ttagaaaggg
     6481 attcaaatgc ataaagcaag ggataaattt ttctggcaac aagactatac aatataacct
     6541 taaatatgac ttcaaataat tgttggaact tgataaaact aattaaatat tattgaagat
     6601 tatcaatatt ataaatgtaa tttactttta aaaagggaac atagaaatgt gtatcattag
     6661 agtagaaaac aatccttatt atcacaattt gtcaaaacaa gtttgttatt aacacaagta
     6721 gaatactgca ttcaattaag ttgactgcag attttgtgtt ttgttaaaat tagaaagaga
     6781 taacaacaat ttgaattatt gaaagtaaca tgtaaatagt tctacatacg ttcttttgac
     6841 atcttgttca atcattgatc gaagttcttt atcttggaag aatttgttcc aaagactctg
     6901 aaataaggaa aacaatctat tatatagtct cacacctttg ttttactttt agtgatttca
     6961 atttaataat gtaaatggtt aaaatttatt cttctctgag atcatttcac attgcagata
     7021 gaaaacctga gactggggta atttttatta aaatctaatt taatctcaga aacacatctt
     7081 tattctaaca tcaatttttc cagtttgata ttatcatata aagtcagcct tcctcatctg
     7141 caggttccac aacaaaaatc caaccaactg tggatcaaaa atattgggaa aaaattaaaa
     7201 atagcaatac aacaataaaa aaatacaaat cagaaaaaca gcacagtata acaactttat
     7261 ttagcattta caatctatta ggtattataa gtaatctagc cagatcctct acgccggacg
     7321 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     7381 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     7441 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     7501 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     7561 gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat
     7621 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     7681 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7741 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     7801 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7861 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7921 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7981 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8041 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8101 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8161 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8221 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8281 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8341 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8401 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8461 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8521 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8581 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8641 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8701 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8761 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8821 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8881 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8941 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9001 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9061 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9121 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9361 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9421 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9481 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9541 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9601 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9661 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9721 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9781 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
     9841 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9901 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9961 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    10021 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    10081 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    10141 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
    10201 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
    10261 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
    10321 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
    10381 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
    10441 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
    10501 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
    10561 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
    10621 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
    10681 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
    10741 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
    10801 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
    10861 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
    10921 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
    10981 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
    11041 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
    11101 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
    11161 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
    11221 atctttgtga aggaacctta cttctgtggt gtg
//
