LOCUS       sequence-472017-       10588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid 
            with stability. L1-RSF1010-Km-par-ELT4; GG assembly from pOGG004, 
            pNDGG001, pNDGG005, pOGG012, and pOGG014, NmR/KmR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Geddes BA, Williamson R, Schumacher J, Ardi A, Levin G, Cervenka E, 
            Huang R, diCenzo G
  TITLE     BEVA2.0: modular assembly of golden gate-compatible vectors with 
            expanded utility for genetic engineering.
  JOURNAL   Can J Microbiol. 2025 Jan 1;71:1-13. doi: 10.1139/cjm-2024-0246.
  PUBMED    39919271
REFERENCE   2  (bases 1 to 10588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Can J 
            Microbiol."; date: "2025-01-1"; volume: "71:1-13. doi"; pages: " 
            10.1139/cjm-2024-0246"
COMMENT     Sequence Label: pNDGG057
FEATURES             Location/Qualifiers
     source          1..10588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             520..1359
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             1346..2197
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDALCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     terminator      complement(4780..4866)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    4954..4975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4990..4995,4996..5013,5014..5020)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5028..5044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5033..5055
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5052..5068
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5052..5068
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5064..5387
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    5081..5137
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5138..5155)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5138..5154)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5147..5169)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5363..5382)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     terminator      5492..5586
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             5836..6630
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5890..5909)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6500..6519
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     oriT            6770..6878
                     /note="incP origin of transfer"
     rep_origin      7565..7959
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            8301..8387
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             join(9626..10588,1..9)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLASFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEETGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 agcatgtagt gcttgcgttg gtactcacgc ctgttatact atgagtactc acgcacagaa
       61 gggggtttta tggaatacga aaaaagcgct tcagggtcgg tctacctgat caaaagtgac
      121 aagggctatt ggttgcccgg tggctttggt tatacgtcaa acaaggccga ggctggccgc
      181 ttttcagtcg ctgatatggc cagccttaac cttgacggct gcaccttgtc cttgttccgc
      241 gaagacaagc ctttcggccc cggcaagttt ctcggtgact gatatgaaag accaaaagga
      301 caagcagacc ggcgacctgc tggccagccc tgacgctgta cgccaagcgc gatatgccga
      361 gcgcatgaag gccaaaggga tgcgtcagcg caagttctgg ctgaccgacg acgaatacga
      421 ggcgctgcgc gagtgcctgg aagaactcag agcggcgcag ggcgggggta gtgaccccgc
      481 cagcgcctaa ccaccaactg cctgcaaagg aggcaatcaa tggctaccca taagcctatc
      541 aatattctgg aggcgttcgc agcagcgccg ccaccgctgg actacgtttt gcccaacatg
      601 gtggccggta cggtcggggc gctggtgtcg cccggtggtg ccggtaaatc catgctggcc
      661 ctgcaactgg ccgcacagat tgcaggcggg ccggatctgc tggaggtggg cgaactgccc
      721 accggcccgg tgatctacct gcccgccgaa gacccgccca ccgccattca tcaccgcctg
      781 cacgcccttg gggcgcacct cagcgccgag gaacggcaag ccgtggctga cggcctgctg
      841 atccagccgc tgatcggcag cctgcccaac atcatggccc cggagtggtt cgacggcctc
