LOCUS       sequence-471955-       13583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express flourescent protein AmCyan from the gamma genome of 
            Barley Stripe Mosaic Virus Vector (T-DNA) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Baysal C, Kausch AP, Cody JP, Altpeter F, Voytas DF
  TITLE     Rapid and efficient in planta genome editing in sorghum using 
            foxtail mosaic virus-mediated sgRNA delivery.
  JOURNAL   Plant J. 2025 Jan;121(2):e17196. doi: 10.1111/tpj.17196. Epub 2024 
            Dec 11.
  PUBMED    39661735
REFERENCE   2  (bases 1 to 13583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2025-01"; volume: "121(2):e17196. doi"; pages: " 
            10.1111/tpj.17196. Epub 2024 Dec 11"
COMMENT     Sequence Label: pGY037 BSMV-gamma-AmCyan
FEATURES             Location/Qualifiers
     source          1..13583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    103..277
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(318..1343)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(1410..2086)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    2277..2298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2313..2318,2319..2336,2337..2343)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2351..2367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2356..2378
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2375..2391
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2375..2391
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3141..3186
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             5731..6420
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan1
                     /note="mammalian codon-optimized"
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPLAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKMTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHAVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPF"
     misc_RNA        6421..6496
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     polyA_signal    6900..7076
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7085..7102)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7085..7101)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7094..7116)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7304..7328
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8629..9258
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9687..10760
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10826..11020
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11256..11278)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11364..11504
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11419..11438
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11519..11536)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11690..12278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11770..11789)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12365..13159)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat
      301 cccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca
      361 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
      421 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
      481 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
      541 cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag
      601 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
      661 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
      721 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
      781 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
      841 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
      901 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
      961 ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc
     1021 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg aacggcggga gatgcaatag
     1081 gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat
     1141 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
     1201 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
     1261 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
     1321 gcggtgagtt caggcttttt catatctcat tgccccccgg atctgcgaaa gctcgagaga
     1381 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
     1441 ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
     1501 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     1561 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
     1621 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
     1681 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
     1741 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
     1801 ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga
     1861 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     1921 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     1981 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     2041 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     2101 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     2161 ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2221 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     2281 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     2341 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
     2401 aattctgaag cttgcatgcc tgcaggtcaa catggtggag cacgacactc tcgtctactc
     2461 caagaatatc aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag
     2521 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag
     2581 gacagtagaa aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat
     2641 cgttcaagat gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat
     2701 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatgccgc
     2761 ctgcaggtca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     2821 gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     2881 ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat
     2941 ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc
     3001 gacagtggtc ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt
     3061 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     3121 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     3181 gagaggtata gcttgagcat taccgtcgtg taattgcaac acttggcttg ccaaataacg
     3241 ctaaagcgtt cacgaaacaa acaacacttc ggcatggatg ttgtgaagaa attcgccgtc
     3301 atgtcagtga ctgtagtagc aggtcccgtc cttacgcttt catcacctgt ggtggtgacg
     3361 tttggaacag gcttaattgc cgtatctttg gtgaaacggt tgctacagga acaaccccgt
     3421 gtaattgctc acgatcacga acattaccca ggtggttctg agagcagttc tagctcttgt
     3481 gctaccgcgc ctattttacg taatctttcg cgagatcagt gcgattcaga gaatattgga
     3541 tgcagttcta gcgcctgttc tccgtctgaa attgtgaaag ttacaaggca ggtagtggga
     3601 gttgaacgtg gtctttaccg ggacattttt caggacaacg aaatcccatc agtcatggaa
     3661 gagaaactgc agaaactcct ttactctgag ggtgagaaga ttcgaagacg ttgccaattt
     3721 gaagcatcaa cgatgcactc acgcaaagta aaggttccgg aggtaggtac tatcccagat
     3781 atccaaactt ggttcgatgc tacgtttcct ggtaactccg ttaggttttc tgatttcgac
     3841 ggttatactg ttgctacgga ggacattaac atggatgttc aggattgtag acttaagttc
     3901 gggaagactt ttcgacctta tgaatttaag gaatcactga aaccagtact gaggacagca
     3961 atgccagaaa aacgacaggg tagtttgatt gaaagtgtgc tggcctttcg taaaagaaat
     4021 ttggctgcgc ccagattaca aggagctttg aatgaatggc acacaattga gaatgtgcta
     4081 acgaaggcgt taaaggtatt cttctttgaa gatttaattg atcgaacgga tcactgcact
     4141 tacgagtcag cgctcagatg gtgggataaa caatcagtga cagctcgagc gcagctcgtg
     4201 gcggatcagc ggaggttatg tgatgttgac ttcacgactt ataacttcat gataaaaaat
     4261 gatgtaaagc cgaagttaga tctaacacct caagttgaat atgcagcttt gcagactgtt
     4321 gtatatcctg ataagatagt caatgctttc tttggtccga tcataaagga gattaatgaa
     4381 cggatcatca gagcgcttag acctcatgtg gtctttaatt ctcgtatgac tgctgatgaa
     4441 ctgaatgaaa cagctgcctt tttgacacct cataagtaca gagccttaga gattgatttt
     4501 tcaaaatttg ataaatcaaa gactgggctt catatcaaag ctgtcattgg actctataag
     4561 ctctttggcc tagatggcct gttaaaagtg ctctgggaaa aatcgcaata tcagacttac
     4621 gtgaaagata gaaacttcgg tctcgaggca tatctattgt atcagcaaaa gtcaggaaat
     4681 tgtgacactt acggttcgaa cacctggtct gccgccttgg cgttgttaga ttgtcttcct
     4741 ttggaagatg cacatttctg tgtatttggt ggtgatgatt cattgatatt gtttgatcag
     4801 ggatacataa tttccgaccc atgccggcaa cttgccggta cttggaatct tgaatgtaaa
     4861 gtgttcgact tcaagtaccc cgcattttgt ggtaaatttc tgctgtgcat agatggaaaa
     4921 tatcaatttg ttccagatgc ggcaaaattt atcacaaaat taggtagaac tgatgtgaga
     4981 gatgtagaag ttttgagtga gatttatatc tctatcaatg acaattacaa atcttacaaa
     5041 gactttaagg tgcttgatgc tttggataag gctttagtgg atagatatcg atccccttat
     5101 agtgctattt ctgctttggt ttctttatgt tatcatatct ttgactttaa taagtttaag
     5161 ttgctgttta attgtgaagg gaaatttgtg gataagaagc tgagaaaaga cttcgagtgg
     5221 tgaactctag gtcctgatgt ttaaatctac tgtatttacc ttcgcatgat ggctactttc
     5281 tcttgtgtgt gttgtggtac cttaactaca agtacttact gtggtaagag atgtgagcga
     5341 aagcatgtat attctgaaac aagaaataag agattggaac tttacaagaa gtatctattg
