LOCUS       sequence-472024-        8847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SRSF1 mCherry fusion protein with MCP domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Bhat P, Chow A, Emert B, Ettlin O, Quinodoz SA, Strehle M, Takei Y, 
            Burr A, Goronzy IN, Chen AW, Huang W, Ferrer JLM, Soehalim E, Goh 
            ST, Chari T, Sullivan DK, Blanco MR, Guttman M
  TITLE     Genome organization around nuclear speckles drives mRNA splicing 
            efficiency.
  JOURNAL   Nature. 2024 May;629(8014):1165-1173. doi: 
            10.1038/s41586-024-07429-6. Epub 2024 May 8.
  PUBMED    38720076
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07429-6"; journalName: "Nature"; date: 
            "2024-05"; volume: "629"; issue: "8014"; pages: "1165-1173"
COMMENT     Sequence Label: pCAG-MCP-SRSF1-V5-mCherry
FEATURES             Location/Qualifiers
     source          1..8847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(170..961,962..1030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     793..812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     polyA_signal    1623..1757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        1771..2150
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2428..3444
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3368..3390
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3452..3471
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3532..3541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4327..4351
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5096..5120)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5128..5169
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5170..5880
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5315..5333)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5583..5602
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(5967..6541,6542..6544,6545..6553)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6134..6151)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6361..6380
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6554..7153
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6554..6573)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7050..7070
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7234..7251)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7240..7464
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7511..7527)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7511..7527)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7524..7546)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7535..7551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7559..7565,7566..7583,7584..7589))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7604..7625
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7684..7879
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7730..7865
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7885..8019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8087..8104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8258..8846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8338..8357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      841 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      901 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      961 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1021 gaatactcat actcttcctt tttcaatgac cgctttggaa aaacaaagac tgtatttcct
     1081 ggaaattaat gtttattcaa taaactgtgt attcagctat attcacatag tggtgaggct
     1141 gaaatgaggc gggaagaggc ggttggggct taattatatc aatttgggtg gccccacagc
     1201 gcctccaagg cgccagtcct gttttgacaa gttgcctctg gaagcctctc ttctttttct
     1261 ccagagtaag cggaggccag gggcccccgg cctctgctta atactaaaaa aaacagctgt
     1321 tgtcatagta atgattgggt ggaaacattc caggcctggg tggagaggct ttttgcttcc
     1381 tcttgcaaaa ccacactgac attccaggcc tgggtggaga ggctttttgc ttcctcttgc
     1441 aaaaccacac tgccctctgg agggcagttg cctagcaact aattaaaaga ggatgtcgca
     1501 cggccagctg cggtcagtta gtcacttcct gcttaactga cttgacattt tctattttaa
     1561 gagtcgggag gaaaattact gtgttggagg ccctccgcca tcttctgaag ctgaatcgaa
     1621 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1681 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1741 cttatcatgt ctggatctga tatcatcgtc gacattgatt attgactagt tattaatagt
     1801 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     1861 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1921 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1981 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     2041 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     2101 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     2161 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     2221 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gsgcgcgccr
     2281 ggsggggggg gggggggggg gggggggggs ggggsgrggs ggrgrggkgc ggcggcagcc
     2341 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
     2401 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
     2461 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
     2521 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     2581 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
     2641 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
     2701 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     2761 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
     2821 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
     2881 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
     2941 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
     3001 cggcaggkgg gggtgccggg cggggcgggg ccgccycggg ccggggrggg ctcgggggrg
     3061 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
     3121 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
     3181 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
     3241 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
