LOCUS       sequence-472096-       12696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express tagBFP-2A-ICP0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, 
            Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig 
            T, Dolken L, Lomonte P, Fachinetti D, Manel N
  TITLE     Centromeric DNA amplification triggered by viral proteins activates 
            nuclear cGAS.
  JOURNAL   Cell. 2025 May 29:S0092-8674(25)00556-2. doi: 
            10.1016/j.cell.2025.05.008.
  PUBMED    40460826
REFERENCE   2  (bases 1 to 12696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 29:S0092-8674(25)00556-2. doi: 10.1016/j.cell.2025.05.008."
COMMENT     Sequence Label: pTRIP-SFFV-tagBFP-2A-ICP0
FEATURES             Location/Qualifiers
     source          1..12696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             7100..7280
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8808..8827)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9041..9060)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9160..9182
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9220..9238)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9306..9410
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9411..9479,9480..10271)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9629..9648)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10442..11030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10931..10950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11184..11201
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11271..11291)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11276..11605
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11456..11591
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc catggagccc
     4501 cgccccggag cgagtacccg ccggcctgag ggccgccccc agcgcgagcc cgccccggat
     4561 gtctgggtgt ttccctgcga ccgagacctg ccggacagca gcgactctga ggcggagacc
     4621 gaagtggggg ggcgggggga cgccgaccac catgacgacg actccgcctc cgaggcggac
     4681 agcacggaca cggaactgtt cgagacgggg ctgctggggc cgcagggcgt ggatgggggg
     4741 gcggtctcgg gggggagccc cccccgcgag gaagaccccg gcagttgcgg gggcgccccc
     4801 cctcgagagg acggggggag cgacgagggc gacgtgtgcg ccgtgtgcac ggatgagatc
     4861 gcgccccacc tgcgctgcga caccttcccg tgcatgcacc gcttctgcat cccgtgcatg
     4921 aaaacctgga tgcaattgcg caacacctgc ccgctgtgca acgccaagct ggtgtacctg
     4981 atagtgggcg tgacgcccag cgggtcgttc agcaccatcc cgatcgtgaa cgacccccag
     5041 acccgcatgg aggccgagga ggccgtcagg gcgggcacgg ccgtggactt tatctggacg
     5101 ggcaatcagc ggttcgcccc gcggtacctg accctggggg ggcacacggt gagggccctg
     5161 tcgcccaccc acccggagcc caccacggac gaggatgacg acgacctgga cgacgcagac
     5221 tacgtaccgc ccgccccccg ccggacgccc cgcgcccccc cacgcagagg cgccgccgcg
     5281 ccccccgtga cgggcggggc gtctcacgca gccccccagc cggccgcggc tcggacagcg
     5341 cccccctcgg cgcccatcgg gccacacggc agcagtaaca ccaacaccac caccaacagc
     5401 agcggcggcg gcggctcccg ccagtcgcga gccgcggcgc cgcggggggc gtctggcccc
     5461 tccggggggg ttggggttgg ggttggggtt gttgaagcgg aggcggggcg gccgaggggc
     5521 cggacgggcc cccttgtcaa cagacccgcc ccccttgcaa acaacagaga ccccatagtg
     5581 atcagcgact cccccccggc ctctccccac aggccccccg cggcgcccat gccaggctcc
     5641 gccccccgcc ccgggccccc cgcgtccgcg gccgcgtcgg gacccgcgcg cccccgcgcg
     5701 gccgtggccc cgtgcgtgcg agcgccgcct ccggggcccg gcccccgcgc cccggccccc
     5761 ggggcggagc cggccgcccg ccccgcggac gcgcgccgtg tgccccagtc gcactcgtcc
     5821 ctggctcagg ccgcgaacca agaacagagt ctgtgccggg cgcgtgcgac ggtggcgcgc
     5881 ggctcggggg ggccgggcgt ggagggtggg cacgggccct cccgcggcgc cgccccctcc
     5941 ggcgccgccc cgctcccctc cgccgcctct gtcgagcagg aggcggcggt gcgtccgagg
     6001 aagaggcgcg ggtcgggcca ggaaaacccc tccccccagt ccacgcgtcc ccccctcgcg
     6061 ccggcagggg ccaagagggc ggcgacgcac cccccctccg actcagggcc gggggggcgc
     6121 ggccagggtg ggcccgggac ccccctgacg tcctcggcgg cctccgcctc ttcctcctct
     6181 gcctcttcct cctcggcccc gacccccgcg ggggccgcct cttccgccgc cggggccgcg
     6241 tcctcctccg cttccgcctc ctcgggcggg gccgtcggtg ccctgggagg gagacaagag
     6301 gaaacctccc tcggcccccg cgctgcttct gggccgcggg ggccgaggaa gtgtgcccgg
     6361 aagacgcgcc acgcggagac ttccggggcc gtccccgcgg gcggcctcac gcgctacctg
     6421 cccatctcgg gggtctctag cgtggtcgcc ctgtcgcctt acgtgaacaa gactatcacg
     6481 ggggactgcc tgcccatcct ggacatggag acggggaaca tcggggcgta cgtggtcctg
     6541 gtggaccaga cgggaaacat ggcgacccgg ctgcgggccg cggtccccgg ctggagccgc
     6601 cgcaccctgc tccccgagac cgcgggtaac cacgtgatgc cccccgagta cccgacggcc
     6661 cccgcgtcgg agtggaacag cctctggatg acccccgtgg ggaacatgct gttcgaccag
     6721 ggcaccctag tgggcgccct ggacttccgc agcctgcggt ctcggcaccc gtggtccggg
     6781 gagcaggggg cgtcgacccg ggacgaggga aaacaataaa ctagtgtcga cctcgagacc
     6841 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     6901 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     6961 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7021 tggaagggct aattcactcc caacgaagac aaaatcgtcg agagatgctg catataagca
     7081 gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7141 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7201 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7261 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7321 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tataatagat ttagcaggaa
     7381 ttgaactagg agtggagcac acaggcaaag ctgcagaagt acttggaaga agccaccaga
     7441 gatactcacg attctgcaca tacctggcta atcccagatc ctaaggatta cattaagttt
     7501 actaacattt atataatgat ttatagttta aagtataaac ttatctaatt tactattctg
     7561 acagatatta attaatcctc aaatatcata agagatgatt actattatcc ccatttaaca
