LOCUS       sequence-472026-        8520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion 
            protein with MCP domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Bhat P, Chow A, Emert B, Ettlin O, Quinodoz SA, Strehle M, Takei Y, 
            Burr A, Goronzy IN, Chen AW, Huang W, Ferrer JLM, Soehalim E, Goh 
            ST, Chari T, Sullivan DK, Blanco MR, Guttman M
  TITLE     Genome organization around nuclear speckles drives mRNA splicing 
            efficiency.
  JOURNAL   Nature. 2024 May;629(8014):1165-1173. doi: 
            10.1038/s41586-024-07429-6. Epub 2024 May 8.
  PUBMED    38720076
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07429-6"; journalName: "Nature"; date: 
            "2024-05"; volume: "629"; issue: "8014"; pages: "1165-1173"
COMMENT     Sequence Label: pCAG-MCP-dNS-SRRM1-V5-mCherry
FEATURES             Location/Qualifiers
     source          1..8520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(41..629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(121..140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(800..1591,1592..1660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1423..1442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     polyA_signal    2253..2387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        2401..2780
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3058..4074
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3998..4020
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4082..4101
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4162..4171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4957..4981
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5399..5423)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5431..5472
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5473..6183
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5618..5636)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5886..5905
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(6270..6844,6845..6847,6848..6856)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6437..6454)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6664..6683
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6857..7456
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6857..6876)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7353..7373
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7537..7554)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7543..7767
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7814..7830)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7814..7830)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7827..7849)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7838..7854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7862..7868,7869..7886,7887..7892))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7907..7928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7987..8182
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8033..8168
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8188..8322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8390..8407)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
       61 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      121 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      181 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      241 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      301 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      361 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      421 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      481 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      781 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      841 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
      901 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
      961 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1021 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1081 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1141 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1201 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1261 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1321 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1381 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1441 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1501 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1561 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1621 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatgac
     1681 cgctttggaa aaacaaagac tgtatttcct ggaaattaat gtttattcaa taaactgtgt
     1741 attcagctat attcacatag tggtgaggct gaaatgaggc gggaagaggc ggttggggct
     1801 taattatatc aatttgggtg gccccacagc gcctccaagg cgccagtcct gttttgacaa
     1861 gttgcctctg gaagcctctc ttctttttct ccagagtaag cggaggccag gggcccccgg
     1921 cctctgctta atactaaaaa aaacagctgt tgtcatagta atgattgggt ggaaacattc
     1981 caggcctggg tggagaggct ttttgcttcc tcttgcaaaa ccacactgac attccaggcc
     2041 tgggtggaga ggctttttgc ttcctcttgc aaaaccacac tgccctctgg agggcagttg
     2101 cctagcaact aattaaaaga ggatgtcgca cggccagctg cggtcagtta gtcacttcct
     2161 gcttaactga cttgacattt tctattttaa gagtcgggag gaaaattact gtgttggagg
     2221 ccctccgcca tcttctgaag ctgaatcgaa ttaacttgtt tattgcagct tataatggtt
     2281 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2341 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga tatcatcgtc
     2401 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     2461 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     2521 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     2581 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     2641 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     2701 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2761 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     2821 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
     2881 tgggggcggg gggggggggg gggcgcgccr gggggggggg gggggggggg gggggggggg
     2941 ggggsgrggs ggrgrggkgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
     3001 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
     3061 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
     3121 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     3181 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
     3241 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
     3301 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
     3361 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     3421 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     3481 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     3541 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     3601 gcggggctcg ccgtgccggg cggggggtgg cggcaggkgg gggtgccggg cggggcgggg
     3661 ccgccycggg ccggggrggg ctcgggggrg gggcgcggcg gcccccggag cgccggcggc
     3721 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     3781 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     3841 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     3901 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     3961 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     4021 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     4081 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgaggctagc
     4141 cgtcgaagaa ttctcacgcg tgccaccatg gccagcaact tcacccagtt cgtgctggtg
     4201 gacaacggcg gcaccggcga cgtgaccgtg gcccccagca acttcgccaa cggcatcgcc
     4261 gagtggatca gcagcaacag ccgcagccag gcctacaagg tgacctgcag cgtgcgccag
     4321 agcagcgccc agaaccgcaa gtacaccatc aaggtggagg tgcccaaggg cgcctggcgc
     4381 agctacctga acatggagct gaccatcccc atcttcgcca ccaacagcga ctgcgagctg
     4441 atcgtgaagg ccatgcaggg cctgctgaag gacggcaacc ccatccccag cgccatcgcc
     4501 gccaacagcg gcatctacgg cggcgctgga ggtgcaggtg gatccatggc cagcaacttc
     4561 acccagttcg tgctggtgga caacggcggc accggcgacg tgaccgtggc ccccagcaac
     4621 ttcgccaacg gcatcgccga gtggatcagc agcaacagcc gcagccaggc ctacaaggtg
     4681 acctgcagcg tgcgccagag cagcgcccag aaccgcaagt acaccatcaa ggtggaggtg
     4741 cccaagggcg cctggcgcag ctacctgaac atggagctga ccatccccat cttcgccacc
     4801 aacagcgact gcgagctgat cgtgaaggcc atgcagggcc tgctgaagga cggcaacccc
     4861 atccccagcg ccatcgccgc caacagcggc atctacggcg gcgctggagg tgcaggtgga
     4921 tccgtgagca agggcgagga gctggtcggc gcgccaacaa gtttgtacaa aaaagcaggc
     4981 tccaccatgg acgcgggatt tttccgcgga acaagtgcag aacaggataa tcggttcagc
     5041 aacaaacaga agaaactact gaagcagctg aaatttgcag aatgcctaga aaaaaaggtg
     5101 gacatgagca aagtaaattt ggaggttata aagccttgga taacaaaaag agtaacggaa
     5161 atccttgggt ttgaagatga tgttgtgatt gagtttatat tcaaccagct ggaagtgaag
     5221 aatccagact ccaaaatgat gcaaatcaac ctgactggat ttttgaatgg aaaaaatgct
     5281 cgagaattta tgggagaact gtggcccctg ctgctaagtg cacaagaaaa catcgcggga
     5341 atcccttctg ctttcctaga actgaagaaa gaagaaataa aacaaagaca gattgaacaa
     5401 ccagctttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     5461 ctcgattcta cgatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     5521 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     5581 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     5641 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     5701 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     5761 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     5821 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     5881 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     5941 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     6001 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     6061 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     6121 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     6181 tagtaatgaa ctagtaccgg ttaagtcgac aatcaacgcg ttaagtcgac aggccgctcg
     6241 ataagcttga tatcgaattc cgcccccccc ccctctccct cccccccccc taacgttact
     6301 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     6361 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     6421 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     6481 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     6541 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     6601 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     6661 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     6721 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     6781 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     6841 taatatggcc acaaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     6901 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     6961 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     7021 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     7081 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     7141 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     7201 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     7261 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     7321 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     7381 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     7441 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     7501 accccatgca tcgatgatct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     7561 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     7621 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     7681 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     7741 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaacctgc agcccaagct
     7801 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7861 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7921 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7981 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     8041 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8101 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8161 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     8221 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8281 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     8341 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8401 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8461 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
//
