LOCUS       sequence-472019-        9075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SRSF3 mCherry fusion protein with MCP domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Bhat P, Chow A, Emert B, Ettlin O, Quinodoz SA, Strehle M, Takei Y, 
            Burr A, Goronzy IN, Chen AW, Huang W, Ferrer JLM, Soehalim E, Goh 
            ST, Chari T, Sullivan DK, Blanco MR, Guttman M
  TITLE     Genome organization around nuclear speckles drives mRNA splicing 
            efficiency.
  JOURNAL   Nature. 2024 May;629(8014):1165-1173. doi: 
            10.1038/s41586-024-07429-6. Epub 2024 May 8.
  PUBMED    38720076
REFERENCE   2  (bases 1 to 9075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07429-6"; journalName: "Nature"; date: 
            "2024-05"; volume: "629"; issue: "8014"; pages: "1165-1173"
COMMENT     Sequence Label: pCAG-MCP-SRSF3-V5-mCherry
FEATURES             Location/Qualifiers
     source          1..9075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          335..1351
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1275..1297
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1359..1378
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1439..1448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2234..2258
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2751..2775)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2783..2824
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2825..3535
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2970..2988)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3238..3257
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(3622..4196,4197..4199,4200..4208)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3789..3806)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4016..4035
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4209..4808
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4209..4228)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4705..4725
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4889..4906)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4895..5119
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5166..5182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5166..5182)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5179..5201)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5190..5206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5214..5220,5221..5238,5239..5244))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5259..5280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5339..5534
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5385..5520
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5540..5674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5742..5759)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5913..6501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5993..6012)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6672..7463,7464..7532))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7295..7314
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     polyA_signal    8125..8259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        8273..8652
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tctgggaggc gccgccgcac cccctctagc skttttwryc cycscttttc ccykggksgg
       61 ggkgsccccc ccyttctcct yccccccccc cccccccccc cccccccccc ccccaatttt
      121 tttttttttt tttttttwat tttttkgggg gggggggggg gggggggggg ggggggggcg
      181 cgcgcsrggs ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      241 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      301 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
      361 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
      421 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
      481 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
      541 tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
      601 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
      661 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
      721 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
      781 gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg
      841 cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg
      901 ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggrgggctc
      961 gggggrgggg cgcggcggcc cccggrgcgc cggcggctgt cgaggcgcgg cgagccgcag
     1021 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     1081 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
     1141 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     1201 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
     1261 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     1321 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     1381 gtctcatcat tttggcaaag aattcctcga ggctagccgt cgaagaattc tcacgcgtgc
     1441 caccatggcc agcaacttca cccagttcgt gctggtggac aacggcggca ccggcgacgt
     1501 gaccgtggcc cccagcaact tcgccaacgg catcgccgag tggatcagca gcaacagccg
     1561 cagccaggcc tacaaggtga cctgcagcgt gcgccagagc agcgcccaga accgcaagta
     1621 caccatcaag gtggaggtgc ccaagggcgc ctggcgcagc tacctgaaca tggagctgac
     1681 catccccatc ttcgccacca acagcgactg cgagctgatc gtgaaggcca tgcagggcct
     1741 gctgaaggac ggcaacccca tccccagcgc catcgccgcc aacagcggca tctacggcgg
     1801 cgctggaggt gcaggtggat ccatggccag caacttcacc cagttcgtgc tggtggacaa
     1861 cggcggcacc ggcgacgtga ccgtggcccc cagcaacttc gccaacggca tcgccgagtg
     1921 gatcagcagc aacagccgca gccaggccta caaggtgacc tgcagcgtgc gccagagcag
     1981 cgcccagaac cgcaagtaca ccatcaaggt ggaggtgccc aagggcgcct ggcgcagcta
     2041 cctgaacatg gagctgacca tccccatctt cgccaccaac agcgactgcg agctgatcgt
     2101 gaaggccatg cagggcctgc tgaaggacgg caaccccatc cccagcgcca tcgccgccaa
     2161 cagcggcatc tacggcggcg ctggaggtgc aggtggatcc gtgagcaagg gcgaggagct
     2221 ggtcggcgcg ccaacaagtt tgtacaaaaa agttggcatg catcgtgatt cctgtccatt
     2281 ggactgtaag gtttatgtag gcaatcttgg aaacaatggc aacaagacgg aattggaacg
     2341 ggcttttggc tactatggac cactccgaag tgtgtgggtt gctagaaacc cacccggctt
     2401 tgcttttgtt gaatttgaag atccccgaga tgcagctgat gcagtccgag agctagatgg
     2461 aagaacacta tgtggctgcc gtgtaagagt ggaactgtcg aatggtgaaa aaagaagtag
     2521 aaatcgtggc ccacctccct cttggggtcg tcgccctcga gatgattatc gtaggaggag
     2581 tcctccacct cgtcgcagat ctccaagaag gagaagcttc tctcgcagcc ggagcaggtc
     2641 cctttctaga gataggagaa gagagagatc gctgtctcgg gagagaaatc acaagccgtc
     2701 ccgatccttc tctaggtctc gtagtcgatc taggtcaaat gaaaggaaat acccaacttt
     2761 cttgtacaaa gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc
