LOCUS       sequence-471962-       11818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible lentiviral transfer vector with Src 
            constitutively-active mutant (E378G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 11818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pINDUCER20-Src(E378G)
FEATURES             Location/Qualifiers
     source          1..11818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2399,2400..2418,2419..2441,
                     2442..2460,2461..2483,2484..2502)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2535..2573
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2570..2594
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2703..2727
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2765..2774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2771..2812
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     protein_bind    complement(4390..4414)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4467..4493
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4579..5790
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5627..5646
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5849..6466,6467..6472,6473..6508,6509..6511,6512..6547,
                     6548..6550,6551..6586,6587..6595)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6648..7221
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6815..6832)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7042..7061
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7237..8031
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7237..8031
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7291..7310)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7901..7920
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8208..8796
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8261..8281)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8679..8690)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8871..9104
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9223..9241)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9309..9413
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9414..9482,9483..10274)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9632..9651)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10445..11033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10934..10953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11187..11204
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11444..11465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11480..11485,11486..11503,11504..11510)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11518..11534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11523..11545
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11542..11558
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11542..11558
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11608..11627
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt gtttaccact
     2401 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2461 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
     2521 ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     2581 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     2641 gggaccgatc cagcctccgc ggccccgaac tagtccagtg tggtggaatt ctgcagatat
     2701 caacaagttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga ctggatccgg
     2761 taccgccacc atggggagca gcaagagcaa gcccaaggac cccagccagc gccggcgcag
     2821 cctggagcca cccgacagca cccaccacgg gggattccca gcctcgcaga cccccaacaa
     2881 gacagcagcc cccgacacgc accgcacccc cagccgctcc tttggggccg tggccaccga
     2941 gcccaagctc ttcgggggct tcaacacttc tgacaccgtc acgtcgccgc agcgtgccgg
     3001 ggcactggct ggcggcgtca ccactttcgt ggctctctat gactacgagt cccggactga
     3061 aacggacttg tccttcaaga aaggagaacg cctgcagatt gtcaacaaca cggaaggtga
     3121 ctggtggctg gctcattccc tcactacagg acagacgggc tacatcccca gtaactatgt
     3181 cgcgccctca gactccatcc aggctgaaga gtggtacttt gggaagatca ctcgtcggga
     3241 gtccgagcgg ctgctgctca accccgaaaa cccccgggga accttcttgg tccgggagag
     3301 cgagacgaca aaaggtgcct attgcctctc cgtttctgac tttgacaacg ccaaggggct
     3361 caatgtgaag cactacaaga tccgcaagct ggacagcggc ggcttctaca tcacctcacg
     3421 cacacagttc agcagcctgc agcagctggt ggcctactac tccaaacatg ctgatggctt
     3481 gtgccaccgc ctgaccaacg tctgccccac gtccaagccc cagacccagg gactcgccaa
     3541 ggacgcgtgg gaaatccccc gggagtcgct gcggctggag gtgaagctgg ggcagggctg
     3601 ctttggagag gtctggatgg ggacctggaa cggcaccacc agagtggcca taaagactct
     3661 gaagcccggc accatgtccc cggaggcctt cctgcaggaa gcccaagtga tgaagaagct
     3721 ccggcatgag aagctggttc agctgtacgc agtggtgtcg gaagagccca tctacatcgt
     3781 cactgagtac atgagcaagg ggagcctcct ggatttcctg aagggagaga tgggcaagta
     3841 cctgcggctg ccacagctcg tcgatatggc tgctcagatt gcatccggca tggcctatgt
     3901 ggggaggatg aactacgtgc accgagacct gcgggcggcc aacatcctgg tgggggagaa
     3961 cctggtgtgc aaggtggctg actttgggct ggcacgcctc atcgaggaca acgagtacac
     4021 agcacggcaa ggtgccaagt tccccatcaa gtggacagcc cccgaggcag ccctctatgg
     4081 ccggttcacc atcaagtcgg atgtctggtc cttcggcatc ctgctgactg agctgaccac
     4141 caagggccgg gtgccatacc cagggatggt caacagggag gtgctggacc aggtggagag
     4201 gggctaccgc atgccctgcc cgcccgagtg ccccgagtcg ctgcatgacc tcatgtgcca
     4261 gtgctggcgg aaggaccctg aggagcggcc cacttttgag tacctgcagg ccttcctgga
     4321 ggactacttc acctcgacag agccccagta ccagcctgga gagaacctat agtgactcga
     4381 gatatctaga cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct
     4441 cgagtctaga gggcccgcgg ttcgaatacc catacgacgt cccagactac gcttagtaat
     4501 gattaattaa actagaaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     4561 gtaacccacc caagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     4621 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
     4681 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
     4741 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
     4801 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
     4861 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
     4921 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
     4981 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
     5041 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     5101 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     5161 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     5221 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     5281 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
     5341 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
     5401 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
     5461 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
     5521 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
     5581 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
     5641 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
     5701 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
     5761 ttctggccgt ttttggcttt tttgttagac gaagcttggt accgagctcg gatctccacc
     5821 ccgtaccggt cctgcagtcg aattcaccat gtctagactg gacaagagca aagtcataaa
     5881 cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc
     5941 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
     6001 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
     6061 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct
     6121 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
     6181 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
     6241 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
     6301 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
     6361 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
     6421 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc
     6481 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
     6541 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag
     6601 taaggatccg cggccgcact agaggaattc cgcccctctc cccccccccc ctctccctcc
     6661 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     6721 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     6781 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     6841 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     6901 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     6961 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     7021 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     7081 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     7141 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     7201 ctttgaaaaa cacgatgata agcttaccgg tccaccatga ttgaacaaga tggattgcac
     7261 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     7321 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     7381 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     7441 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     7501 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     7561 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     7621 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     7681 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     7741 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     7801 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     7861 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     7921 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     7981 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg atgtacaagt
     8041 aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     8101 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     8161 tgtttagtcc ctcccaattc gatatcaagc ttatcgcgat agatcctaat caacctctgg
     8221 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     8281 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     8341 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     8401 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     8461 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     8521 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     8581 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     8641 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8701 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     8761 agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac
     8821 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     8881 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     8941 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     9001 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     9061 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     9121 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg
     9181 gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat
     9241 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     9301 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     9661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    10021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    10081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    10141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    10201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    10261 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    10321 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10381 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10441 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10501 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10561 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10621 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10681 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10741 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10801 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10861 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10921 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10981 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    11041 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    11101 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    11161 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    11221 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg
    11281 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    11341 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg
    11401 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    11461 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    11521 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata
    11581 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    11641 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
    11701 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
    11761 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