     5401 gaaccgcaaa aatgcgccct gaatggaatc gttggacaca gttgtggaat gccatgctcc
     5461 attgcggaag aggcttgtga tcaactgcca atcgtgagta ggttctgtgg ccaaaagcat
     5521 gcggatctgt atgattcact tctgaaacgt tctgaacagg agttacttct tgaatttctc
     5581 cagaagaaga tgcaggagct gaaactttct catatcgtaa aaatggctaa gcttgaaagt
     5641 gaggttaacg caatacgtaa gtccgtagct tcttcttttg aagattctgt tggatgtgat
     5701 gattcttctt ccgtttctaa gtaactattg atggccctgt ccaacaagtt catcggcgac
     5761 gacatgaaga tgacctacca catggacggc tgcgtgaacg gccactactt caccgtgaag
     5821 ggcgagggca gcggcaagcc ctacgagggc acccagacct ccaccttcaa ggtgaccatg
     5881 gccaacggcg gccccctggc cttctccttc gacatcctgt ccaccgtgtt catgtacggc
     5941 aaccgctgct tcaccgccta ccccaccagc atgcccgact acttcaagca ggccttcccc
     6001 gacggcatgt cctacgagag aaccttcacc tacgaggacg gcggcgtggc caccgccagc
     6061 tgggagatca gcctgaaggg caactgcttc gagcacaagt ccaccttcca cggcgtgaac
     6121 ttccccgccg acggccccgt gatggccaag atgaccaccg gctgggaccc ctccttcgag
     6181 aagatgaccg tgtgcgacgg catcttgaag ggcgacgtga ccgccttcct gatgctgcag
     6241 ggcggcggca actacagatg ccagttccac acctcctaca agaccaagaa gcccgtgacc
     6301 atgcccccca accacgcggt ggagcaccgc atcgccagaa ccgacctgga caagggcggc
     6361 aacagcgtgc agctcaccga gcacgccgtg gcccacatca cctccgtggt gcccttctag
     6421 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     6481 ggcaccgagt cggtgctttt tttaaaaaaa aaaaaatgtt tgatcagatc attcaaatct
     6541 gatggtgccc atcaaccata tgatgggagt gtttgcaagt ccactataat cgaacttgaa
     6601 aacgatgcct gaattggaaa ccatgaatct taacggactc tggagagaaa atttaggaat
     6661 tggtatgtaa gctacaactt ccggtagctg cgtcacactt taagagtgtg catactgagc
     6721 cgaagctcag cttcggtccc ccaagggaag accacccggg tcggcatggc atctccacct
     6781 cctcgcggtc cgacctgggc atccgaagga ggacgtcgtc cactcggatg gctaagggag
     6841 agctcgaatt cggtacgctg aaatcaccag tctctctcta caaatctatc tctctctatt
     6901 ttctccataa ataatgtgtg agtagtttcc cgataaggga aattagggtt cttatagggt
     6961 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     7021 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatcagct
     7081 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7141 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7201 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
     7261 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
     7321 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     7381 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     7441 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     7501 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     7561 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     7621 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     7681 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     7741 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     7801 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     7861 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     7921 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     7981 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     8041 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     8101 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     8161 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     8221 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     8281 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     8341 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     8401 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc
     8461 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     8521 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     8581 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     8641 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     8701 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     8761 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     8821 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     8881 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     8941 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     9001 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     9061 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     9121 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     9181 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     9241 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     9301 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     9361 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     9421 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     9481 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     9541 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     9601 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     9661 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     9721 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     9781 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     9841 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     9901 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     9961 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
    10021 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
    10081 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
    10141 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
    10201 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
    10261 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
    10321 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
    10381 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
    10441 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
    10501 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
    10561 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
    10621 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
    10681 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
    10741 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
    10801 aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
    10861 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
    10921 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
    10981 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
    11041 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
    11101 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
    11161 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
    11221 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
    11281 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    11341 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
    11401 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    11461 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
    11521 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    11581 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    11641 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    11701 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    11761 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    11821 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    11881 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    11941 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    12001 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    12061 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    12121 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    12181 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    12241 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    12301 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
    12361 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
    12421 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
    12481 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
    12541 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
    12601 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
    12661 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
    12721 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
    12781 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
    12841 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
    12901 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
    12961 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
    13021 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
    13081 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    13141 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
    13201 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    13261 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    13321 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
    13381 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
    13441 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
    13501 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
    13561 tcggggagct gttggctggc tgg
//
