LOCUS       sequence-471951-       15851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express gRNA from Foxtail Mosaic Virus Vector targeting GFP 
            (T-DNA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15851)
  AUTHORS   Baysal C, Kausch AP, Cody JP, Altpeter F, Voytas DF
  TITLE     Rapid and efficient in planta genome editing in sorghum using 
            foxtail mosaic virus-mediated sgRNA delivery.
  JOURNAL   Plant J. 2025 Jan;121(2):e17196. doi: 10.1111/tpj.17196. Epub 2024 
            Dec 11.
  PUBMED    39661735
REFERENCE   2  (bases 1 to 15851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2025-01"; volume: "121(2):e17196. doi"; pages: " 
            10.1111/tpj.17196. Epub 2024 Dec 11"
COMMENT     Sequence Label: pCB036 FoMV-sgGFP
FEATURES             Location/Qualifiers
     source          1..15851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      66..318
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    336..360
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1661..2290
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2719..3792
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3858..4052
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4288..4310)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4396..4536
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4451..4470
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4551..4568)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4722..5310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4802..4821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5397..6191)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6616..6640
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6718..6892
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6949..7746)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7060..7079)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7670..7689
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7809..8485)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8676..8697
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8712..8717,8718..8735,8736..8742)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    8750..8766
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8755..8777
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8774..8790
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8774..8790
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9155..9500
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      10097..10106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaaaaaaaaa aaaaaaaacc tggatcctag gttcacaaag tgtcatcgat agctcgaatt
       61 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
      121 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
      181 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
      241 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
      301 catctatgtt actagatcgg gaaactatca gtgtttgaca ggatatattg gcgggtaaac
      361 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
      421 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
      481 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
      541 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
      601 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
      661 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
      721 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
      781 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
      841 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
      901 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
      961 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     1021 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     1081 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     1141 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     1201 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     1261 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     1321 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     1381 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     1441 tgttcgagcc gcccgcgcac ggctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     1501 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     1561 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     1621 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     1681 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     1741 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     1801 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     1861 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     1921 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     1981 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     2041 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     2101 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     2161 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     2221 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     2281 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     2341 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     2401 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     2461 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     2521 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     2581 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     2641 cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc
