LOCUS       sequence-471990-       11508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid 
            with stability. L1-RSF1010-Tc-par-ELT4; GG assembly from pOGG004, 
            pOGG042, pNDGG005, pOGG012, and pOGG014, TcR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Geddes BA, Williamson R, Schumacher J, Ardi A, Levin G, Cervenka E, 
            Huang R, diCenzo G
  TITLE     BEVA2.0: modular assembly of golden gate-compatible vectors with 
            expanded utility for genetic engineering.
  JOURNAL   Can J Microbiol. 2025 Jan 1;71:1-13. doi: 10.1139/cjm-2024-0246.
  PUBMED    39919271
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Can J 
            Microbiol."; date: "2025-01-1"; volume: "71:1-13. doi"; pages: " 
            10.1139/cjm-2024-0246"
COMMENT     Sequence Label: pNDGG059
FEATURES             Location/Qualifiers
     source          1..11508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             798..1637
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             1624..2475
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDALCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     terminator      complement(5058..5144)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    5232..5253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5268..5273,5274..5291,5292..5298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5306..5322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5311..5333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5330..5346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5330..5346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5342..5665
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    5359..5415
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5416..5433)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5416..5432)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5425..5447)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5641..5660)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     terminator      5770..5864
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(5873..6523)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             6629..7828
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     oriT            7968..8076
                     /note="incP origin of transfer"
     rep_origin      8763..9157
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            9499..9585
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             join(10824..11508,1..287)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLASFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEETGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 ccgggctggt caagcgcttc ggtgatgacc tcagcaagtg cgactttatc gccgcgcaga
       61 agctggccag ccggggccgc agtgccgagg aaaccggcaa ggccatggcc gaggccagcc
      121 cagcgctggc agagcgcaag cccggccacg aagcggatta catcgagcgc accgtcagca
      181 aggtcatggg tctgcccagc gtccagcttg cgcgggccga gctggcacgg gcaccggcac
      241 cccgccagcg aggcatggac aggggcgggc cagatttcag catgtagtgc ttgcgttggt
      301 actcacgcct gttatactat gagtactcac gcacagaagg gggttttatg gaatacgaaa
      361 aaagcgcttc agggtcggtc tacctgatca aaagtgacaa gggctattgg ttgcccggtg
      421 gctttggtta tacgtcaaac aaggccgagg ctggccgctt ttcagtcgct gatatggcca
      481 gccttaacct tgacggctgc accttgtcct tgttccgcga agacaagcct ttcggccccg
      541 gcaagtttct cggtgactga tatgaaagac caaaaggaca agcagaccgg cgacctgctg
