LOCUS       sequence-472097-       11838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express tagBFP-2A-IE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, 
            Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig 
            T, Dolken L, Lomonte P, Fachinetti D, Manel N
  TITLE     Centromeric DNA amplification triggered by viral proteins activates 
            nuclear cGAS.
  JOURNAL   Cell. 2025 May 29:S0092-8674(25)00556-2. doi: 
            10.1016/j.cell.2025.05.008.
  PUBMED    40460826
REFERENCE   2  (bases 1 to 11838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 29:S0092-8674(25)00556-2. doi: 10.1016/j.cell.2025.05.008."
COMMENT     Sequence Label: pTRIP-SFFV-tagBFP-2A-IE1
FEATURES             Location/Qualifiers
     source          1..11838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      5189..5198
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             6242..6422
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7950..7969)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8183..8202)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8302..8324
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8362..8380)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8448..8552
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8553..8621,8622..9413)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8771..8790)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9584..10172
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10073..10092
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10326..10343
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10413..10433)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10418..10747
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10598..10733
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc cactagaatg
     4501 gagtcctctg ccaagagaaa gatggaccct gataatcctg acgagggccc ttcctccaag
     4561 gtgccacggc ccgagacacc cgtgaccaag gccacgacgt tcctgcagac tatgttgagg
     4621 aaggaggtta acagtcagct gagtctggga gacccgctgt ttccagagtt ggccgaagaa
     4681 tccctcaaaa cttttgaacg agtgaccgag gattgcaacg agaaccccga gaaagatgtc
     4741 ctggcagaac tcgtcaaaca gattaaggtt cgagtggaca tggtgcggca tagaatcaag
     4801 gagcacatgc tgaaaaaata tacccagacg gaagagaaat tcactggcgc ctttaatatg
     4861 atgggaggat gtttgcagaa tgccttagat atcttagata aggttcatga gcctttcgag
     4921 gagatgaagt gtattgggct aactatgcag agcatgtatg agaactacat tgtacctgag
     4981 gataagcggg agatgtggat ggcttgtatt aaggagctgc atgatgtgag caagggcgcc
     5041 gctaacaagt tggggggtgc actgcaggct aaggcccgtg ctaaaaagga tgaacttagg
     5101 agaaagatga tgtatatgtg ctacaggaat atagagttct ttaccaagaa ctcagccttc
     5161 cctaagacca ccaatggctg cagtcaggcc atggcggcat tgcagaactt gcctcagtgc
     5221 tcccctgatg agattatggc ttatgcccag aaaatattta agattttgga tgaggagaga
     5281 gacaaggtgc tcacgcacat tgatcacata tttatggata tcctcactac atgtgtggaa
     5341 acaatgtgta atgagtacaa ggtcactagt gacgcttgta tgatgaccat gtacgggggc
     5401 atctctctct taagtgagtt ctgtcgggtg ctgtgctgct atgtcttaga ggagactagt
     5461 gtgatgctgg ccaagcggcc tctgataacc aagcctgagg ttatcagtgt aatgaagcgc
     5521 cgcattgagg agatctgcat gaaggtcttt gcccagtaca ttctgggggc cgatcctctg
     5581 agagtctgct ctcctagtgt ggatgaccta cgggccatcg ccgaggagtc agatgaggaa
     5641 gaggctattg tagcctacac tttggccacc gctggtgcca gctcctctga ttctctggtg
     5701 tcacccccag agtcccctgt acccgcgact atccctctgt cctcagtaat tgtggctgag
     5761 aacagtgatc aggaagaaag tgagcagagt gatgaggaag aggaggaggg tgctcaggag
     5821 gagcgggagg acactgtgtc tgtcaagtct gagccagtgt ctgagataga ggaagttgcc
     5881 ccagaggaag aggaggatgg tgctgaggaa cccaccgcct ctggaggcaa gagcacccac
     5941 cctatggtga ctagaagcaa ggctgaccag taactcgaga cctagaaaaa catggagcaa
     6001 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     6061 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     6121 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6181 cccaacgaag acaaaatcgt cgagagatgc tgcatataag cagctgcttt ttgcttgtac
     6241 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6301 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6361 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6421 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6481 agagagtgag aggccttgac attataatag atttagcagg aattgaacta ggagtggagc
     6541 acacaggcaa agctgcagaa gtacttggaa gaagccacca gagatactca cgattctgca
     6601 catacctggc taatcccaga tcctaaggat tacattaagt ttactaacat ttatataatg
     6661 atttatagtt taaagtataa acttatctaa tttactattc tgacagatat taattaatcc
     6721 tcaaatatca taagagatga ttactattat ccccatttaa cacaagagga aactgagagg
     6781 gaaagatgtt gaagtaattt tcccacaatt acagcatccg ttagttacga ctctatgatc
     6841 ttctgacaca aattccattt actcctcacc ctatgactca gtcgaatata tcaaagttat
     6901 ggacattatg ctaagtaaca aattaccctt ttatatagta aatactgagt agattgagag
     6961 aagaaattgt ttgcaaacct gaatagcttc aagaagaaga gaagtgagga taagaataac
     7021 agttgtcatt taacaagttt taacaagtaa cttggttaga aagggattca aatgcataaa
     7081 gcaagggata aatttttctg gcaacaagac tatacaatat aaccttaaat atgacttcaa
     7141 ataattgttg gaacttgata aaactaatta aatattattg aagattatca atattataaa
     7201 tgtaatttac ttttaaaaag ggaacataga aatgtgtatc attagagtag aaaacaatcc
     7261 ttattatcac aatttgtcaa aacaagtttg ttattaacac aagtagaata ctgcattcaa
     7321 ttaagttgac tgcagatttt gtgttttgtt aaaattagaa agagataaca acaatttgaa
     7381 ttattgaaag taacatgtaa atagttctac atacgttctt ttgacatctt gttcaatcat
     7441 tgatcgaagt tctttatctt ggaagaattt gttccaaaga ctctgaaata aggaaaacaa
     7501 tctattatat agtctcacac ctttgtttta cttttagtga tttcaattta ataatgtaaa
     7561 tggttaaaat ttattcttct ctgagatcat ttcacattgc agatagaaaa cctgagactg
     7621 gggtaatttt tattaaaatc taatttaatc tcagaaacac atctttattc taacatcaat
     7681 ttttccagtt tgatattatc atataaagtc agccttcctc atctgcaggt tccacaacaa
     7741 aaatccaacc aactgtggat caaaaatatt gggaaaaaat taaaaatagc aatacaacaa
     7801 taaaaaaata caaatcagaa aaacagcaca gtataacaac tttatttagc atttacaatc
     7861 tattaggtat tataagtaat ctagccagat cctctacgcc ggacgcatcg tggccggcat
     7921 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     7981 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     8041 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     8101 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     8161 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     8221 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8281 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8341 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     8401 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8461 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8521 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     8581 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8641 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8701 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8761 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8821 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8881 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8941 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9001 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9061 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9121 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9181 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9241 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9301 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9361 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9421 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9481 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9541 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9601 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9661 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9721 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9781 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9841 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9901 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9961 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10021 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10081 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10141 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10201 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10261 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    10321 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    10381 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
    10441 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    10501 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    10561 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    10621 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    10681 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    10741 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
    10801 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
    10861 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
    10921 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
    10981 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
    11041 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
    11101 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
    11161 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
    11221 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
    11281 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
    11341 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
    11401 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
    11461 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
    11521 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
    11581 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
    11641 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
    11701 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
    11761 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
    11821 ccttacttct gtggtgtg
//