     7621 caagaggaaa ctgagaggga aagatgttga agtaattttc ccacaattac agcatccgtt
     7681 agttacgact ctatgatctt ctgacacaaa ttccatttac tcctcaccct atgactcagt
     7741 cgaatatatc aaagttatgg acattatgct aagtaacaaa ttaccctttt atatagtaaa
     7801 tactgagtag attgagagaa gaaattgttt gcaaacctga atagcttcaa gaagaagaga
     7861 agtgaggata agaataacag ttgtcattta acaagtttta acaagtaact tggttagaaa
     7921 gggattcaaa tgcataaagc aagggataaa tttttctggc aacaagacta tacaatataa
     7981 ccttaaatat gacttcaaat aattgttgga acttgataaa actaattaaa tattattgaa
     8041 gattatcaat attataaatg taatttactt ttaaaaaggg aacatagaaa tgtgtatcat
     8101 tagagtagaa aacaatcctt attatcacaa tttgtcaaaa caagtttgtt attaacacaa
     8161 gtagaatact gcattcaatt aagttgactg cagattttgt gttttgttaa aattagaaag
     8221 agataacaac aatttgaatt attgaaagta acatgtaaat agttctacat acgttctttt
     8281 gacatcttgt tcaatcattg atcgaagttc tttatcttgg aagaatttgt tccaaagact
     8341 ctgaaataag gaaaacaatc tattatatag tctcacacct ttgttttact tttagtgatt
     8401 tcaatttaat aatgtaaatg gttaaaattt attcttctct gagatcattt cacattgcag
     8461 atagaaaacc tgagactggg gtaattttta ttaaaatcta atttaatctc agaaacacat
     8521 ctttattcta acatcaattt ttccagtttg atattatcat ataaagtcag ccttcctcat
     8581 ctgcaggttc cacaacaaaa atccaaccaa ctgtggatca aaaatattgg gaaaaaatta
     8641 aaaatagcaa tacaacaata aaaaaataca aatcagaaaa acagcacagt ataacaactt
     8701 tatttagcat ttacaatcta ttaggtatta taagtaatct agccagatcc tctacgccgg
     8761 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     8821 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     8881 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     8941 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     9001 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
     9061 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     9121 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     9181 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     9241 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     9301 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9361 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     9421 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     9481 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     9541 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     9601 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     9661 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     9721 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     9781 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     9841 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9901 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9961 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    10021 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    10081 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    10141 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    10201 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    10261 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    10321 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    10381 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    10441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10501 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    10561 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    10621 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    10681 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    10741 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    10801 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    10861 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10921 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10981 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    11041 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    11101 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    11161 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    11221 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
    11281 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    11341 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    11401 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
    11461 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    11521 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    11581 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
    11641 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
    11701 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
    11761 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
    11821 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
    11881 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
    11941 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
    12001 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
    12061 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
    12121 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
    12181 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
    12241 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
    12301 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
    12361 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
    12421 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
    12481 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
    12541 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
    12601 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
    12661 cggatctttg tgaaggaacc ttacttctgt ggtgtg
//