      901 aagcgcgccg ccgagggccg ccgcctgatg gtgctggaca cgctgcgccg gttccacatc
      961 gaggaagaaa acgccagcgg ccccatggcc caggtcatcg gtcgcatgga ggccatcgcc
     1021 gccgataccg ggtgctctat cgtgttcctg caccatgcca gcaagggcgc ggccatgatg
     1081 ggcgcaggcg accagcagca ggccagccgg ggcagctcgg tactggtcga taacatccgc
     1141 tggcagtcct acctgtcgag catgaccagc gccgaggccg aggaatgggg tgtggacgac
     1201 gaccagcgcc ggttcttcgt ccgcttcggt gtgagcaagg ccaactatgg cgcaccgttc
     1261 gctgatcggt ggttcaggcg gcatgacggc ggggtgctca agcccgccgt gctggagagg
     1321 cagcgcaaga gcaagggggt gccccgtggt gaagcctaag aacaagcaca gcctcagcca
     1381 cgtccggcac gacccggcgc actgtctggc ccccggcctg ttccgtgccc tcaagcgggg
     1441 cgagcgcaag cgcagcaagc tggacgtgac gtatgactac ggtgacggca agcggatcga
     1501 gttcagcggc ccggagccgc tgggcgctga tgatctgcgc atcctgcaag ggctggtggc
     1561 catggctggg cctaatggcc tagtgcttgg cccggaaccc aagaccgaag gcggacggca
     1621 gctccggctg ttcctggaac ccaagtggga ggccgtcacc gctgatgcca tggtggtcaa
     1681 aggtagctat cgggcgctgg caaaggaaat cggggcagag gtcgatagtg gtggggcgct
     1741 caagcacata caggactgca tcgagcgcct ttggaaggta tccatcatcg cccagaatgg
     1801 ccgcaagcgg caggggtttc ggctgctgtc ggagtacgcc agcgacgagg cggacgggcg
     1861 cctgtacgtg gccctgaacc ccttgatcgc gcaggccgtc atgggtggcg gccagcatgt
     1921 gcgcatcagc atggacgagg tgcgggcgct ggacagcgaa accgcccgcc tgctgcacca
     1981 gcggctgtgt ggctggatcg accccggcaa aaccggcaag gcttccatag atgccttgtg
     2041 cggctatgtc tggccgtcag aggccagtgg ttcgaccatg cgcaagcgcc gccagcgggt
     2101 gcgcgaggcg ttgccggagc tggtcgcgct gggctggacg gtaaccgagt tcgcggcggg
     2161 caagtacgac atcacccggc ccaaggcggc aggctgaccc cccccactct attgtaaaca
     2221 agacattttt atcttttata ttcaatggct tattttcctg ctaattggta ataccatgaa
     2281 aaataccatg ctcagaaaag gcttaacaat attttgaaaa attgcctact gagcgctgcc
     2341 gcacagctcc ataggccgtt actgcatgag cttgtggaag tgtgctgagc ataaatgatt
     2401 ctcctagctg ttctttgggt aagtacgcca tcaggacgtt gtgagtggcg cgatttttag
     2461 cggctgaaat cagcccttga gcctgtcggc aagtcgcgtc atgaggtcca tgcgctcatg
     2521 caggatcgcc acgaccaacg cgggttcgcc cgcacgcggc aggcaaaaaa cgtagtggtg
     2581 ttcgcagcgg gccatccgca gcgcgggaaa gagttcgctc atgtccttaa acgggccttc
     2641 gccggcggca agcctggcta tgccctgttc cagcttagcg atatagcggc gcacctgcgc
     2701 cgcgccccac tcccggcgcg tgtagcggat gatgccgcgt agatcggctt cggcctcagc
     2761 cgtgaggatg taggccgtca agcgcgatcc ccgctgagtt cttcatcaag aatttcgccg
     2821 acgctcttgg tggacacctt gccggcaagc ccatcgttga tgcggttccc cagcatggtt
     2881 ttcagttcct gccatgcctg atcggcatca gcgtcaccgg ggaacagacg ttcgagggcg
     2941 tattgcttaa tggtcttgcc ctgcaaggcg gccagggctt tcaggctctg gtgctgctgg
     3001 tccgtcatgt cgattgtcag gcggctcatt ggataacctc cataaaatac acgtaaccac
     3061 attagcacat atgtgggcgt gaggctacag cgcgaggcgc attaaggtcg ggaaaatgcg
     3121 ctaggcgcat ttaaattgcg tattgctgta atgcgccatg ccggctagac taggcccaaa
     3181 tgggtatacc caatttgacc aagggggacg cgatgagggc ggccaagcac taccgacaac
     3241 ttctatccat cgacttcaac atcgaggcgc tggccttcgt gcctggaccc gacggcacac
     3301 gcggccggcg catccacgtc ctggggcgcg aggtccgcga ccggcccggc ctggtcgagt
     3361 acctttcgcc ggcgttcggc tcgcgggtgg cgctggacgg ctactgcaag gccaatttcg
     3421 atgcagtgct gcacctggcg taccccgatc atcagcaatg gggccacgca tgaagcgccg
     3481 aagctacgcc atgctgcgcg ccgctgccgc gctggccgtc ctggtcgttg cctcgccggc
     3541 atgggccgag ctgcgcggcg aggtcgtgcg catcatcgac ggcgacacca tcgacgtgct
     3601 ggtagacaag cagccggtgc gcgtgcgcct ggtggacatt gacgcgccgg aaaagcggca
     3661 agccttcggc gaacgtgcgc gccaggcgct ggccggcatg gtgttccgcc ggcacgtcct
     3721 ggtcgacgag aaggacaccg accgttacgg ccgcacgctg ggcaccgtct gggtcaacat
     3781 ggagctggcc agccggccgc cgcagccgcg caacgtcaac gccgcgatgg ttcaccaggg
     3841 catggcgtgg gcctatcgct tccacggccg cgcggccgac cctgaaatgc tgcggctcga
     3901 acaggaggcg cgaggcaagc gcgtcggcct ctggtccgat ccgcacgccg tcgagccgtg
     3961 gaaatggcga cgcgagagca acaaccggag ggacgaaggt tgaaggtcgc ccgcatctac
     4021 ctgcgcgcca gtacggacga gcagaatctt gaacgccagg agagccttgt agcggccacg
