LOCUS       sequence-47204-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3070-01
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..761
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ggatccccgg gttaattaac ggtgacggtg ctggtttaat taacatgtct aaaggtgaag
       61 aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt aatggtcaca
      121 aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg accttaaaat
      181 ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact actttaggtt
      241 atggtttgat gtgttttgct agatacccag atcatatgaa acaacatgac tttttcaagt
      301 ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat gacggtaact
      361 acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga atcgaattaa
      421 aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa tacaactata
      481 actctcacaa tgtttacatc atggctgaca aacaaaagaa tggtatcaaa gttaacttca
      541 aaattagaca caacattgaa gatggttctg ttcaattagc tgaccattat caacaaaata
      601 ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc tatcaatctg
      661 ccttatccaa agatccaaac gaaaagagag accacatggt cttgttagaa tttgttactg
      721 ctgctggtat tacccatggt atggatgaat tgtacaaata aggcgcgcca cttctaaata
      781 agcgaatttc ttatgattta tgattttt
//
