LOCUS       sequence-472025-        9948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lbr mCherry fusion protein with MCP domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Bhat P, Chow A, Emert B, Ettlin O, Quinodoz SA, Strehle M, Takei Y, 
            Burr A, Goronzy IN, Chen AW, Huang W, Ferrer JLM, Soehalim E, Goh 
            ST, Chari T, Sullivan DK, Blanco MR, Guttman M
  TITLE     Genome organization around nuclear speckles drives mRNA splicing 
            efficiency.
  JOURNAL   Nature. 2024 May;629(8014):1165-1173. doi: 
            10.1038/s41586-024-07429-6. Epub 2024 May 8.
  PUBMED    38720076
REFERENCE   2  (bases 1 to 9948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07429-6"; journalName: "Nature"; date: 
            "2024-05"; volume: "629"; issue: "8014"; pages: "1165-1173"
COMMENT     Sequence Label: pCAG-MCP-Lbr-V5-mCherry
FEATURES             Location/Qualifiers
     source          1..9948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(41..629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(121..140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(800..1591,1592..1660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1423..1442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     polyA_signal    2253..2387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        2401..2780
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3058..4074
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3998..4020
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4082..4101
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4162..4171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4957..4981
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6827..6851)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6859..6900
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6901..7611
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7046..7064)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7314..7333
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(7698..8272,8273..8275,8276..8284)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7865..7882)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8092..8111
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8285..8884
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8285..8304)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8781..8801
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8965..8982)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8971..9195
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9242..9258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9242..9258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9255..9277)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9266..9282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9290..9296,9297..9314,9315..9320))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9335..9356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9415..9610
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9461..9596
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9616..9750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9818..9835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
       61 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      121 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      181 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      241 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      301 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      361 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      421 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      481 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      781 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      841 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
      901 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
      961 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1021 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1081 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1141 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1201 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1261 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1321 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1381 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1441 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1501 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1561 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1621 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatgac
     1681 cgctttggaa aaacaaagac tgtatttcct ggaaattaat gtttattcaa taaactgtgt
     1741 attcagctat attcacatag tggtgaggct gaaatgaggc gggaagaggc ggttggggct
     1801 taattatatc aatttgggtg gccccacagc gcctccaagg cgccagtcct gttttgacaa
     1861 gttgcctctg gaagcctctc ttctttttct ccagagtaag cggaggccag gggcccccgg
     1921 cctctgctta atactaaaaa aaacagctgt tgtcatagta atgattgggt ggaaacattc
     1981 caggcctggg tggagaggct ttttgcttcc tcttgcaaaa ccacactgac attccaggcc
     2041 tgggtggaga ggctttttgc ttcctcttgc aaaaccacac tgccctctgg agggcagttg
     2101 cctagcaact aattaaaaga ggatgtcgca cggccagctg cggtcagtta gtcacttcct
     2161 gcttaactga cttgacattt tctattttaa gagtcgggag gaaaattact gtgttggagg
     2221 ccctccgcca tcttctgaag ctgaatcgaa ttaacttgtt tattgcagct tataatggtt
     2281 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2341 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga tatcatcgtc
     2401 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     2461 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     2521 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     2581 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     2641 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     2701 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2761 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     2821 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
     2881 tgggggcggg gggggggggg gggcgcgccr gggggggggg gggggggggg gggggggggg
     2941 ggggsgrggs ggrgrggkgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
     3001 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
     3061 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
     3121 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     3181 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
     3241 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
     3301 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
     3361 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     3421 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     3481 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     3541 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     3601 gcggggctcg ccgtgccggg cggggggtgg cggcaggkgg gggtgccggg cggggcgggg
     3661 ccgccycggg ccggggrggg ctcgggggrg gggcgcggcg gcccccggag cgccggcggc