     4081 cgggccgccg ggtactacgt cgccggcatc taccgcgaga aggcgtccgg cgcacgcgcc
     4141 gaccggcccg agctgctgcg catgatcgcg gacctgcaac ctggtgaagt cgtcgttgcg
     4201 gagaagatcg accgcatcag ccgcttgccg ttggccgagg ccgagcgcct ggttgcgtcg
     4261 atccgggcca aaggggccaa gctggccgtg cctggcgtgg tggacctgtc ggagctggcc
     4321 gccgaggcga acggagtggc gaaaatcgtt ctggaatccg tccaggacat gcttttgaag
     4381 ctcgccttgc agatggcccg cgacgactac gaggatcggc gcgagcgtca acgtcagggt
     4441 gtccagttgg cgaaggccgc cggccgctac accggccgca aacgtgacgc cggcatgcac
     4501 gaccgcatca tcacgcttcg ctccggcgga tcgagcattg ccaagacggc caagctggtc
     4561 ggatgcagcc cgagccaggt caaacgagtg tgggcggcct ggaacgcgca gcagcaaaaa
     4621 taaagccggg cagtgcccgg cttttctcac cttttcgcgt cccgcagggc cgctgcgagc
     4681 gccctaccta gatcctcgct ttccccctcg gtgtagtccg gccagggcac gaagggcgcg
     4741 gatgcgaacc tgttgagcag gtcagatcta gggcggcgga tttgtcctac tcaggagagc
     4801 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     4861 tatttgatgc ctgccgaatt cggatccgga gtgagaccgc agctggcacg acaggtttgc
     4921 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     4981 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     5041 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat gcctgcaggt
     5101 cgactctaga ggatccccgg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc
     5161 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     5221 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     5281 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     5341 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     5401 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5461 acagacaagc tgtgacggtc tcacgctctt ggactcctgt tgatagatcc agtaatgacc
     5521 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
     5581 gagaatccag gcaatatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct
     5641 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg
     5701 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
     5761 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga
     5821 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5881 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5941 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     6001 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     6061 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     6121 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     6181 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6241 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     6301 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     6361 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     6421 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     6481 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6541 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     6601 ttctatcgcc ttcttgacga gttcttctga actacttttc cgctgcataa ccctgcttcg
     6661 gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt
     6721 gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag
     6781 gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg
     6841 cggtgctcaa cgggaatcct gctctgcgag gctggccgta ggccggccgc gatcaagcag
     6901 gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg aacacatcaa
     6961 tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc ttcccgcaaa
     7021 cgaccccaaa caggaaacgc agctgaaacg ggaagctcaa cacccactga cgcatgggtt
     7081 gttcaggcag tacttcatca accagcaagg cggcactttc ggccatccgc cgcgccccac
     7141 agctcgggca gaaaccgcga cgcttacagc tgaaagcgac caggtgctcg gcgtggcaag
     7201 actcgcagcg aacccgtaga aagccatgct ccagccgccc gcattggaga aattcttcaa
     7261 attcccgttg cacatagccc ggcaattcct ttccctgctc tgccataagc gcagcgaatg
     7321 ccgggtaata ctcgtcaacg atctgataga gaagggtttg ctcgggtcgg tggctctggt
     7381 aacgaccagt atcccgatcc cggctggccg tcctggccgc cacatgaggc atgttccgcg
     7441 tccttgcaat actgtgttta catacagtct atcgcttagc ggaaagttct tttaccctca