     2701 caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg
     2761 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     2821 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     2881 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     2941 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     3001 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     3061 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     3121 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     3181 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     3241 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     3301 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     3361 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     3421 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     3481 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     3541 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     3601 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     3661 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     3721 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     3781 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     3841 cgaaaaggcc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     3901 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     3961 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     4021 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agcccaagag
     4081 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     4141 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     4201 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     4261 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg aaacggtcac
     4321 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4381 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     4441 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     4501 ccgcacagat gcgtaaggag aaaataccgc atcaggccct cttccgcttc ctcgctcact
     4561 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4621 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4681 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4741 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4801 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4861 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4921 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4981 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5041 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5101 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5161 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5221 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5281 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5341 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtactaa
     5401 aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt
     5461 aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg
     5521 cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca
     5581 cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca
     5641 agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat
     5701 ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa
     5761 tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg
     5821 gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca
     5881 tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag
     5941 ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc
     6001 atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt
     6061 tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct
     6121 tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc
     6181 attttagcca tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag
     6241 ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc
     6301 agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc
     6361 cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     6421 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     6481 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
     6541 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
     6601 ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact
     6661 taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcgggggat
     6721 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt
     6781 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat
     6841 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aactcgagct
     6901 tgtcgatcga ctctagctag aggatcgatc cgaaccccag agtcccgctc agaagaactc
     6961 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     7021 gaggaagcgg tcagcccatt cgccgccaag ttcttcagca atatcacggg tagccaacgc
     7081 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     7141 gccattttcc accatgatat tcggcaagca ggcatcgcca tgtgtcacga cgagatcctc
