LOCUS       sequence-47200-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3050-06
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(83..111)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     706..726
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctgcgggtcg acggatcccg ggcttaagat cggtgacggt gctggtttaa ttaacagtaa
       61 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      121 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      181 ccttaaattg atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      241 tttgggttat ggtctaatgt gctttgctag atacccagat catatgaaac ggcatgactt
      301 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
      361 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
      421 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
      481 caactataac tcacacaatg tatacatcac tgcagacaaa caaaagaatg gaatcaaagc
      541 taacttcaaa attagacaca acattgaaga tggaggtgtt caactagcag accattatca
      601 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccta
      661 tcaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
      721 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaattcg aacacgacga
      781 attgtagggc gcgccacttc taaataagcg aattt
//
