LOCUS       sequence-471940-       11844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express GFP-FLAG-UL36USP (C40) - region 25-520 of UL36.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11844)
  AUTHORS   Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, 
            Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig 
            T, Dolken L, Lomonte P, Fachinetti D, Manel N
  TITLE     Centromeric DNA amplification triggered by viral proteins activates 
            nuclear cGAS.
  JOURNAL   Cell. 2025 May 29:S0092-8674(25)00556-2. doi: 
            10.1016/j.cell.2025.05.008.
  PUBMED    40460826
REFERENCE   2  (bases 1 to 11844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 29:S0092-8674(25)00556-2. doi: 10.1016/j.cell.2025.05.008."
COMMENT     Sequence Label: pTRIP-SFFV-EGFP-FLAG-UL36USP (C40)
FEATURES             Location/Qualifiers
     source          1..11844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3736..3745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3742..3744,3745..3747,3748..4458)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3742..3744,3745..3747,3748..4458)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3787..3808)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4048..4067)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4395..4416
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4468..4491
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6248..6428
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7956..7975)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8189..8208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8308..8330
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8368..8386)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8454..8558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8559..8627,8628..9419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8777..8796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9590..10178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10079..10098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10332..10349
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10419..10439)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10424..10753
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10604..10739
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcggcc gcgggctagc
     3721 ggatccccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     3781 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3841 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3901 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3961 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4021 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4081 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4141 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4201 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     4261 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4321 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4381 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4441 atggacgagc tgtacaagac tagtatggac tacaaagacg atgacgacaa gatgatcgcg
     4501 ggcaccccac cgcactccac gatggaacgc gggggggatc gcgacatcgt ggtcaccggt
     4561 gctcggaacc agttcgcgcc cgacctggag ccgggggggt cggtatcgtg catgcgctcg
     4621 tcgctgtcct ttctcagcct catatttgat gtgggccctc gcgacgtcct gtccgcggag
     4681 gccatcgagg gatgtttggt cgaggggggc gagtggacgc gcgcgaccgc gggccctggg
     4741 ccgccgcgca tgtgttcgat cgtcgagctc cccaacttcc tcgagtaccc aggggcgcgc
     4801 ggcggactgc gctgtgtctt ctcgcgcgta tacggcgagg tgggcttctt cggggagccc
     4861 gcggcgggcc tgctggagac acaatgcccc gcacacacgt tcttcgccgg cccgtgggcc
     4921 ctgcgccccc tgtcgtacac gctcctaacc attggccccc tagggatggg gctgttcagg
     4981 gacggcgaca ccgcatacct ttttgacccg cacggccttc cggagggcac ccccgcgttc
     5041 atcgccaaag tgcgggcggg ggacatgtat ccatacctga cgtattacac ccgcgatcgc
     5101 ccggacgtac ggtgggcggg agccatggtg tttttcgtgc cgtcgggccc ggaacccgcg
     5161 gctcctgcgg acttgacggc cgcggctctg catctttacg gggccagcga gacttacctg
     5221 caggacgaag cgttcagcga acggcgcgtg gccatcacgc accccctgcg gggcgagatc
     5281 gcgggcctgg gggagccctg cgtcggcgtg ggcccccggg agggggtagg gggcccgggg
     5341 ccacacccgc ccacagccgc ccagtcgccg ccaccgaccc gggcccgtcg cgacgacagg
     5401 gcctccgaga catcccgggg gacggccggt ccgtcggcaa aaccagaggc caagcgcccg
     5461 aatcgggcgc ccgacgatgt atgggcggtg gccctgaagg gtaccccacc cacggatccc
     5521 ccctccgccg acccaccctc cgccgaccca ccctccgcga tcccaccacc gcctccctcc
     5581 gcccccaaga cccccgccgc agaggcggcc gaagaagatg acgacgacat gcgggtcctg
     5641 gagatgggcg tcgtcccggt tggtcggcac cgggcacgct actcggccgg ccttcccaag
     5701 cgccgccgac ccacctggac tccgccttcc agcgtcgaag acctgacttc gggggagaaa
     5761 acgaaacgct cggccccccc tgccaaaacc aagaagaaat ccacccccaa aggcaaaacc
     5821 cccgtcgggg ccgcggtccc cgcctccgtt ccggagcctg tcctcgcctc ggcacccccc
     5881 gacccggccg ggccgccggt cgccgaggcg ggcgaggacg acgggcccac ggttccggcg
     5941 tcctcacagg ccctcgaggc gctgaagact cgccgctgag tcgagaccta gaaaaacatg
     6001 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     6061 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     6121 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6181 ttcactccca acgaagacaa aatcgtcgag agatgctgca tataagcagc tgctttttgc
     6241 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6301 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6361 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6421 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     6481 aatatcagag agtgagaggc cttgacatta taatagattt agcaggaatt gaactaggag
     6541 tggagcacac aggcaaagct gcagaagtac ttggaagaag ccaccagaga tactcacgat