      601 gccagccctg acgctgtacg ccaagcgcga tatgccgagc gcatgaaggc caaagggatg
      661 cgtcagcgca agttctggct gaccgacgac gaatacgagg cgctgcgcga gtgcctggaa
      721 gaactcagag cggcgcaggg cgggggtagt gaccccgcca gcgcctaacc accaactgcc
      781 tgcaaaggag gcaatcaatg gctacccata agcctatcaa tattctggag gcgttcgcag
      841 cagcgccgcc accgctggac tacgttttgc ccaacatggt ggccggtacg gtcggggcgc
      901 tggtgtcgcc cggtggtgcc ggtaaatcca tgctggccct gcaactggcc gcacagattg
      961 caggcgggcc ggatctgctg gaggtgggcg aactgcccac cggcccggtg atctacctgc
     1021 ccgccgaaga cccgcccacc gccattcatc accgcctgca cgcccttggg gcgcacctca
     1081 gcgccgagga acggcaagcc gtggctgacg gcctgctgat ccagccgctg atcggcagcc
     1141 tgcccaacat catggccccg gagtggttcg acggcctcaa gcgcgccgcc gagggccgcc
     1201 gcctgatggt gctggacacg ctgcgccggt tccacatcga ggaagaaaac gccagcggcc
     1261 ccatggccca ggtcatcggt cgcatggagg ccatcgccgc cgataccggg tgctctatcg
     1321 tgttcctgca ccatgccagc aagggcgcgg ccatgatggg cgcaggcgac cagcagcagg
     1381 ccagccgggg cagctcggta ctggtcgata acatccgctg gcagtcctac ctgtcgagca
     1441 tgaccagcgc cgaggccgag gaatggggtg tggacgacga ccagcgccgg ttcttcgtcc
     1501 gcttcggtgt gagcaaggcc aactatggcg caccgttcgc tgatcggtgg ttcaggcggc
     1561 atgacggcgg ggtgctcaag cccgccgtgc tggagaggca gcgcaagagc aagggggtgc
     1621 cccgtggtga agcctaagaa caagcacagc ctcagccacg tccggcacga cccggcgcac
     1681 tgtctggccc ccggcctgtt ccgtgccctc aagcggggcg agcgcaagcg cagcaagctg
     1741 gacgtgacgt atgactacgg tgacggcaag cggatcgagt tcagcggccc ggagccgctg
     1801 ggcgctgatg atctgcgcat cctgcaaggg ctggtggcca tggctgggcc taatggccta
     1861 gtgcttggcc cggaacccaa gaccgaaggc ggacggcagc tccggctgtt cctggaaccc
     1921 aagtgggagg ccgtcaccgc tgatgccatg gtggtcaaag gtagctatcg ggcgctggca
     1981 aaggaaatcg gggcagaggt cgatagtggt ggggcgctca agcacataca ggactgcatc
     2041 gagcgccttt ggaaggtatc catcatcgcc cagaatggcc gcaagcggca ggggtttcgg
     2101 ctgctgtcgg agtacgccag cgacgaggcg gacgggcgcc tgtacgtggc cctgaacccc
     2161 ttgatcgcgc aggccgtcat gggtggcggc cagcatgtgc gcatcagcat ggacgaggtg
     2221 cgggcgctgg acagcgaaac cgcccgcctg ctgcaccagc ggctgtgtgg ctggatcgac
     2281 cccggcaaaa ccggcaaggc ttccatagat gccttgtgcg gctatgtctg gccgtcagag
     2341 gccagtggtt cgaccatgcg caagcgccgc cagcgggtgc gcgaggcgtt gccggagctg
     2401 gtcgcgctgg gctggacggt aaccgagttc gcggcgggca agtacgacat cacccggccc
     2461 aaggcggcag gctgaccccc cccactctat tgtaaacaag acatttttat cttttatatt
     2521 caatggctta ttttcctgct aattggtaat accatgaaaa ataccatgct cagaaaaggc
     2581 ttaacaatat tttgaaaaat tgcctactga gcgctgccgc acagctccat aggccgttac
     2641 tgcatgagct tgtggaagtg tgctgagcat aaatgattct cctagctgtt ctttgggtaa
     2701 gtacgccatc aggacgttgt gagtggcgcg atttttagcg gctgaaatca gcccttgagc
     2761 ctgtcggcaa gtcgcgtcat gaggtccatg cgctcatgca ggatcgccac gaccaacgcg
     2821 ggttcgcccg cacgcggcag gcaaaaaacg tagtggtgtt cgcagcgggc catccgcagc
     2881 gcgggaaaga gttcgctcat gtccttaaac gggccttcgc cggcggcaag cctggctatg
     2941 ccctgttcca gcttagcgat atagcggcgc acctgcgccg cgccccactc ccggcgcgtg
     3001 tagcggatga tgccgcgtag atcggcttcg gcctcagccg tgaggatgta ggccgtcaag
     3061 cgcgatcccc gctgagttct tcatcaagaa tttcgccgac gctcttggtg gacaccttgc
     3121 cggcaagccc atcgttgatg cggttcccca gcatggtttt cagttcctgc catgcctgat
     3181 cggcatcagc gtcaccgggg aacagacgtt cgagggcgta ttgcttaatg gtcttgccct
     3241 gcaaggcggc cagggctttc aggctctggt gctgctggtc cgtcatgtcg attgtcaggc
