LOCUS       sequence-471959-       12561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible lentiviral transfer vector with PTP-PEST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12561)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 12561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pINDUCER20-PTPN12
FEATURES             Location/Qualifiers
     source          1..12561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5148..5172)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5225..5251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5337..6548
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6385..6404
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6607..7224,7225..7230,7231..7266,7267..7269,7270..7305,
                     7306..7308,7309..7344,7345..7353)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7391..7964
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7558..7575)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7785..7804
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7980..8774
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7980..8774
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8034..8053)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8644..8663
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8951..9539
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9004..9024)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9422..9433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9614..9847
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9966..9984)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10052..10156
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10157..10225,10226..11017)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10375..10394)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11188..11776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11677..11696
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11930..11947
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12187..12208
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12223..12228,12229..12246,12247..12253)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12261..12277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12266..12288
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12285..12301
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12285..12301
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12351..12370
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctggcgc cggaaccaat tcagtcgact ggatccggta
     2761 ccgaggagat ctgccgccgc gatcgccatg gagcaagtgg agatcctgag gaaattcatc
     2821 cagagggtcc aggccatgaa gagtcctgac cacaatgggg aggacaactt cgcccgggac
     2881 ttcatgcggt taagaagatt gtctaccaaa tatagaacag aaaagatata tcccacagcc
     2941 actggagaaa aagaagaaaa tgttaaaaag aacagataca aggacatact gccatttgat
     3001 cacagccgag ttaaattgac attaaagact ccttcacaag attcagacta tatcaatgca
     3061 aattttataa agggcgtcta tgggccaaaa gcatatgtag caactcaagg acctttagca
     3121 aatacagtaa tagatttttg gaggatgata tgggagtata atgttgtgat cattgtaatg
     3181 gcctgccgag aatttgagat gggaaggaaa aaatgtgagc gctattggcc tttgtatgga
     3241 gaagacccca taacgtttgc accatttaaa atttcttgtg aggatgaaca agcaagaaca
     3301 gactacttca tcaggacact cttacttgaa tttcaaaatg aatctcgtag gctgtatcag
     3361 tttcattatg tgaactggcc agaccatgat gttccttcat catttgattc tattctggac
     3421 atgataagct taatgaggaa atatcaagaa catgaagatg ttcctatttg tattcattgc
     3481 agtgcaggct gtggaagaac aggtgccatt tgtgccatag attatacgtg gaatttacta
     3541 aaagctggga aaataccaga ggaatttaat gtatttaatt taatacaaga aatgagaaca
     3601 caaaggcatt ctgcagtaca aacaaaggag caatatgaac ttgttcatag agctattgcc
     3661 caactgtttg aaaaacagct acaactatat gaaattcatg gagctcagaa aattgctgat
     3721 ggagtgaatg aaattaacac tgaaaacatg gtcagctcca tagagcctga aaaacaagat
     3781 tctcctcctc caaaaccacc aaggacccgc agttgccttg ttgaagggga tgctaaagaa
     3841 gaaatactgc agccaccgga acctcatcca gtgccaccca tcttgacacc ttctccccct
     3901 tcagcttttc caacagtcac tactgtgtgg caggacaatg atagatacca tccaaagcca
     3961 gtgttgcata tggtttcatc agaacaacat tcagcagacc tcaacagaaa ctatagtaaa
     4021 tcaacagaac ttccagggaa aaatgaatca acaattgaac agatagataa aaaattggaa
     4081 cgaaatttaa gttttgagat taagaaggtc cctctccaag agggaccaaa aagttttgat
     4141 gggaacacac ttttgaatag gggacatgca attaaaatta aatctgcttc accttgtata
     4201 gctgataaaa tctctaagcc acaggaatta agttcagatc taaatgtcgg tgatacttcc
     4261 cagaattctt gtgtggactg cagtgtaaca caatcaaaca aagtttcagt tactccacca
     4321 gaagaatccc agaattcaga cacacctcca aggccagacc gcttgcctct tgatgagaaa
     4381 ggacatgtaa cgtggtcatt tcatggacct gaaaatgcca tacccatacc tgatttatct
     4441 gaaggcaatt cctcagatat caactatcaa actaggaaaa ctgtgagttt aacaccaagt
     4501 cctacaacac aagttgaaac acctgatctt gtggatcatg ataacacttc accactcttc
     4561 agaacacccc tcagttttac taatccactt cactctgatg actcagactc agatgaaaga
     4621 aactctgatg gtgctgtgac ccagaataaa actaatattt caacagcaag tgccacagtt
     4681 tctgctgcca ctagtactga aagcatttct actaggaaag tattgccaat gtccattgct
     4741 agacataata tagcaggaac aacacattca ggtgctgaaa aagatgttga tgttagtgaa
     4801 gattcacctc ctcccctacc tgaaagaact cctgaatcgt ttgtgttagc aagtgaacat
     4861 aatacacctg taagatcgga atggagtgaa cttcaaagtc aggaacgatc tgaacaaaaa
     4921 aagtctgaag gcttgataac ctctgaaaat gagaaatgtg atcatccagc gggaggtatt
     4981 cactatgaaa tgtgcataga atgtccacct actttcagtg acaagagaga acaaatatca
     5041 gaaaatccaa cagaagccac agatattggt tttggtaatc gatgtggaaa acccaaagga
     5101 ccaagagatc caccttcatg cggccgctga taactcgaga tatctagacc cagctttctt
     5161 gtacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
     5221 cgaataccca tacgacgtcc cagactacgc ttagtaatga ttaattaaac tagaaattct
     5281 accgggtagg ggaggcgctt ttcccaaggc agtctggagt aacccaccca agatctggcc
     5341 tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac
     5401 gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc
     5461 ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg
     5521 ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa
     5581 gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt
     5641 atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg
     5701 gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc
     5761 ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag
     5821 ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc
     5881 aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc
     5941 gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct
     6001 aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg
     6061 tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg
     6121 gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga
     6181 cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt
