LOCUS       sequence-471945-        9363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lbr mCherry fusion protein without MCP domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Bhat P, Chow A, Emert B, Ettlin O, Quinodoz SA, Strehle M, Takei Y, 
            Burr A, Goronzy IN, Chen AW, Huang W, Ferrer JLM, Soehalim E, Goh 
            ST, Chari T, Sullivan DK, Blanco MR, Guttman M
  TITLE     Genome organization around nuclear speckles drives mRNA splicing 
            efficiency.
  JOURNAL   Nature. 2024 May;629(8014):1165-1173. doi: 
            10.1038/s41586-024-07429-6. Epub 2024 May 8.
  PUBMED    38720076
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07429-6"; journalName: "Nature"; date: 
            "2024-05"; volume: "629"; issue: "8014"; pages: "1165-1173"
COMMENT     Sequence Label: pCAG-SpyTag-Flag-Lbr-V5-mCherry
FEATURES             Location/Qualifiers
     source          1..9363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(14..34)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(44..82)
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     primer_bind     complement(128..147)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          155..1171
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(209..231)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        1569..1948
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    1962..2096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(2689..2757,2758..3549)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2907..2926)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3720..4308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4209..4228
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4462..4479
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    4547..4681
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4687..4882)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4701..4836
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    4941..4962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4977..4982,4983..5000,5001..5007)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5015..5031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5020..5042
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5039..5055
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5039..5055
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5102..5326
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5315..5332
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5413..6012)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5496..5516)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5993..6012
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    join(6013..6021,6022..6024,6025..6599)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6186..6205)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6415..6432
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(6686..7396)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6964..6983)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7233..7251
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(7397..7438)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    7446..7470
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9316..9340)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(9350..9363,1..10))
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccttgtagtc gccgctctgg aagtacaggt tctcaccgct gcccttcgtc ggcttgtagg
       61 cgtccaccat cacgatgtgc gccatggtgc tagcctcgag gaattctttg ccaaaatgat
      121 gagacagcac aataaccagc acgttgccca ggagctgtag gaaaaagaag aaggcatgaa
      181 catggttagc agaggctcta gagccgccgg tcacacgcca gaagccgaac cccgccctgc
      241 cccgtccccc ccgaaggcag ccgtcccccc gcggacagcc ccgaggctgg agagggagaa
      301 ggggacggcg gcgcggcgac gcacgaaggc cctccccgcc catttccttc ctgccggcgc
      361 cgcaccgctt cgccccgcgc ccgctagagg gggtgcggcg gcgcctccca gatttcggct
      421 ccgcacagat ttgggacaaa ggaagtccct gcgccctctc gcacgattac cataaaaggc
      481 aatggctgcg gctcgccgcg cctcgacagc cgccggcgct ccgggggccg ccgcgcccct
      541 cccccgagcc ctccccggcc cgaggcggcc ccgccccgcc cggcaccccc acctgccgcc
      601 accccccgcc cggcacggcg agccccgcgc cacgccccgt acggagcccc gcacccgaag
      661 ccgggccgtg ctcagcaact cggggagggg ggtgcagggg gggttgcagc ccgaccgacg
      721 cgcccacacc ccctgctcac ccccccacgc acacaccccg cacgcagcct ttgttcccct
      781 cgcagccccc cccgcaccgc ggggcaccgc ccccggccgc gctcccctcg cgcacactgc
      841 ggagcgcaca aagccccgcg ccgcgcccgc agcgctcaca gccgccgggc agcgcggagc
      901 cgcacgcggc gctccccacg cacacacaca cgcacgcacc ccccgagccg ctccccccgc
      961 acaaagggcc ctcccggagc ccctcaaggc tttcacgcag ccacagaaaa gaaacaagcc
     1021 gtcattaaac caagcgctaa ttacagcccg gaggagaagg gccgtcccgc ccgctcacct