     3301 ggctcattgg ataacctcca taaaatacac gtaaccacat tagcacatat gtgggcgtga
     3361 ggctacagcg cgaggcgcat taaggtcggg aaaatgcgct aggcgcattt aaattgcgta
     3421 ttgctgtaat gcgccatgcc ggctagacta ggcccaaatg ggtataccca atttgaccaa
     3481 gggggacgcg atgagggcgg ccaagcacta ccgacaactt ctatccatcg acttcaacat
     3541 cgaggcgctg gccttcgtgc ctggacccga cggcacacgc ggccggcgca tccacgtcct
     3601 ggggcgcgag gtccgcgacc ggcccggcct ggtcgagtac ctttcgccgg cgttcggctc
     3661 gcgggtggcg ctggacggct actgcaaggc caatttcgat gcagtgctgc acctggcgta
     3721 ccccgatcat cagcaatggg gccacgcatg aagcgccgaa gctacgccat gctgcgcgcc
     3781 gctgccgcgc tggccgtcct ggtcgttgcc tcgccggcat gggccgagct gcgcggcgag
     3841 gtcgtgcgca tcatcgacgg cgacaccatc gacgtgctgg tagacaagca gccggtgcgc
     3901 gtgcgcctgg tggacattga cgcgccggaa aagcggcaag ccttcggcga acgtgcgcgc
     3961 caggcgctgg ccggcatggt gttccgccgg cacgtcctgg tcgacgagaa ggacaccgac
     4021 cgttacggcc gcacgctggg caccgtctgg gtcaacatgg agctggccag ccggccgccg
     4081 cagccgcgca acgtcaacgc cgcgatggtt caccagggca tggcgtgggc ctatcgcttc
     4141 cacggccgcg cggccgaccc tgaaatgctg cggctcgaac aggaggcgcg aggcaagcgc
     4201 gtcggcctct ggtccgatcc gcacgccgtc gagccgtgga aatggcgacg cgagagcaac
     4261 aaccggaggg acgaaggttg aaggtcgccc gcatctacct gcgcgccagt acggacgagc
     4321 agaatcttga acgccaggag agccttgtag cggccacgcg ggccgccggg tactacgtcg
     4381 ccggcatcta ccgcgagaag gcgtccggcg cacgcgccga ccggcccgag ctgctgcgca
     4441 tgatcgcgga cctgcaacct ggtgaagtcg tcgttgcgga gaagatcgac cgcatcagcc
     4501 gcttgccgtt ggccgaggcc gagcgcctgg ttgcgtcgat ccgggccaaa ggggccaagc
     4561 tggccgtgcc tggcgtggtg gacctgtcgg agctggccgc cgaggcgaac ggagtggcga
     4621 aaatcgttct ggaatccgtc caggacatgc ttttgaagct cgccttgcag atggcccgcg
     4681 acgactacga ggatcggcgc gagcgtcaac gtcagggtgt ccagttggcg aaggccgccg
     4741 gccgctacac cggccgcaaa cgtgacgccg gcatgcacga ccgcatcatc acgcttcgct
     4801 ccggcggatc gagcattgcc aagacggcca agctggtcgg atgcagcccg agccaggtca
     4861 aacgagtgtg ggcggcctgg aacgcgcagc agcaaaaata aagccgggca gtgcccggct
     4921 tttctcacct tttcgcgtcc cgcagggccg ctgcgagcgc cctacctaga tcctcgcttt
     4981 ccccctcggt gtagtccggc cagggcacga agggcgcgga tgcgaacctg ttgagcaggt
     5041 cagatctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     5101 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct gccgaattcg
     5161 gatccggagt gagaccgcag ctggcacgac aggtttgccg actggaaagc gggcagtgag
     5221 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     5281 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     5341 tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg atccccgggt
     5401 accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5461 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5521 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     5581 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     5641 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     5701 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgacggtctc
     5761 acgctcttgg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
     5821 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaggc aatcaatcgt
     5881 caccctttct cggtccttca acgttcctga caacgagcct ccttttcgcc aatccatcga
     5941 caatcaccgc gagtccctgc tcgaacgctg cgtccggacc ggcttcgtcg aaggcgtcta
     6001 tcgcggcccg caacagcggc gagagcggag cctgttcaac ggtgccgccg cgctcgccgg