     6241 aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac
     6301 aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt
     6361 tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg
     6421 cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat
     6481 gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt
     6541 tgttagacga agcttggtac cgagctcgga tctccacccc gtaccggtcc tgcagtcgaa
     6601 ttcaccatgt ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat
     6661 ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag
     6721 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag
     6781 atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt
     6841 ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa
     6901 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg
     6961 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc
     7021 cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga
     7081 gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac
     7141 cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag
     7201 aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac
     7261 atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt
     7321 gacgattttg accttgacat gctccccggg taactaagta aggatccgcg gccgcactag
     7381 aggaattccg cccctctccc cccccccccc ctaacgttac tggccgaagc cgcttggaat
     7441 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     7501 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     7561 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     7621 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     7681 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     7741 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     7801 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     7861 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     7921 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttac cggtccacca
     7981 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     8041 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     8101 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     8161 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     8221 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     8281 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     8341 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     8401 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     8461 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     8521 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     8581 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     8641 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     8701 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     8761 acgagttctt ctgatgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc
     8821 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     8881 cataaaatga atgcaattgt tgttgtttag tccctcccaa ttcgatatca agcttatcgc
     8941 gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     9001 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     9061 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     9121 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     9181 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     9241 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     9301 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     9361 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     9421 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     9481 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     9541 tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     9601 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     9661 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9721 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9781 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9841 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     9901 atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg
     9961 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
    10021 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    10081 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    10141 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
    10201 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
    10261 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
    10321 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
    10381 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
    10441 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
    10501 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
    10561 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
    10621 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
    10681 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
    10741 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
    10801 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
    10861 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    10921 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    10981 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    11041 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    11101 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    11161 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    11221 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    11281 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    11341 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    11401 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    11461 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    11521 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    11581 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    11641 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    11701 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    11761 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    11821 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    11881 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    11941 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    12001 tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc
    12061 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    12121 tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    12181 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    12241 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    12301 catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    12361 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    12421 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
    12481 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    12541 atgtactaca aacttagtag t
//