     6601 tctgcacata cctggctaat cccagatcct aaggattaca ttaagtttac taacatttat
     6661 ataatgattt atagtttaaa gtataaactt atctaattta ctattctgac agatattaat
     6721 taatcctcaa atatcataag agatgattac tattatcccc atttaacaca agaggaaact
     6781 gagagggaaa gatgttgaag taattttccc acaattacag catccgttag ttacgactct
     6841 atgatcttct gacacaaatt ccatttactc ctcaccctat gactcagtcg aatatatcaa
     6901 agttatggac attatgctaa gtaacaaatt acccttttat atagtaaata ctgagtagat
     6961 tgagagaaga aattgtttgc aaacctgaat agcttcaaga agaagagaag tgaggataag
     7021 aataacagtt gtcatttaac aagttttaac aagtaacttg gttagaaagg gattcaaatg
     7081 cataaagcaa gggataaatt tttctggcaa caagactata caatataacc ttaaatatga
     7141 cttcaaataa ttgttggaac ttgataaaac taattaaata ttattgaaga ttatcaatat
     7201 tataaatgta atttactttt aaaaagggaa catagaaatg tgtatcatta gagtagaaaa
     7261 caatccttat tatcacaatt tgtcaaaaca agtttgttat taacacaagt agaatactgc
     7321 attcaattaa gttgactgca gattttgtgt tttgttaaaa ttagaaagag ataacaacaa
     7381 tttgaattat tgaaagtaac atgtaaatag ttctacatac gttcttttga catcttgttc
     7441 aatcattgat cgaagttctt tatcttggaa gaatttgttc caaagactct gaaataagga
     7501 aaacaatcta ttatatagtc tcacaccttt gttttacttt tagtgatttc aatttaataa
     7561 tgtaaatggt taaaatttat tcttctctga gatcatttca cattgcagat agaaaacctg
     7621 agactggggt aatttttatt aaaatctaat ttaatctcag aaacacatct ttattctaac
     7681 atcaattttt ccagtttgat attatcatat aaagtcagcc ttcctcatct gcaggttcca
     7741 caacaaaaat ccaaccaact gtggatcaaa aatattggga aaaaattaaa aatagcaata
     7801 caacaataaa aaaatacaaa tcagaaaaac agcacagtat aacaacttta tttagcattt
     7861 acaatctatt aggtattata agtaatctag ccagatcctc tacgccggac gcatcgtggc
     7921 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     7981 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     8041 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     8101 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     8161 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8221 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     8281 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     8341 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     8401 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8461 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8521 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8581 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8641 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8701 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8761 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8821 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8881 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8941 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9001 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9061 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9121 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9181 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9241 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9301 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9361 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9421 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9481 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9541 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9601 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9661 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9721 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9781 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9841 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9901 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9961 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10021 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10081 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10141 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10201 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10261 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10321 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    10381 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
    10441 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    10501 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    10561 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    10621 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10681 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10741 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
    10801 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
    10861 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
    10921 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
    10981 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
    11041 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
    11101 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
    11161 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
    11221 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
    11281 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
    11341 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
    11401 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
    11461 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
    11521 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
    11581 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
    11641 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
    11701 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
    11761 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
    11821 aaggaacctt acttctgtgg tgtg
//