     6061 catcgctgtc gccggcctgc tcctcaagca cggccccaac agtgaagtag ctgattgtca
     6121 tcagcgcatt gacggcgtcc ccggccgaaa aacccgcctc gcagaggaag cgaagctgcg
     6181 cgtcggccgt ttccatctgc ggtgcgcccg gtcgcgtgcc ggcatggatg cgcgcgccat
     6241 cgcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc
     6301 agtcgtcgtc ggctctcggc accgaatgcg tatgattctc cgccagcatg gcttcggcca
     6361 gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta aagcgccggc tgctgaaccc
     6421 ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac gccgacctcg ttcaacaggt
     6481 ccagggcggc acggatcact gtattcggct gcaactttgt catgcttgac actttatcac
     6541 tgataaacat aatatgtcca ccaacttatc agtgataaag aatccgcgcg ttcaatcgga
     6601 ccagcggagg ctggtccgga ggccagacgt gaaacccaac atacccctga tcgtaattct
     6661 gagcactgtc gcgctcgacg ctgtcggcat cggcctgatt atgccggtgc tgccgggcct
     6721 cctgcgcgat ctggttcact cgaacgacgt caccgcccac tatggcattc tgctggcgct
     6781 gtatgcgttg gtgcaatttg cctgcgcacc tgtgctgggc gcgctgtcgg atcgtttcgg
     6841 gcggcggcca atcttgctcg tgtcgctggc cggcgccact gtcgactacg ccatcatggc
     6901 gacagcgcct ttcctttggg ttctctatat cgggcggatc gtggccggca tcaccggggc
     6961 gactggggcg gtagccggcg cttatattgc cgatatcact gatggcgatg agcgcgcgcg
     7021 gcacttcggc ttcatgagcg cctgtttcgg gttcgggatg gtcgcgggac ctgtgctcgg
     7081 tgggctgatg ggcggtttct ccccccacgc tccgttcttc gccgcggcag ccttgaacgg
     7141 cctcaatttc ctgacgggct gtttcctttt gccggagtcg cacaaaggcg aacgccggcc
     7201 gttacgccgg gaggctctca acccgctcgc ttcgttccgg tgggcccggg gcatgaccgt
     7261 cgtcgccgcc ctgatggcgg tgttcttcat catgcaactt gtcggacagg tgccggccgc
     7321 gctttgggtc attttcggcg aggatcgctt tcactgggac gcgaccacga tcggcatttc
     7381 gcttgccgca tttggcattc tgcattcact cgcccaggca atgatcaccg gccctgtagc
     7441 cgcccggctc ggcgaaaggc gggcactcat gctcggaatg attgccgacg gcacaggcta
     7501 catcctgctt gccttcgcga cacggggatg gatggcgttc ccgatcatgg tcctgcttgc
     7561 ttcgggtggc atcggaatgc cggcgctgca agcaatgttg tccaggcagg tggatgagga
     7621 acgtcagggg cagctgcaag gctcactggc ggcgctcacc agcctgacct cgatcgtcgg
     7681 acccctcctc ttcacggcga tctatgcggc ttctataaca acgtggaacg ggtgggcatg
     7741 gattgcaggc gctgccctct acttgctctg cctgccggcg ctgcgtcgcg ggctttggag
     7801 cggcgcaggg caacgagccg atcgctgaac tacttttccg ctgcataacc ctgcttcggg
     7861 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
     7921 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
     7981 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
     8041 gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg ccggccgcga tcaagcaggt
     8101 gcgacagacg tcatactaga tatcaagcga cttctcctat cccctgggaa cacatcaatc
     8161 tcaccggaga atatcgctgg ccaaagcctt agcgtaggat tccgcccctt cccgcaaacg
     8221 accccaaaca ggaaacgcag ctgaaacggg aagctcaaca cccactgacg catgggttgt
     8281 tcaggcagta cttcatcaac cagcaaggcg gcactttcgg ccatccgccg cgccccacag
     8341 ctcgggcaga aaccgcgacg cttacagctg aaagcgacca ggtgctcggc gtggcaagac
     8401 tcgcagcgaa cccgtagaaa gccatgctcc agccgcccgc attggagaaa ttcttcaaat
     8461 tcccgttgca catagcccgg caattccttt ccctgctctg ccataagcgc agcgaatgcc
     8521 gggtaatact cgtcaacgat ctgatagaga agggtttgct cgggtcggtg gctctggtaa
     8581 cgaccagtat cccgatcccg gctggccgtc ctggccgcca catgaggcat gttccgcgtc
     8641 cttgcaatac tgtgtttaca tacagtctat cgcttagcgg aaagttcttt taccctcagc
     8701 cgaaatgcct gccgttgcta gacattgcca gccagtgccc gtcactcccg tactaactgt
     8761 cacgaacccc tgcaataact gtcacgcccc cctgcaataa ctgtcacgaa cccctgcaat