     3721 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     3781 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     3841 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     3901 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     3961 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     4021 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     4081 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgaggctagc
     4141 cgtcgaagaa ttctcacgcg tgccaccatg gccagcaact tcacccagtt cgtgctggtg
     4201 gacaacggcg gcaccggcga cgtgaccgtg gcccccagca acttcgccaa cggcatcgcc
     4261 gagtggatca gcagcaacag ccgcagccag gcctacaagg tgacctgcag cgtgcgccag
     4321 agcagcgccc agaaccgcaa gtacaccatc aaggtggagg tgcccaaggg cgcctggcgc
     4381 agctacctga acatggagct gaccatcccc atcttcgcca ccaacagcga ctgcgagctg
     4441 atcgtgaagg ccatgcaggg cctgctgaag gacggcaacc ccatccccag cgccatcgcc
     4501 gccaacagcg gcatctacgg cggcgctgga ggtgcaggtg gatccatggc cagcaacttc
     4561 acccagttcg tgctggtgga caacggcggc accggcgacg tgaccgtggc ccccagcaac
     4621 ttcgccaacg gcatcgccga gtggatcagc agcaacagcc gcagccaggc ctacaaggtg
     4681 acctgcagcg tgcgccagag cagcgcccag aaccgcaagt acaccatcaa ggtggaggtg
     4741 cccaagggcg cctggcgcag ctacctgaac atggagctga ccatccccat cttcgccacc
     4801 aacagcgact gcgagctgat cgtgaaggcc atgcagggcc tgctgaagga cggcaacccc
     4861 atccccagcg ccatcgccgc caacagcggc atctacggcg gcgctggagg tgcaggtgga
     4921 tccgtgagca agggcgagga gctggtcggc gcgccaacaa gtttgtacaa aaaagttggc
     4981 atgccaagta ggaaatttgc cgatggtgaa gtggtaagag gtcgatggcc tgggagttca
     5041 ctttattatg aagtagaaat tctgagccac gacagcacct cccagcttta cactgtgaag
     5101 tataaagatg gaacagagct tgaattgaaa gagaatgata ttaagccttt aacttccttt
     5161 aggcaaagga aaggtggctc aacttccagt tccccttcca gacgccgagg gagtcgatca
     5221 aggtcacgct cccgatcccc tggtcgacca cctaaaagtg cccgccgatc tgcttctgct
     5281 tcccaccagg ccgacattaa ggaagcaagg agggaagtgg aagttaaatt gactccgctg
     5341 attctgaagc catttggaaa tagcatcagc agatataatg gggagcctga gcatattgag
     5401 agaaatgacg cacctcataa aaatacacag gaaaaattca gtttgtcaca agaaagcagt
     5461 tacatagcaa cacagtatag ccttcgtcca agaagagaag aagtcaaatt aaaagaaata
     5521 gattctaagg aagaaaaata cgttgcaaaa gaactggcag tgagaacctt tgaagtgacc
     5581 cccatccggg caaaggactt ggagtttgga ggagtacctg gtgtgtttct catcatgttt
     5641 ggcctgcctg tgttcctctt cctgttgctg ttgatgtgta aacagaaaga tcccagtctt
     5701 ctgaatttcc ctcctccttt gccagctttg tatgagttat gggaaaccag agtatttggg
     5761 gtctacctcc tgtggttttt gattcaagtc ctgttctacc tactgccaat tggaaaggtt
     5821 gtagaaggaa cgcctcttat tgatggaaga agactcaagt atagattaaa tggattctat
     5881 gcttttatcc tgacatctgc agtcatcgga acatctctct tccagggcgt agagtttcat
     5941 tacgtgtaca gtcattttct tcagtttgca cttgcggcca ctgttttttg tgtggtcttg
     6001 agtgtgtatc tctacatgcg ctctttgaaa gcgccccgga atgacctgtc gcctgccagc
     6061 tctggaaatg ctgtctatga tttcttcatt ggccgtgaat taaaccctcg aattggtact
     6121 tttgatctca aatacttttg tgaattgcgc cccggattga ttggatgggt ggttattaac
     6181 ttggtgatgc ttttggctga aatgaaaata caggaccgcg ctgttccatc cttggccatg
     6241 attttagtta atagtttcca gcttctctat gtggtggatg ctctctggaa tgaggaagcg
     6301 ttgttgacga ccatggacat catccacgat ggatttggat tcatgctggc ttttggagac
     6361 ttggtgtggg ttccctttat ttacagcttc caagcctttt atttagtcag tcatccaaat
     6421 gaagtgtctt ggccaatggc ttctctaatt attgttctga aactttgtgg ttatgtaatc
     6481 ttccgaggtg caaattctca gaaaaatgca ttccggaaaa atcccagtga tccaaagctt
     6541 gcacatttaa aaaccattca tacttcaacg ggaaaaaatc ttctagtttc tggatggtgg
     6601 ggctttgttc gccaccccaa ttacttgggt gatctcatca tggccttggc gtggtccctc
     6661 ccatgtggtt ttaaccacat tctgccttat ttctacataa tttatttcac catgttgctt
     6721 gtccaccgag aagctcgtga cgagtaccac tgtaagaaga aatacggcgt ggcttgggaa
     6781 aagtactgtc agcgtgtgcc ctaccgtata tttccataca tctactaccc aactttcttg
     6841 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     6901 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     6961 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     7021 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     7081 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     7141 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     7201 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     7261 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     7321 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     7381 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     7441 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     7501 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     7561 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta gtaatgaact
     7621 agtaccggtt aagtcgacaa tcaacgcgtt aagtcgacag gccgctcgat aagcttgata
     7681 tcgaattccg cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     7741 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     7801 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     7861 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     7921 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     7981 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     8041 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     8101 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     8161 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     8221 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     8281 aaccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     8341 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     8401 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     8461 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     8521 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     8581 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     8641 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     8701 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     8761 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     8821 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     8881 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
     8941 gatgatctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     9001 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     9061 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     9121 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     9181 cggtgggctc tatggcttct gaggcggaaa gaacctgcag cccaagcttg gcgtaatcat
     9241 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9301 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9361 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct
     9421 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9481 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9541 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9601 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9661 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9721 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
     9781 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9841 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9901 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagc
//
