LOCUS       sequence-471981-       10611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral vector for expression of optimized 
            2xHA-TEAD inhibitor (TEADiv2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Branch B, Yuan Y, Cascone M, Raimondi F, Iglesias-Bartolome R
  TITLE     An improved TEAD dominant-negative protein inhibitor to study Hippo 
            YAP1/TAZ-dependent transcription.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 3:2024.10.03.615022. doi: 
            10.1101/2024.10.03.615022.
  PUBMED    39502361
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 3:2024.10.03.615022. doi: 
            10.1101/2024.10.03.615022."
COMMENT     Sequence Label: pInducer20-HA-TEADiv2
FEATURES             Location/Qualifiers
     source          1..10611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2782..2808
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2812..2838
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3133..3153
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3198..3222)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3275..3301
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3387..4598
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4435..4454
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4657..5274,5275..5280,5281..5316,5317..5319,5320..5355,
                     5356..5358,5359..5394,5395..5403)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5441..6014
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5608..5625)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5835..5854
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6030..6824
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6030..6824
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6084..6103)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6694..6713
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7001..7589
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7054..7074)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7472..7483)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7664..7897
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8016..8034)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8102..8206
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8207..8275,8276..9067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8425..8444)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9238..9826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9727..9746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9980..9997
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10237..10258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10273..10278,10279..10296,10297..10303)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10311..10327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10316..10338
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10335..10351
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10335..10351
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10401..10420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctcttta aaggaaccaa ttcagtcgac ggccattgtg
     2761 gccatcaagc ttgccatggc ctacccatac gatgttcctg actatgcggg ctatccctat
     2821 gacgtcccgg actatgcagg atcctcagta gacgaccact ttgcgaaagc actgggtgat
     2881 acatggctgc aaataggtga cccccctgtc gcgaccaatc ccaaaacggc caatgttccg
     2941 caaacagtgc ccatgcggtt gcggaagttg ccggagtctt ttttcaaaga gcccgaggga
     3001 gaccctccag tggcgacgaa ccccaaaccc tcttcatggc gaaaaaagat tctgcccgag
     3061 agctttttta aagaaccggg ggacccgcca gttgcgacaa agcgaacagc ggatggcagt
     3121 gagttcgagt ccccgaagaa aaagcgcaag gttgagtaag cggccgcact cgagatatcg
     3181 gcctccaggg cctctagacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg
     3241 cggccgctcg agtctagagg gcccgcggtt cgaataccca tacgacgtcc cagactacgc
     3301 ttagtaatga ttaattaaac tagaaattct accgggtagg ggaggcgctt ttcccaaggc
     3361 agtctggagt aacccaccca agatctggcc tccgcgccgg gttttggcgc ctcccgcggg
     3421 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
     3481 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     3541 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     3601 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     3661 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     3721 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     3781 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
     3841 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
     3901 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
     3961 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
     4021 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
     4081 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
     4141 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
     4201 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
     4261 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt
     4321 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
     4381 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
     4441 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
     4501 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
     4561 ccgctaaatt ctggccgttt ttggcttttt tgttagacga agcttggtac cgagctcgga
     4621 tctccacccc gtaccggtcc tgcagtcgaa ttcaccatgt ctagactgga caagagcaaa
     4681 gtcataaacg gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg
     4741 aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag
     4801 cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc
     4861 cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt
     4921 gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa
     4981 cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag
     5041 aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa
     5101 caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca
     5161 cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc
     5221 ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg
     5281 accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt
     5341 gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg
     5401 taactaagta aggatccgcg gccgcactag aggaattccg cccctctccc cccccccccc
     5461 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     5521 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     5581 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     5641 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     5701 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     5761 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     5821 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     5881 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     5941 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     6001 aaacacgatg ataagcttac cggtccacca tgattgaaca agatggattg cacgcaggtt
     6061 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6121 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     6181 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     6241 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6301 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6361 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     6421 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     6481 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6541 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6601 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6661 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6721 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6781 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgatgtaca agtaaagcgg
     6841 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     6901 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgtttag
     6961 tccctcccaa ttcgatatca agcttatcgc gatagatcct aatcaacctc tggattacaa
     7021 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7081 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7141 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7201 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7261 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7321 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7381 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7441 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7501 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7561 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag
     7621 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7681 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7741 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7801 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7861 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7921 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt
     7981 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     8041 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     8101 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8161 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8221 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8281 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8341 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8401 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     8461 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8521 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8581 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8641 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8701 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8761 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8821 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8881 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8941 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9001 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9061 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9121 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9181 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9241 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9301 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9361 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9421 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9481 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9541 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9601 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9661 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9721 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9781 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9841 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9901 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9961 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    10021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
    10081 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    10141 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
    10201 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    10261 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    10321 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
    10381 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10441 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    10501 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    10561 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