     8821 aactgtcacg cccccaaacc tgcaaaccca gcaggggcgg gggctggcgg ggtgttggaa
     8881 aaatccatcc atgattatct aagaataatc cactaggcgc ggttatcagc gcccttgtgg
     8941 ggcgctgctg cccttgccca atatgcccgg ccagaggccg ggtagctggt ctattcgctg
     9001 cgctaggcta cacaccgccc caccgctgcg cggcaggggg aaaggcgggc aaagcccgct
     9061 aaaccccaca ccaaaccccg cagaaatacg ctggagcgct tttagccgct ttagcggcct
     9121 ttccccctac ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat
     9181 cattcagccc ggctcatcct tctggcgtgg cggcagaccg aacaaggcgc ggtcgtggtc
     9241 gcgttcaagg tacgcatcca ttgccgccat gagccgatcc tccggccact cgctgctgtt
     9301 caccttggcc aaaatcatgg cccccaccag caccctgcgc cttgtttcgt tcttgcgctc
     9361 ttgctgctgt tcccttgccc gcacccgctg aatttcggca ttgattcgcg ctcgttgttc
     9421 ttcgagcttg gccagccgat ccgccgcctt gttgctcccc ttaaccatct tgacacccca
     9481 ttgttaatgt gctgtctcgt aggctatcat ggaggcacag cggcggcaat cccgacccta
     9541 ctttgtaggg gaggggcact taccggtttc tcttcgagaa actggcctaa cggccaccct
     9601 tcgggcggtg cgctctccga gggccattgc atggagccga aaagcaaaag caacagcgag
     9661 gcagcatggc gatttatcac cttacggcga aaaccggcag caggtcgggc ggccaatcgg
     9721 ccagggccaa ggccgactac atccagcgcg aaggcaagta tgcccgcgac atggatgaag
     9781 tcttgcacgc cgaatccggg cacatgccgg agttcgtcga gcggcccgcc gactactggg
     9841 atgctgccga cctgtatgaa cgcgccaatg ggcggctgtt caaggaggtc gaatttgccc
     9901 tgccggtcga gctgaccctc gaccagcaga aggcgctggc gtccgagttc gcccagcacc
     9961 tgaccggtgc cgagcgcctg ccgtatacgc tggccatcca tgccggtggc ggcgagaacc
    10021 cgcactgcca cctgatgatc tccgagcgga tcaatgacgg catcgagcgg cccgccgctc
    10081 agtggttcaa gcggtacaac ggcaagaccc cggagaaggg cggggcacgg aagaccgaag
    10141 cgctcaagcc caaggcatgg cttgagcaga cccgcgaggc atgggccgac catgccaacc
    10201 gggcattaga gcgggctggc cacgacgccc gcattgacca cagaacactt gaggcgcagg
    10261 gcatcgagcg cctgcccggt gttcacctgg ggccgaacgt ggtggagatg gaaggccggg
    10321 gcatccgcac cgaccgggca gacgtggccc tgaacatcga caccgccaac acccagatca
    10381 tcgacttaca ggaataccgg gaggcaatag accatgaacg caatcgacag agtgaagaaa
    10441 tccagaggca tcaacgagtt agcggagcag atcgaaccgc tggcccagag catggcgaca
    10501 ctggccgacg aagcccggca ggtcatgagc cagacccagc aggccagcga ggcgcaggcg
    10561 gcggagtggc tgaaagccca gcgccagaca ggggcggcat gggtggagct ggccaaagag
    10621 ttgcgggagg tagccgccga ggtgagcagc gccgcgcaga gcgcccggag cgcgtcgcgg
    10681 gggtggcact ggaagctatg gctaaccgtg atgctggctt ccatgatgcc tacggtggtg
    10741 ctgctgatcg catcgttgct cttgctcgac ctgacgccac tgacaaccga ggacggctcg
    10801 atctggctgc gcttggtggc ccgatgaaga acgacaggac tttgcaggcc ataggccgac
    10861 agctcaaggc catgggctgt gagcgcttcg atatcggcgt cagggacgcc accaccggcc
    10921 agatgatgaa ccgggaatgg tcagccgccg aagtgctcca gaacacgcca tggctcaagc
    10981 ggatgaatgc ccagggcaat gacgtgtata tcaggcccgc cgagcaggag cggcatggtc
    11041 tggtgctggt ggacgacctc agcgagtttg acctggatga catgaaagcc gagggccggg
    11101 agcctgccct ggtagtggaa accagcccga agaactatca ggcatgggtc aaggtggccg
    11161 acgccgcagg cggtgaactt cgggggcaga ttgcccggac gctggccagc gagtacgacg
    11221 ccgacccggc cagcgccgac agccgccact atggccgctt ggcgagcttc accaaccgca
    11281 aggacaagca caccacccgc gccggttatc agccgtgggt gctgctgcgt gaatccaagg
    11341 gcaagaccgc caccgctggc ccggcgctgg tgcagcaggc tggccagcag atcgagcagg
    11401 cccagcggca gcaggagaag gcccgcaggc tggccagcct cgaactgccc gagcggcagc
    11461 ttagccgcca ccggcgcacg gcgctggacg agtaccgcag cgagatgg
//