     7201 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     7261 ttcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     7321 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     7381 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     7441 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     7501 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     7561 ctggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     7621 cgctgacagc cgaaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     7681 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     7741 ccccatggtc gatcgacaga tctgcgaaag ctcgagagag atagatttgt agagagagac
     7801 tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct
     7861 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc
     7921 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
     7981 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
     8041 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
     8101 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
     8161 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
     8221 tgctccacca tgttcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
     8281 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
     8341 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
     8401 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
     8461 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
     8521 attcttggag tagacgagag tgtcgtgctc caccatgttg gcaagctgct ctagccaata
     8581 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8641 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8701 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggaat tgtgagcgga
     8761 taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc ctccaaaaat
     8821 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
     8881 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
     8941 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
     9001 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     9061 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatac tccaaaaata
     9121 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat
     9181 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
     9241 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
     9301 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     9361 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     9421 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     9481 catttcattt ggagagggaa aactcttccg aaaccgaaac tgactgaaac tacctcgacc
     9541 gaccttagaa cccaagaacc caacgggtgc ggccactatg tccatcgagg cagttttcga
     9601 ccaggttacc gacccatcgc tccgcgctgt gattcaggag gaagcgcaca aacagatcaa
     9661 agatttattt aaggaaacga cgcgctgcaa tccctactcc ataccgcaag ctgggcgcaa
     9721 ggttttggaa aagtacgcca tcccctacaa cccgtactct ctcaaactac accctcacgc
     9781 agcctcaaaa gcgtttgaag tgtcgctcta cgaggctgcg tctaactacc tcccctccac
     9841 ctcctcaact cctgtcacat tcatgttcac aaaaccgggc aagctcagat tctttaggcg
     9901 ccgaggtcat gtggacaaat tcgttaatgc tgacatagtt ccaagagact tggctagata
     9961 cccacgcgac acagtctaca gttatctgcc cgagatcacc accacacacg ctttcattgg
    10021 cgacaccctg catcacttcg gtgaggactt tctcgtcgag gttttctcca ggtcaccgaa
    10081 actagaagtg cttctagcca ccatggtatt accacccgaa gccttttaca gaatggagtc
    10141 cctccatccc tcggtttaca ctctcctcta cagggacgac cgattcctat acctgcctgg
    10201 tggcctatct ggcggtgagt acgaacatcg ctataaggac ctaaactggc taacatttgg
    10261 cacggttacg cacggcggga tcactatcac aggagaacgc attgagacta aggccgcgaa
    10321 tcatcttttc ctcttcagac gagggcgact agcgacacca aaattccgct cattcgatat
    10381 gcccgagcct atggtcctgc ttcccaaggt tttccgcccc gcaaagtaca atgtacagaa
    10441 gccaattccc cgggagaaag caaacaaatg gttgatgtac gttaaatcca tcggcaatgc
    10501 caccattcgt gacgtatggg ctaagctgag gcaaaccata gccaatgcag acattggact
    10561 cttctcgccc actgagctcg tgcatctcac gaattacttc ctgctcctgg gccggcttga
    10621 ctcacacaac tccttcgacc aagtactggc cgacagtgtg ctgaaagcat ggttcagacc
    10681 aatggtcgca aagcttcagg agatcaagca caaactcatg gggcagaccc aattcatgca
    10741 actctgccaa gcgctagaga tgacggaggt ggacctcgtc tttgaggtcc gggactccaa
    10801 gaccccccac aaacaagctg tgccattgga ccgtgaaatt gaaaacgttt tgttggaagg
    10861 agtctcatcg gagccaactt acactgaaac cgaaggcgtt gctgatggtc cacttccccc
    10921 cccaatgcaa actgcagccg agccgtccgc gacctcagac gagcctgaga gctctagctc
    10981 gcgtgaaatt gagcaccaac cggcgcctga gatcacgctt gacgaggaag aacctcagcg
    11041 agacgatctg ccttgggacg cttggagaac acaattaagg gcgcttggct tcgaggcctc
    11101 agaaaggcag tatgacccgg acggtgaact gatctctcct atcctgagca cccgaaggtt
    11161 gcctaaaact cccataaaca caacactctg cgccacgcta gacaagattg cacgctgccc
    11221 aactttctac aagcctgaca cagatcgcgc gcagacttac gctcgcgacg tcatggcggg
    11281 gaaaaccggt gctattctca agcaacaacc ctttgagtgg aaaaccacgc tcaagcgcaa
    11341 gactaaagag gaaccgaagg aaattcacct tgcggtgttg catggtgcgg gcgggtcggg
    11401 caaatcctac gcactgcagg aatttatgcg gaacaaccct gacacaccaa ttacggtcat
    11461 cctgccgact aacgagctca gggccgactg gaagaaaaaa ttgcccgccc acgacaaaga
    11521 cacatttatg acatacgaaa acgcgctctt gtgccctcgt ggagacatct tcattatgga
    11581 cgattacaca aaattgccca ggggctacat tgaggctttc gtgcagaatg cacctgccct
    11641 ctcacttctg atactcaccg gtgaccccaa ccaagccgaa cactttgaga ccactgagga