     7501 gccgaaatgc ctgccgttgc tagacattgc cagccagtgc ccgtcactcc cgtactaact
     7561 gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat aactgtcacg aacccctgca
     7621 ataactgtca cgcccccaaa cctgcaaacc cagcaggggc gggggctggc ggggtgttgg
     7681 aaaaatccat ccatgattat ctaagaataa tccactaggc gcggttatca gcgcccttgt
     7741 ggggcgctgc tgcccttgcc caatatgccc ggccagaggc cgggtagctg gtctattcgc
     7801 tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg ggaaaggcgg gcaaagcccg
     7861 ctaaacccca caccaaaccc cgcagaaata cgctggagcg cttttagccg ctttagcggc
     7921 ctttccccct acccgaaggg tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg
     7981 atcattcagc ccggctcatc cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg
     8041 tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat cctccggcca ctcgctgctg
     8101 ttcaccttgg ccaaaatcat ggcccccacc agcaccctgc gccttgtttc gttcttgcgc
     8161 tcttgctgct gttcccttgc ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt
     8221 tcttcgagct tggccagccg atccgccgcc ttgttgctcc ccttaaccat cttgacaccc
     8281 cattgttaat gtgctgtctc gtaggctatc atggaggcac agcggcggca atcccgaccc
     8341 tactttgtag gggaggggca cttaccggtt tctcttcgag aaactggcct aacggccacc
     8401 cttcgggcgg tgcgctctcc gagggccatt gcatggagcc gaaaagcaaa agcaacagcg
     8461 aggcagcatg gcgatttatc accttacggc gaaaaccggc agcaggtcgg gcggccaatc
     8521 ggccagggcc aaggccgact acatccagcg cgaaggcaag tatgcccgcg acatggatga
     8581 agtcttgcac gccgaatccg ggcacatgcc ggagttcgtc gagcggcccg ccgactactg
     8641 ggatgctgcc gacctgtatg aacgcgccaa tgggcggctg ttcaaggagg tcgaatttgc
     8701 cctgccggtc gagctgaccc tcgaccagca gaaggcgctg gcgtccgagt tcgcccagca
     8761 cctgaccggt gccgagcgcc tgccgtatac gctggccatc catgccggtg gcggcgagaa
     8821 cccgcactgc cacctgatga tctccgagcg gatcaatgac ggcatcgagc ggcccgccgc
     8881 tcagtggttc aagcggtaca acggcaagac cccggagaag ggcggggcac ggaagaccga
     8941 agcgctcaag cccaaggcat ggcttgagca gacccgcgag gcatgggccg accatgccaa
     9001 ccgggcatta gagcgggctg gccacgacgc ccgcattgac cacagaacac ttgaggcgca
     9061 gggcatcgag cgcctgcccg gtgttcacct ggggccgaac gtggtggaga tggaaggccg
     9121 gggcatccgc accgaccggg cagacgtggc cctgaacatc gacaccgcca acacccagat
     9181 catcgactta caggaatacc gggaggcaat agaccatgaa cgcaatcgac agagtgaaga
     9241 aatccagagg catcaacgag ttagcggagc agatcgaacc gctggcccag agcatggcga
     9301 cactggccga cgaagcccgg caggtcatga gccagaccca gcaggccagc gaggcgcagg
     9361 cggcggagtg gctgaaagcc cagcgccaga caggggcggc atgggtggag ctggccaaag
     9421 agttgcggga ggtagccgcc gaggtgagca gcgccgcgca gagcgcccgg agcgcgtcgc
     9481 gggggtggca ctggaagcta tggctaaccg tgatgctggc ttccatgatg cctacggtgg
     9541 tgctgctgat cgcatcgttg ctcttgctcg acctgacgcc actgacaacc gaggacggct
     9601 cgatctggct gcgcttggtg gcccgatgaa gaacgacagg actttgcagg ccataggccg
     9661 acagctcaag gccatgggct gtgagcgctt cgatatcggc gtcagggacg ccaccaccgg
     9721 ccagatgatg aaccgggaat ggtcagccgc cgaagtgctc cagaacacgc catggctcaa
     9781 gcggatgaat gcccagggca atgacgtgta tatcaggccc gccgagcagg agcggcatgg
     9841 tctggtgctg gtggacgacc tcagcgagtt tgacctggat gacatgaaag ccgagggccg
     9901 ggagcctgcc ctggtagtgg aaaccagccc gaagaactat caggcatggg tcaaggtggc
     9961 cgacgccgca ggcggtgaac ttcgggggca gattgcccgg acgctggcca gcgagtacga
    10021 cgccgacccg gccagcgccg acagccgcca ctatggccgc ttggcgagct tcaccaaccg
    10081 caaggacaag cacaccaccc gcgccggtta tcagccgtgg gtgctgctgc gtgaatccaa
    10141 gggcaagacc gccaccgctg gcccggcgct ggtgcagcag gctggccagc agatcgagca
    10201 ggcccagcgg cagcaggaga aggcccgcag gctggccagc ctcgaactgc ccgagcggca
    10261 gcttagccgc caccggcgca cggcgctgga cgagtaccgc agcgagatgg ccgggctggt
    10321 caagcgcttc ggtgatgacc tcagcaagtg cgactttatc gccgcgcaga agctggccag
    10381 ccggggccgc agtgccgagg aaaccggcaa ggccatggcc gaggccagcc cagcgctggc
    10441 agagcgcaag cccggccacg aagcggatta catcgagcgc accgtcagca aggtcatggg
    10501 tctgcccagc gtccagcttg cgcgggccga gctggcacgg gcaccggcac cccgccagcg
    10561 aggcatggac aggggcgggc cagatttc
//