     2821 tacgatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     2881 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     2941 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     3001 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3061 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     3121 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     3181 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     3241 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     3301 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     3361 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     3421 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     3481 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtagtaatg
     3541 aactagtacc ggttaagtcg acaatcaacg cgttaagtcg acaggccgct cgataagctt
     3601 gatatcgaat tccgcccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag
     3661 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     3721 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     3781 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     3841 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     3901 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     3961 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     4021 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     4081 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     4141 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     4201 ccacaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     4261 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     4321 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     4381 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     4441 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     4501 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     4561 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     4621 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     4681 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     4741 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     4801 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     4861 catcgatgat ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     4921 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     4981 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5041 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     5101 gatgcggtgg gctctatggc ttctgaggcg gaaagaacct gcagcccaag cttggcgtaa
     5161 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5221 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5281 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc
     5341 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5401 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5461 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5521 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa
     5581 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5641 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc
     5701 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5761 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5821 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5881 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5941 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6001 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6061 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6121 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6181 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6241 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6301 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6361 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6421 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6481 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6541 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6601 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6661 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6721 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6781 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6841 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6901 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6961 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7021 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7081 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7141 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7201 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7261 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7321 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7381 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7441 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7501 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaatg accgctttgg
     7561 aaaaacaaag actgtatttc ctggaaatta atgtttattc aataaactgt gtattcagct
     7621 atattcacat agtggtgagg ctgaaatgag gcgggaagag gcggttgggg cttaattata
     7681 tcaatttggg tggccccaca gcgcctccaa ggcgccagtc ctgttttgac aagttgcctc
     7741 tggaagcctc tcttcttttt ctccagagta agcggaggcc aggggccccc ggcctctgct
     7801 taatactaaa aaaaacagct gttgtcatag taatgattgg gtggaaacat tccaggcctg
     7861 ggtggagagg ctttttgctt cctcttgcaa aaccacactg acattccagg cctgggtgga
     7921 gaggcttttt gcttcctctt gcaaaaccac actgccctct ggagggcagt tgcctagcaa
     7981 ctaattaaaa gaggatgtcg cacggccagc tgcggtcagt tagtcacttc ctgcttaact
     8041 gacttgacat tttctatttt aagagtcggg aggaaaatta ctgtgttgga ggccctccgc
     8101 catcttctga agctgaatcg aattaacttg tttattgcag cttataatgg ttacaaataa
     8161 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8221 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatatcatcg tcgacattga
     8281 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     8341 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     8401 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     8461 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     8521 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     8581 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     8641 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     8701 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     8761 gggggggggg ggggggggsg ccsggggggg gggggggggg gggggggggg gggggggggg
     8821 gggggggggg gggggggggg ggcggggggg gggggggggg gggggggggg gggggggggg
     8881 gggggggggg gggggggggg gggggggggg gggggggggc sgcsgggggs gggggggggc
     8941 kggggggrgg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     9001 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     9061 tgtgcggagc cgaaa
//