     1081 gtgggagtaa cgcggtcagt cagagccggg gcgggcggcg cgaggcggcg gcggagcggg
     1141 gcacggggcg aaggcagcgc gcagcgactc ccgcccgccg cgcgcttcgc tttttatagg
     1201 gccgccgccg ccgccgcctc gccataaaag gaaactttcg gagcgcgccg ctctgattgg
     1261 ctgccgccgc acctctccgc ctcgccccgc cccgcccgcc gccccttttt ttttttgggc
     1321 gccccccccc cccccccccc ccaaaaaaaa aaaccttcgg ggcccccccc cccctggcgc
     1381 cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc gccccgcctg
     1441 gcgcgcgccc cccccccccc cgcccccatc gctgcacaaa ataattaaaa aataaataaa
     1501 tacaaaattg ggggtgggga ggggggggag atggggagag tgaagcagaa cgtggggctc
     1561 acctcgacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     1621 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     1681 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     1741 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     1801 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     1861 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     1921 tattaataac tagtcaataa tcaatgtcga cgatgatatc agatccagac atgataagat
     1981 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2041 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaatt
     2101 cgattcagct tcagaagatg gcggagggcc tccaacacag taattttcct cccgactctt
     2161 aaaatagaaa atgtcaagtc agttaagcag gaagtgacta actgaccgca gctggccgtg
     2221 cgacatcctc ttttaattag ttgctaggca actgccctcc agagggcagt gtggttttgc
     2281 aagaggaagc aaaaagcctc tccacccagg cctggaatgt cagtgtggtt ttgcaagagg
     2341 aagcaaaaag cctctccacc caggcctgga atgtttccac ccaatcatta ctatgacaac
     2401 agctgttttt tttagtatta agcagaggcc gggggcccct ggcctccgct tactctggag
     2461 aaaaagaaga gaggcttcca gaggcaactt gtcaaaacag gactggcgcc ttggaggcgc
     2521 tgtggggcca cccaaattga tataattaag ccccaaccgc ctcttcccgc ctcatttcag
     2581 cctcaccact atgtgaatat agctgaatac acagtttatt gaataaacat taatttccag
     2641 gaaatacagt ctttgttttt ccaaagcggt cattgaaaaa ggaagagtat gagtattcaa
     2701 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2761 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2821 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2881 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2941 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3001 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3061 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3121 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3181 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3241 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3301 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     3361 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     3421 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3481 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3541 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3601 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3661 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3721 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3781 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3841 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3901 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3961 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4021 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4081 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4141 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4201 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4261 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4321 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4381 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4441 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4501 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcggatc cagacatgat
     4561 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4621 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4681 tagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     4741 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     4801 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     4861 ggttgctgac taattgagat gcggatccgc tggcacgaca ggtttcccga ctggaaagcg
     4921 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     4981 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     5041 ggaaacagct atgaccatga ttacgccaag cttgggctgc aggttctttc cgcctcagaa
     5101 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     5161 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     5221 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     5281 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     5341 cgagctctag atcatcgatg catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc
     5401 gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg
