LOCUS       sequence-471980-       11473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral vector for 2xHA HCAR3 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Pedro MP, Lund K, Kang SWS, Chen T, Stuelten CH, Porat-Shliom N, 
            Iglesias-Bartolome R
  TITLE     GPCR Screening Reveals that the Metabolite Receptor HCAR3 Regulates 
            Epithelial Proliferation, Migration, and Cellular Respiration.
  JOURNAL   J Invest Dermatol. 2024 Jun;144(6):1311-1321.e7. doi: 
            10.1016/j.jid.2023.12.002. Epub 2023 Dec 14.
  PUBMED    38103827
REFERENCE   2  (bases 1 to 11473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.12.002"; journalName: "J Invest Dermatol"; date:
            "2024-06"; volume: "144"; issue: "6"; pages: "1311-1321.e7"
COMMENT     Sequence Label: pInducer20 2xHA  HCAR3
FEATURES             Location/Qualifiers
     source          1..11473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2780..2806
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2810..2836
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(4060..4084)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4137..4163
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4249..5460
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5297..5316
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5519..6136,6137..6142,6143..6178,6179..6181,6182..6217,
                     6218..6220,6221..6256,6257..6265)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6303..6876
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6470..6487)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6697..6716
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6892..7686
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6892..7686
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6946..6965)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7556..7575
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7863..8451
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7916..7936)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8334..8345)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8526..8759
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8878..8896)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8964..9068
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9069..9137,9138..9929)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9287..9306)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10100..10688
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10589..10608
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10842..10859
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11099..11120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11135..11140,11141..11158,11159..11165)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11173..11189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11178..11200
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11197..11213
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11197..11213
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11263..11282
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatccggtac
     2761 cgctagcgcc gccaccatgt acccatacga tgttcctgac tatgcgggct atccctatga
     2821 cgtcccggac tatgcaggat ccggagatcc acctgtcgca accatgaacc ggcaccacct
     2881 ccaggaccac ttcctggaga ttgacaaaaa gaactgctgt gtgttcaggg acgatttcat
     2941 cgccaaggta ctcccccctg tgcttgggtt ggagttcatc ttcgggctgc ttggcaatgg
     3001 gcttgcattg tggatttttt gcttccatct taagtcatgg aagtccagtc ggattttctt
     3061 gttcaatttg gccgtagcgg atttcctcct cattatctgc cttccctttg taatggatta
     3121 ctacgtgaga cgctctgatt ggaaattcgg ggatatcccg tgccggctgg tactcttcat
     3181 gtttgcgatg aacaggcaag ggagcatcat tttcttgact gtggtcgccg ttgatcgcta
     3241 tttcagagtg gttcatcctc accacgctct taataagata tccaactgga cagcagctat
     3301 tatctcatgt ttgttgtggg ggataacggt cgggctgact gtccatcttt tgaagaaaaa
     3361 gttgttgata cagaatggga cggctaatgt atgtatatcc ttctctattt gccatacgtt
     3421 tcgctggcac gaagctatgt ttctcttgga atttttcctg cctctgggta taatcttgtt
     3481 ctgctccgcc agaataattt ggtcattgag acagagacag atggacaggc acgcgaagat
     3541 taaacgggca atcacgttca taatggtggt agcaatcgta tttgtcatct gcttcctgcc
     3601 gtcagtggtt gtcaggattc acatcttctg gctcctgcac accagcggga ctcagaactg
     3661 cgaagtatat cgatcagtgg acctggcatt cttcatcacg ctcagtttta cgtacatgaa
     3721 cagcatgctt gaccctgtcg tgtattattt ttcaagcccc tcattcccga acttttttag
     3781 cacactgatt aatcgctgcc tccaaaggaa gataacaggc gaacccgata ataaccggtc
     3841 cacctctgtg gaactcacgg gagaccccaa caagaccagg ggtgcccccg aggcgctcat
     3901 tgcgaactct ggggaaccct ggtcccctag ctaccttgga ccaacgtcaa ataatcatag
     3961 taaaaagggg cactgtcatc aggagccagc cagtcttgaa aagcagcttg gctgttgtat
     4021 cgaataataa gaattcgcgg ccgcactcga gatatctaga cccagctttc ttgtacaaag
     4081 tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaatacc
     4141 catacgacgt cccagactac gcttagtaat gattaattaa actagaaatt ctaccgggta
     4201 ggggaggcgc ttttcccaag gcagtctgga gtaacccacc caagatctgg cctccgcgcc
     4261 gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg
     4321 aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct
     4381 cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg
     4441 gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc
     4501 ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg
     4561 acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt
     4621 ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt
     4681 cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag
     4741 tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc
     4801 ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac
     4861 aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg
     4921 aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt
     4981 gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc
     5041 gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc
     5101 gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt
     5161 ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg
     5221 gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc
     5281 tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg
     5341 ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt
     5401 cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac
     5461 gaagcttggt accgagctcg gatctccacc ccgtaccggt cctgcagtcg aattcaccat
     5521 gtctagactg gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg
     5581 tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct
     5641 gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga
     5701 caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa
     5761 caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct
     5821 cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg
     5881 tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac
     5941 actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc
     6001 taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg
     6061 agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct
     6121 aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc
     6181 agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt
     6241 tgaccttgac atgctccccg ggtaactaag taaggatccg cggccgcact agaggaattc
     6301 cgcccctctc cccccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     6361 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     6421 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     6481 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     6541 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     6601 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     6661 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     6721 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     6781 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     6841 ggggacgtgg ttttcctttg aaaaacacga tgataagctt accggtccac catgattgaa
     6901 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     6961 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     7021 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     7081 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     7141 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     7201 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     7261 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     7321 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     7381 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     7441 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     7501 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     7561 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     7621 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     7681 ttctgatgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     7741 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     7801 gaatgcaatt gttgttgttt agtccctccc aattcgatat caagcttatc gcgatagatc
     7861 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7921 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7981 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     8041 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     8101 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     8161 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     8221 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     8281 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     8341 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     8401 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     8461 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8521 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8581 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8641 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8701 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8761 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8821 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     8881 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8941 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9001 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9061 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9121 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9181 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9241 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9301 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9361 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9421 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9481 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9541 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9601 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9661 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9721 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9781 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9841 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9901 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9961 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10021 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10081 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10141 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10201 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10261 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10321 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10381 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10441 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10501 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10561 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10621 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10681 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10741 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10801 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10861 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10921 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    10981 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    11041 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    11101 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    11161 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
    11221 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11281 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    11341 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    11401 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    11461 caaacttagt agt
//