     3301 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     3361 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     3421 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
     3481 cattttggca aagaattcct cgaggctagc cgtcgaagaa ttctcacgcg tgccaccatg
     3541 gccagcaact tcacccagtt cgtgctggtg gacaacggcg gcaccggcga cgtgaccgtg
     3601 gcccccagca acttcgccaa cggcatcgcc gagtggatca gcagcaacag ccgcagccag
     3661 gcctacaagg tgacctgcag cgtgcgccag agcagcgccc agaaccgcaa gtacaccatc
     3721 aaggtggagg tgcccaaggg cgcctggcgc agctacctga acatggagct gaccatcccc
     3781 atcttcgcca ccaacagcga ctgcgagctg atcgtgaagg ccatgcaggg cctgctgaag
     3841 gacggcaacc ccatccccag cgccatcgcc gccaacagcg gcatctacgg cggcgctgga
     3901 ggtgcaggtg gatccatggc cagcaacttc acccagttcg tgctggtgga caacggcggc
     3961 accggcgacg tgaccgtggc ccccagcaac ttcgccaacg gcatcgccga gtggatcagc
     4021 agcaacagcc gcagccaggc ctacaaggtg acctgcagcg tgcgccagag cagcgcccag
     4081 aaccgcaagt acaccatcaa ggtggaggtg cccaagggcg cctggcgcag ctacctgaac
     4141 atggagctga ccatccccat cttcgccacc aacagcgact gcgagctgat cgtgaaggcc
     4201 atgcagggcc tgctgaagga cggcaacccc atccccagcg ccatcgccgc caacagcggc
     4261 atctacggcg gcgctggagg tgcaggtgga tccgtgagca agggcgagga gctggtcggc
     4321 gcgccaacaa gtttgtacaa aaaagttggc atgtcgggag gtggtgtgat tcgtggcccc
     4381 gcagggaaca acgattgccg catctacgtg ggtaacttac ctccagacat ccgaaccaag
     4441 gacattgagg acgtgttcta caaatacggc gctatccgcg acatcgacct caagaatcgc
     4501 cgcgggggac cgcccttcgc cttcgttgag ttcgaggacc cgcgagacgc ggaagacgcg
     4561 gtgtatggtc gcgacggcta tgattacgat gggtaccgtc tgcgggtgga gtttcctcga
     4621 agcggccgtg gaacaggccg aggcggcggc gggggtggag gtggcggagc tccccgaggt
     4681 cgctatggcc ccccatccag gcggtctgaa aacagagtgg ttgtctctgg actgcctcca
     4741 agtggaagtt ggcaggattt aaaggatcac atgcgtgaag caggtgatgt atgttatgct
     4801 gatgtttacc gagatggcac tggtgtcgtg gagtttgtac ggaaagaaga tatgacctat
     4861 gcagttcgaa aactggataa cactaagttt agatctcatg agggagaaac tgcctacatc
     4921 cgggttaaag ttgatgggcc cagaagtcca agttatggaa gatctcgatc tcgaagccgt
     4981 agtcgtagca gaagccgtag cagaagcaac agcaggagtc gcagttactc cccaaggaga
     5041 agcagaggat caccacgcta ttctccccgt catagcagat ctcgctctcg tacataccca
     5101 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     5161 gattctacga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     5221 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     5281 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     5341 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     5401 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     5461 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     5521 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     5581 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     5641 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     5701 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     5761 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     5821 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag
     5881 taatgaacta gtaccggtta agtcgacaat caacgcgtta agtcgacagg ccgctcgata
     5941 agcttgatat cgaattccgc cccccccccc tctccctccc ccccccctaa cgttactggc
     6001 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     6061 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     6121 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     6181 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     6241 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     6301 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     6361 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     6421 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     6481 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     6541 tatggccaca accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     6601 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     6661 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     6721 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6781 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6841 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6901 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6961 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     7021 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     7081 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     7141 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     7201 ccatgcatcg atgatctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     7261 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     7321 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     7381 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     7441 ctggggatgc ggtgggctct atggcttctg aggcggaaag aacctgcagc ccaagcttgg
     7501 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7561 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7621 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     7681 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7801 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7861 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa
     7921 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7981 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc
     8041 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8101 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8161 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8221 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8281 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8341 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8401 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8461 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8521 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8581 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8641 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8701 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8761 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8821 ttacgcgcag aaaaaaagga tctcaag
//