     5461 cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg
     5521 cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag
     5581 cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag
     5641 gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc
     5701 ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc
     5761 cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca
     5821 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg
     5881 ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc
     5941 ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt
     6001 gtactcggtc atggttgtgg ccatattatc atcgtgtttt tcaaaggaaa accacgtccc
     6061 cgtggttcgg ggggcctaga cgttttttta acctcgacta aacacatgta aagcatgtgc
     6121 accgaggccc cagatcagat cccatacaat ggggtacctt ctgggcatcc ttcagcccct
     6181 tgttgaatac gcttgaggag agccatttga ctctttccac aactatccaa ctcacaacgt
     6241 ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac acgtggcttt tggccgcaga
     6301 ggcacctgtc gccaggtggg gggttccgct gcctgcaaag ggtcgctaca gacgttgttt
     6361 gtcttcaaga agcttccaga ggaactgctt ccttcacgac attcaacaga ccttgcattc
     6421 ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa gaagacaggg ccaggtttcc
     6481 gggccctcac attgccaaaa gacggcaata tggtggaaaa taacatatag acaaacgcac
     6541 accggcctta ttccaagcgg cttcggccag taacgttagg ggggggggag ggagaggggg
     6601 ggggggcgga attcgatatc aagcttatcg agcggcctgt cgacttaacg cgttgattgt
     6661 cgacttaacc ggtactagtt cattactact tgtacagctc gtccatgccg ccggtggagt
     6721 ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga
     6781 tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt
     6841 aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga
     6901 tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca
     6961 tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct
     7021 tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt
     7081 cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt
     7141 cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca
     7201 ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg
     7261 tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg
     7321 agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct
     7381 cgcccttgct caccatcgta gaatcgagac cgaggagagg gttagggata ggcttaccga
     7441 tatcaaccac tttgtacaag aaagttgggt agtagatgta tggaaatata cggtagggca
     7501 cacgctgaca gtacttttcc caagccacgc cgtatttctt cttacagtgg tactcgtcac
     7561 gagcttctcg gtggacaagc aacatggtga aataaattat gtagaaataa ggcagaatgt
     7621 ggttaaaacc acatgggagg gaccacgcca aggccatgat gagatcaccc aagtaattgg
     7681 ggtggcgaac aaagccccac catccagaaa ctagaagatt ttttcccgtt gaagtatgaa
     7741 tggtttttaa atgtgcaagc tttggatcac tgggattttt ccggaatgca tttttctgag
     7801 aatttgcacc tcggaagatt acataaccac aaagtttcag aacaataatt agagaagcca
     7861 ttggccaaga cacttcattt ggatgactga ctaaataaaa ggcttggaag ctgtaaataa
     7921 agggaaccca caccaagtct ccaaaagcca gcatgaatcc aaatccatcg tggatgatgt
     7981 ccatggtcgt caacaacgct tcctcattcc agagagcatc caccacatag agaagctgga
     8041 aactattaac taaaatcatg gccaaggatg gaacagcgcg gtcctgtatt ttcatttcag
     8101 ccaaaagcat caccaagtta ataaccaccc atccaatcaa tccggggcgc aattcacaaa
     8161 agtatttgag atcaaaagta ccaattcgag ggtttaattc acggccaatg aagaaatcat
     8221 agacagcatt tccagagctg gcaggcgaca ggtcattccg gggcgctttc aaagagcgca
     8281 tgtagagata cacactcaag accacacaaa aaacagtggc cgcaagtgca aactgaagaa
     8341 aatgactgta cacgtaatga aactctacgc cctggaagag agatgttccg atgactgcag
     8401 atgtcaggat aaaagcatag aatccattta atctatactt gagtcttctt ccatcaataa
     8461 gaggcgttcc ttctacaacc tttccaattg gcagtaggta gaacaggact tgaatcaaaa
     8521 accacaggag gtagacccca aatactctgg tttcccataa ctcatacaaa gctggcaaag
     8581 gaggagggaa attcagaaga ctgggatctt tctgtttaca catcaacagc aacaggaaga
     8641 ggaacacagg caggccaaac atgatgagaa acacaccagg tactcctcca aactccaagt
     8701 cctttgcccg gatgggggtc acttcaaagg ttctcactgc cagttctttt gcaacgtatt
     8761 tttcttcctt agaatctatt tcttttaatt tgacttcttc tcttcttgga cgaaggctat
     8821 actgtgttgc tatgtaactg ctttcttgtg acaaactgaa tttttcctgt gtatttttat
     8881 gaggtgcgtc atttctctca atatgctcag gctccccatt atatctgctg atgctatttc
     8941 caaatggctt cagaatcagc ggagtcaatt taacttccac ttccctcctt gcttccttaa
     9001 tgtcggcctg gtgggaagca gaagcagatc ggcgggcact tttaggtggt cgaccagggg
     9061 atcgggagcg tgaccttgat cgactccctc ggcgtctgga aggggaactg gaagttgagc
     9121 cacctttcct ttgcctaaag gaagttaaag gcttaatatc attctctttc aattcaagct
     9181 ctgttccatc tttatacttc acagtgtaaa gctgggaggt gctgtcgtgg ctcagaattt
     9241 ctacttcata ataaagtgaa ctcccaggcc atcgacctct taccacttca ccatcggcaa
     9301 atttcctact tggcatgcca acttttttgt acaaacttgt tggcgcgccc ttgtcatcgt
     9361 cat
//