    11701 caatgaaatt aacagcctcg cccccgcctc agtggtcttc ggcaagttct ctaggtacca
    11761 cataaatgcc acacaccgca accccagaaa cttggcaaac gccctcggtg tttactccga
    11821 gacgcccggg gaggttaaag tgctttacac gaggaacatc aagaccggtt atcacaatct
    11881 cgtgccctca caaatgaaga tgagaaatta cgcctcactc gggcagcgag cgtccaccta
    11941 tgcgggttgt caggggatca ccgcgccccg cgttcaaatc atcctagact ccgacacacc
    12001 ccggtgcacc aggcaagtca tgtacactgc actctcaagg gccacgacgg aagtggtgct
    12061 ctgcaacacg atgccggatg agaaaagctt tttccagaag gttgaagcaa caccgtacct
    12121 caaagccatc ctcaacctca acaaagagat taaagtcact gagggtgact tgacagaaga
    12181 accgccgagg gagcccgctc ctcccaccac acacctacct gttgaaaaca gaattattct
    12241 taatgaggcc ctagtcgaac cgctgcccga caaacatgac cgcgagatct actccaactc
    12301 cactggcttt tcaaactgca tacagactca agacccgtac atccaagcct tccaacatca
    12361 gcaggccaag gacgagacat tgttctgggc aaccgtcgag aagaggctcg cagcatctac
    12421 gccgaaggac aactggacag aattcaagac caagagacct ctgggtgacg tgctttggct
    12481 cgcgtacaag cgggcgatgg agctcccaga tgagcccatc aaatttaacc cagagctctg
    12541 gtgggcatgt gcagacgagg tgcaaaagac ctacctctcc aagcccatac acgcactcaa
    12601 gaacggaatc cttcggcaat cacccgactt tgactggaac aaactgcaga ttttcctcaa
    12661 gtcacagtgg gttaagaaga ttgacaaaat cgggaaaatt gacgtcaacg ctggacagac
    12721 aattgccgcc ttttaccaac caaccgttat gctgtttgga accatggcga gatacatgcg
    12781 ccgcatccgc gacacttatc aaccccgcga aatactcatc aattgcgaga agaaccagaa
    12841 gcacatttcg aagtgggtcg agagcaattg gaaccaccgc ctacctgctt acaccaatga
    12901 cttcaccgct tacgaccaaa gccaagacgg ggctatgtta cagtttgagg tgctgaaagc
    12961 cctgcaccat gatatccctc atgaggttgt ggaagcctac gtagccctta aactcaactc
    13021 aaaaatgttt ctgggcacac tggcgattat gagattaact ggtgagggac ccaccttcga
    13081 cgctaacaca gagtgcaaca ttgcttacac acacgcccgg ttcgagatcc caaagaatgt
    13141 ggcgcaaatg tacgcgggtg acgactgtgc gctcaactgc aggcccgttg aacggcagtc
    13201 cttcctgcct cttgtggaga aattcaccct gaaatcaaaa cctaaagtat ttgagcaaaa
    13261 agttgggtca tggcctgagt tctgcggcaa tctgatcacc ccacggggct acctcaagga
    13321 tcccatgaag ctacaacact gcctgcaact ggcacagagg aagaaaccat ccgaacctgg
    13381 gtcgcttaaa gacgttgctg agaactacgc tatggactta ctacccacat acgagctagg
    13441 ggatgcactc tacgaaatct tcgacgagag acaaatgaac gcgcactatc agtcggtcag
    13501 gacgcttatc acatgcgccc acaccaaagt cctccgagtt gcacaggcac ttcaggaaga
    13561 ctgcaccttc tttagctcca tctaacaggt tttgagttag gctaactcca ctgacgaatt
    13621 gaataacaat ggatagtgaa atagttgaac gactaacaaa gcttggtttc gtcaagactt
    13681 cacacacgca catcgctggc gagcccctcg tgattcacgc cgttgctggg gccggtaaaa
    13741 ccaccctcct tcggtcctta cttgaattac ctggagtgga agtcttcaca ggcggggagc
    13801 acgatcctcc aaatttgtca gggaaatata tccgctgcgc tgcaccccct gtggccggtg
    13861 catacaacat tcttgacgag taccccgcgt acccaaattg gcgatcgcaa ccctggaacg
    13921 tcctaatcgc cgacaaccta caatacaaag aacccacagc tcgcgcccac tacacatgca
    13981 atcgcactca ccgcctgggg cagctcactg tcgacgcttt gcgtagggtt ggtttcgaca
    14041 tcacctttgc cggcacgcag actgaagact acggattcca ggaaggccat ctctacacta
    14101 gtcaattcta cggacaggtc atttcacttg acacgcaggc ccataagatc gctgtgcgcc
    14161 acggactcgc acccctgtcc gctttagaaa cccgggggct ggaatttgat gagaccactg
    14221 tgataacgac taaaacctcg ctggaggaag tgaaggacag gcacatggtc tatgtcgctc
    14281 tcacaaggca caggcgcacc tgccatctct acaccgctca ctttgcgccc tccgcctgac
    14341 aacacgaaag ccatactaac tgtagctata ggaatagccg cctccctagt ctttttcatg
    14401 ctcacacgca acaatctgcc acacgtcggt gataacatcc actcactacc ccacggagga
    14461 agttatattg acggtaccaa gtccatcaac taccgcccac ctgcgtcacg ctacccctca
    14521 tccaacttac tcgctttcgc tccaccaata ctcgccgcag tactcttttt cctcacacag
    14581 ccatatctag ctaccagacg atccaggtgc gttcggtgct tcgttgtcca cggggcatgc
    14641 acgaatcaca cctagttgtt atattagcgc tgttacttat agctctgtgg tgtctaagca
    14701 ctcgacccgt tcaaccatcg tgccacgtcg aaatcaacgg ccactccatc atcgtcaccg
    14761 gaaactgctg gcactccaca cagcgtcccc actgagggtg ttagggtaac caacatcagt
    14821 gaagagaaac ctggtcacca ccctgaccta gtttaagagc tatgctggaa acagcatagc
    14881 aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcgccc
    14941 cactcaacga ccgcattgag ggtgttaggg taaccaacat cagtgaagag aaacaaccca
    15001 cctcaagtgt gacctcatca tttcaggaca caatggcaac acaaaatgcc gacgtcactg
    15061 atgcgacgga ctacaagaaa ccgcctgctg aaactgagca gaaggcactc accattcaac
    15121 cacggtcaaa caaggcgccc agtgacgagg agttggtacg catcatcaac gcggcgcaga
    15181 agcgaggcct cacacccgcg gcctttgttc aagcagctat agtcttcacc atggaatcca
    15241 tggacaaggg cgccaccgac tccacgattt tcacgggaaa atacaacact ttcccaatga
    15301 aaagtctggc gctagcttgc aaagatgctg gcgtgcccgt gcacaaactt tgctacttct
    15361 ataccaagcc ggcttacgcg aaccgtaggg tcgccaacca gccgcctgct cgctggacta
    15421 acgagaatgt gcccaaagct aacaagtggg cggctttcga caccttcgac gcacttctcg
    15481 acccatacgt agtcccatcc tctgtaccgt acgatgagcc cacgccagag gatcgccaag
    15541 tcaatgagat tttcaagaag gacaatttga gtcaggcagc atccagaaac caactcctag
    15601 gaacgcaagc ctccatcacg cgcgggagac tcaacggcgc accagcacta ccaaacaacg
    15661 ggcagtactt catcgaggca cctcagtaat ctagaagatg ggactcgagc gatcagtagt
    15721 atgataccaa taaataaatc gggcgaatcc gcgcctcctg actatgggca ggtttacgga
    15781 ccaagctgta tcgagatacg acctaacagt aacgcagcta aggggtgaat gcacacatcg
    15841 cttataaaaa a
//
